TITLE

Procedure for increasing the detection responses of estrogens in LC–MS based on introduction of a nitrobenzene moiety followed by electron capture atmospheric pressure chemical ionization

AUTHOR(S)
Higashi, Tatsuya; Takayama, Naoki; Nishio, Tadashi; Taniguchi, Emi; Shimada, Kazutake
PUB. DATE
November 2006
SOURCE
Analytical & Bioanalytical Chemistry;Nov2006, Vol. 386 Issue 3, p658
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A practical procedure for determining estrogens in biological fluids has been studied using liquid chromatography–electron capture atmospheric pressure chemical ionization–mass spectrometry combined with derivatization. Among the commercially available reagents (4-nitrobenzoyl chloride, 2,4-dinitrofluorobenzene, 4-nitrobenzenesulfonyl chloride and 4-nitrobenzyl bromide), 4-nitrobenzenesulfonyl chloride was of the most practical use; it rapidly and quantitatively reacted with estrogens and increased the detection responses by 8–23 times. The derivatization method allowed the reproducible and accurate quantification of serum and urine estrone and estradiol of a pregnant woman, which is useful for diagnosis of the fetoplacental function, with small amounts (10 μl) of sample and a simple pretreatment procedure. [Figure not available: see fulltext.]
ACCESSION #
22458637

 

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