TITLE

Analysis of non-crossover bivalents in pachytene cells from 10 normal men

AUTHOR(S)
Fei Sun; M. Oliver-Bonet; T. Liehr; H. Starke; P. Turek; E. Ko; A. Rademaker; R.H. Martin
PUB. DATE
September 2006
SOURCE
Human Reproduction;Sep2006, Vol. 21 Issue 9, p2335
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
BACKGROUND: Bivalents with no recombination foci (possible achiasmates) are unable to orient properly on the metaphase plate or to segregate chromosomes to daughter cells. Non-crossover bivalents are known to cause meiotic arrest in various organisms. METHODS: Individual non-crossover bivalents were identified in 886 pachytene cells (19 492 bivalents) from testicular biopsies of 10 normal men. Fluorescence staining combined with centromere-specific multicolour fluorescence in situ hybridization (cenM-FISH) was used to identify mismatch repair gene mutation of human mutL homologue 1 (MLH1) recombination foci along each bivalent synaptonemal complex (SC). RESULTS: A total of 60 autosomal non-crossovers (SCs without an MLH1 focus) were found, and of these, chromosomes 21 (2.1%) and 22 (1.7%) had a significantly higher proportion than chromosomes 11, 12, 19 (each 0.1%), 13 (0.2%), 14 (0.6%), 16 (0.5%) and 15, 17, 18, 20 (each 0.3%) (P < 0.05). Sex chromosome univalents had a frequency of 27%, higher than that observed in any autosomal bivalent (P < 0.0001). CONCLUSIONS: These results suggest that G-group chromosomes and sex chromosomes are most susceptible to having no recombination foci and thus would be more susceptible to non-disjunction during spermatogenesis. This is consistent with previous observations from sperm karyotyping and FISH analysis, which demonstrate that chromosomes 21 and 22 and the sex chromosomes have a significantly increased frequency of aneuploidy compared with other autosomes.
ACCESSION #
22528429

 

Related Articles

  • Segregation of sex chromosomes in spermatozoa of 46,XY/47,XXY men by multicolour fluorescence in-situ hybridization. Morel, Frédéric; Roux, Christophe; Bresson, Jean-Luc // MHR: Basic Science of Reproductive Medicine;Jun2000, Vol. 6 Issue 6 

    Studies the sex chromosome disomy and diploidy rates on ejaculated spermatozoa from patients with mosaic Klinefelter's syndrome using multicolor fluorescence in-situ hybridization. Frequency of sperm sex chromosome; Presentation of disomy and diploidy levels; Similarity of the gonosomal...

  • Impact of gonosomal mosaicism on embryo development. Magli, M. C.; Gianaroli, L.; Ferraretti, A. P.; Lagalla, C.; Crippa, A. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;2002 Supplement 2, Vol. 4 Issue S2, p18 

    Objective: The detection in peripheral blood of cell lines at low frequency with mosaicism of gonosomes could expose the affected individual to a high reproductive risk if the gonads are involved in the production of aneuploid cell lines. Therefore, preimplantation genetic diagnosis (PGD) for...

  • Robertsonian and reciprocal translocations. Gianaroli, L.; Magli, M. C.; Ferraretti, A. P.; Fortini, D.; Tabanelli, C. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;2002 Supplement 2, Vol. 4 Issue S2, p26 

    Objective: A high incidence of miscarriage and abnormal offspring is strongly associated with the presence of a balanced translocation caused by a predisposition for generating unbalanced gametes. The current risk of an abnormal conceptus is variable and depends on the chromosomes involved in...

  • A rapid technique for detecting chromosomal aneuploidy in early childhood by fluorescence in situ hybridisation. Tardy, Erika P.; Tóth, András; Tardy, E P; Tóth, A // European Journal of Pediatrics;2000, Vol. 159 Issue 12, p935 

    Assesses the feasibility of interphase fluorescence in situ hybridization on peripheral blood and buccal epithelial smears retro- and prospectively. Effect of autosomal aneuploidy on newborn infants; Assessment on the reliability of buccal smears; Impact of sex chromosome abnormalities.

  • The Human Genome Project - an opportunity for improved fetal and infant health. Williamson, R. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;2002 Supplement 2, Vol. 4 Issue S2, p10 

    The Human Genome Project is now complete. Gene analysis for mutations that cause serious single-gene disorders and for aneuploidy can be carried out on single cells. Most couples will avoid the birth of a child with severe handicap if possible. However. the organizational strategies required to...

  • Evolutionary implications of permanent odd polyploidy in the stable sexual, pentaploid of Rosa canina L. Lim, K. Y.; Werlemark, G.; Matyasek, R.; Bringloe, J. B.; Sieber, V.; El Mokadem, H.; Meynet, J.; Hemming, J.; Leitch, A. R.; Roberts, A. V. // Heredity;May2005, Vol. 94 Issue 5, p501 

    In Rosa canina (2n=5x=35), the pollen and ovular parents contribute, respectively, seven and 28 chromosomes to the zygote. At meiosis I, 14 chromosomes form seven bivalents and 21 chromosomes remain as univalents. Fluorescent in situ hybridization to mitotic and pollen mother cells (PMC) of R....

  • Risk assessment and segregation analysis in a pericentric inversion inv(6)(p23q25) carrier using FISH on decondensed sperm nuclei. Anton; Blanco; Egozcue; Vidal // Cytogenetic & Genome Research;2002, Vol. 97 Issue 3/4, p149 

    Fluorescent in situ hybridization (FISH) in decondensed sperm nuclei has been used to determine the percentage of normal/balanced or unbalanced spermatozoa produced by an inv(6)(p23q25) carrier, and the possible interchromosomal effect (ICE) of the reorganized chromosomes on other chromosome...

  • The Rapidly Evolving Centromere-Specific Histone Has Stringent Functional Requirements in Arabidopsis thaliana.  // Genetics;Oct2010, Vol. 186 Issue 2, p461 

    No abstract available.

  • Linear increase of diploidy in human sperm with age: A four-colour FISH study. Bosch, Mercè; Rajmil, Osvaldo; Martínez-Pasarell, Olga; Egozcue, Josep; Templado, Cristina // European Journal of Human Genetics;Jul2001, Vol. 9 Issue 7, p533 

    The aim of this study was to determine if donor age is associated with an increased incidence of diploidy and of disomy for the sex chromosomes and for chromosomes 6 and 21. We used simultaneous fluorescence in situ hybridisation (FISH) for chromosomes 6, 21, X and Y in sperm from 18 healthy...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics