TITLE

Potential-step coulometry of d-glucose using a novel FAD-dependent glucose dehydrogenase

AUTHOR(S)
Tsujimura, Seiya; Kojima, Shinki; Ikeda, Tokuji; Kano, Kenji
PUB. DATE
November 2006
SOURCE
Analytical & Bioanalytical Chemistry;Nov2006, Vol. 386 Issue 3, p645
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This paper describes the construction and characterization of a batch-type coulometric system for the detection of d-glucose using a novel FAD-dependent glucose dehydrogenase. In order to overcome the problem of interferents, such as ascorbate and urate, a potential-step method was proposed to separate the electrolysis reactions of interferents and d-glucose by selecting a mediator possessing an appropriate formal potential. The rapid oxidative consumption of the interferents proceeded in the first step, whereas the mediator and glucose remained reduced. In the second step, the mediator was immediately oxidized, and subsequent bioelectrocatalytic oxidation of d-glucose occurred with the aid of aldose 1-epimerase. In this study, potassium octacyanomolybdate (IV) with a formal potential of 0.6 V vs. Ag|AgCl was chosen as a mediator, and the first and second electrolysis potentials were set at 0.4 V and 0.8 V, respectively, by considering the heterogeneous electron-transfer kinetics and the potential window. The background-corrected response in charge corresponded to 99±2 % efficiency in terms of the amount of d-glucose.
ACCESSION #
22458599

 

Related Articles

  • Comparative Kinetic Study of D-Glucose Oxidation by Ruthenium(III) Compounds Catalyzed by FAD-Dependent Glucose Oxidase and PQQ-Dependent Glucose Dehydrogenase. Ivanova, E. V.; Ershov, A. Yu.; Laurinavi&vcaron;ius, V.; Meskus, R.; Ryabov, A. D. // Biochemistry (00062979);Apr2003, Vol. 68 Issue 4, p407 

    The comparative kinetic study of two glucose oxidizing enzymes, FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase, is presented in the artificial electron transfer mediator system based on ruthenium(III) compounds. It is demonstrated that FAD-dependent glucose oxidase and...

  • Mediated electron transfer of cellobiose dehydrogenase and glucose oxidase at osmium polymer-modified nanoporous gold electrodes. Salaj-Kosla, Urszula; Scanlon, Micheál; Baumeister, Tobias; Zahma, Kawah; Ludwig, Roland; Conghaile, Peter; MacAodha, Domhnall; Leech, Dónal; Magner, Edmond // Analytical & Bioanalytical Chemistry;Apr2013, Vol. 405 Issue 11, p3823 

    Nanoporous and planar gold electrodes were utilised as supports for the redox enzymes Aspergillus niger glucose oxidase (GOx) and Corynascus thermophilus cellobiose dehydrogenase ( CtCDH). Electrodes modified with hydrogels containing enzyme, Os-redox polymers and the cross-linking agent...

  • Investigation of bioelectrocatalytic systems with PQQ-dependent GDH and carbonaceous materials. Voitechovič, Edita; Razumienė, Julija; šakinytė, Ieva; Barkauskas, Jurgis // Biologija;2010, Issue 1-4, p83 

    No abstract available.

  • Electrolysis for the benign conversion of renewable feedstocks. Hans J. Schäfer; Michael Harenbrock; Elisabeth Klocke; Mark Plate; Andreas Weiper-Idelmann // Pure & Applied Chemistry;Nov2007, Vol. 79 Issue 11, p2047 

    Pure Appl. Chem. 79, 1831-2100. An issue of reviews and research papers based on lectures presented at the 1st International IUPAC Conference on Green-Sustainable Chemistry, held in Dresden, Germany, 10-15 September 2006.A large variety of C,C-bond forming reactions and functional group...

  • Stripping coulometric determination of cadmium, lead and copper at modified screen-printed electrodes. Navolotskaya, D. V.; Ermakov, S. S.; Egorova, E. A.; Nikolaev, K. G. // Vestnik Sankt-Peterburgskogo universiteta, Seriia 7: Geologia, G;2013, Issue 2, p136 

    A rapid and cheap technique of simultaneous stripping coulometric (SC) determination of cadmium, lead and copper at preliminary mercury-modified screen-printed electrodes is proposed. Optimal conditions of stripping coulometric measurements are selected, i. e. composition of supporting...

  • Stripping coulometric determination of cadmium, lead and copper at modified screen-printed electrodes. Navolotskaya, D. V.; Ermakov, S. S.; Egorova, E. A.; Nikolaev, K. G. // Vestnik Sankt-Peterburgskogo universiteta, Seriia 7: Geologia, G;2013, Issue 2, p162 

    A rapid and cheap technique of simultaneous stripping coulometric (SC) determination of cadmium, lead and copper at preliminary mercury-modified screen-printed electrodes is proposed. Optimal conditions of stripping coulometric measurements are selected, i. e. composition of supporting...

  • Electron transfer in crystals of the binary and ternary complexes of methylamine dehydrogenase with amicyanin and cytochrome c551i as detected by EPR spectroscopy. Davide Ferrari; Marilena Di Valentin; Donatella Carbonera; Angelo Merli; Zhi-wei Chen; F. Scott Mathews; Victor L. Davidson; Gian Luigi Rossi // Journal of Biological Inorganic Chemistry;Mar2004, Vol. 9 Issue 2, p231 

    EPR studies of the methylamine dehydrogenase (MADH)?amicyanin and MADH?amicyanin?cytochrome c551i crystalline complexes have been performed on randomly oriented microcrystals before and after exposure to the substrate, methylamine, as a function of pH. The results show that EPR signals from the...

  • Structure of fumarate reductase from Wolinella succinogenes at 2.2A resolution. Lancaster, C. Roy D.; Kroger, Achim // Nature;11/25/1999, Vol. 402 Issue 6760, p377 

    Describes the crystal structure at 2.2A resolution of the three protein subunits containing fumarate reductase from the anaerobic bacterium Wolinella succinogenes. Structure determination; Fumarate reductase architecture; Protein subunits and prosthetic groups; Chemistry of fumarate reduction;...

  • Artificial Self-Sufficient P450 in Reversed Micelles. Hirakawa, Hidehiko; Kamiya, Noriho; Kawarabayasi, Yutaka; Nagamune, Teruyuki // Molecules;May2010, Vol. 15 Issue 5, p2935 

    Cytochrome P450s are heme-containing monooxygenases that require electron transfer proteins for their catalytic activities. They prefer hydrophobic compounds as substrates and it is, therefore, desirable to perform their reactions in non-aqueous media. Reversed micelles can stably encapsulate...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics