Longitudinal diffusion behavior of hemicyanine dyes across phospholipid vesicle membranes as studied by second-harmonic generation and fluorescence spectroscopies

Yamaguchi, Akira; Nakano, Masaki; Nochi, Kimihisa; Yamashita, Tomohisa; Morita, Kotaro; Teramae, Norio
November 2006
Analytical & Bioanalytical Chemistry;Nov2006, Vol. 386 Issue 3, p627
Academic Journal
The adsorption and longitudinal diffusion behaviors of a series of hemicyanine dyes to phospholipid vesicle membranes were studied by second-harmonic generation (SHG) and fluorescence spectroscopies. It was observed that the longitudinal diffusion of cationic hemicyanine dyes takes place immediately after the initial adsorption of these dyes to the outer surface of the vesicle membrane. In contrast, hardly any amount of a zwitterionic hemicyanine dye with a sulfonate group diffused across the vesicle membrane within the measurement time (<2000 s). Based on the difference in the time-course responses of SHG and fluorescence spectroscopies for all of the hemicyanine dyes tested, we propose that hydration of the sulfonate group is mainly responsible for the low diffusivity of the zwitterionic hemicyanine dye.


Related Articles

  • On the modulation of optical transmission spectra of thin dye layers by a supporting medium. Bortchagovsky, E. G.; Fischer, U. C. // Journal of Chemical Physics;9/15/2002, Vol. 117 Issue 11, p5384 

    This work is devoted to the analysis of the shape of transmission spectra of composite systems including a thin adsorbed layer of absorbing dye molecules. It is demonstrated, that different from the often observed absorption characteristics in the transmission spectra as determined by the...

  • Interfacial behavior of sulforhodamine 101 at the polarized water/1,2-dichloroethane interface studied by spectroelectrochemical techniques. Nagatani, Hirohisa; Suzuki, Shingo; Fermín, David J.; Girault, Hubert H.; Nakatani, Kiyoharu // Analytical & Bioanalytical Chemistry;Nov2006, Vol. 386 Issue 3, p633 

    The transfer mechanism of an amphoteric rhodamine, sulforhodamine 101 (SR101), across the polarized water/1,2-dichloroethane (DCE) interface was investigated using cyclic voltammetry, differential voltfluorometry and potential-modulated fluorescence (PMF) spectroscopy. The voltammetric response...

  • 2-{(E)-2-[(3E)-2-Chloro-3-{(2E)-2-[1,1-dimethyl-3-(3-phenylpropyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene]-ethylidene}cyclohex-1-en-1-yl]ethenyl}-1,1-dimethyl-3-(3-phenylpropyl)-1H-benzo[e]indolium Iodide. Owens, Eric A.; Tawney, Joseph G.; Henary, Maged M. // Molbank;Mar2014, Vol. 14 Issue 1, p1 

    In four synthetic steps we successfully prepared a red-shifted heptamethine cyanine dye (max = 825 nm in methanol) that could be very useful for biochemists and bioanalytical chemists for probing lipophilic environments, including the hydrophobic pockets of enzymes. The heptamethine dye...

  • Shock-induced fluorescence shift of rhodamine-6G dye in ethanol solution. Shen, X. A.; Gupta, Y. M. // Journal of Applied Physics;12/15/1991, Vol. 70 Issue 12, p7549 

    Presents information on a study which described an experimental method for measuring the time-resolved fluorescence spectrum of a dye in a liquid subjected to shock wave, uniaxial strain compression. Description of the method; Results and discussion; Conclusions.

  • Free-Standing Microcrystals of a Fluorescent Organic Dye: Preparation and Spectroscopic Study. Abyan, Mouhammad; BîrlǬ, Liliana; Bertorelle, Franck; Rodrigues, Fernanda; Fery-forgues, Suzanne // International Journal of Photoenergy;Jan2006, p1 

    Suspensions of microcrystals were obtained from an organic fluorescent dye, 4- n-octylamino-7-nitrobenz-2-oxa-1,3-diazole, by reprecipitation in water in the presence of various macromolecules: anionic or cationic PAMAM dendrimer, poly(acrylic acid, sodium salt), poly(acrylamide), and calf...

  • Characterisation of solid-state dyes and their use as tunable laser amplifiers. Lam, S.Y.; Damzen, M.J. // Applied Physics B: Lasers & Optics;Nov2003, Vol. 77 Issue 6/7, p577 

    This paper reports on the spectroscopic and photo physical characteristics of solid-state dyes and their use as tunable high-gain laser amplifiers. A comparison of the absorption and fluorescence spectra is made for the dyes pyrromethene 567, pyrromethene 597, pyrromethene 650, rhodamine 6G in...

  • CARS ultrafast light source is hands-free. Huber, Heinz P.; Zoppel, Sandra; Rimke, Ingo // Laser Focus World;Aug2009, Vol. 45 Issue 8, p47 

    The article focuses on the use of coherent anti-Stokes Raman scattering (CARS) as a nonlinear optical effects in overcoming the problems with using dyes in a two-photon-fluorescence microscopy in 2009. It states that the CARS process utilize a Stokes field at ωs and a pump field at the...

  • Characterisation of fluorescence background in dye tracing. Smart, C. C.; Karunaratne, K. C. // Environmental Geology;Aug2002, Vol. 42 Issue 5, p492 

    Tracing with fluorescent dyes is a core technique in karst hydrogeology. The primary criterion for evaluation of a dye trace is whether a tracer can be demonstrated to significantly exceed background concentrations. The critical level of significance is generally based on the judgement of the...

  • Carbon-fibre adsorbents. Theoretical principles of production and conduct of sorption processes. Fridman, L. // Fibre Chemistry;Mar2011, Vol. 42 Issue 5, p300 

    The principles for selecting the initial polymer fibres for production of CFA are formulated, the conditions of activation of CF are determined, a mechanism of pore structure formation is proposed, and ways of regulating and varying the chemical state of the surface are demonstrated. The...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics