Phenotype analysis by MUC2, MUC5AC, MUC6, and CD10 expression in Epstein-Barr virus-associated gastric carcinoma

Barua, Rita Rani; Uozaki, Hiroshi; Chong, Ja-Mun; Ushiku, Tetsuo; Hino, Rumi; Chang, Moon-Sung; Nagai, Hideo; Fukayama, Masashi
August 2006
Journal of Gastroenterology;Aug2006, Vol. 41 Issue 8, p733
Academic Journal
Gastric marker mucins (MUC5AC and MUC6) and intestinal marker molecules (MUC2 and CD10) have been used to determine the cell lineage of epithelial cell of gastric carcinoma (GC). To clarify the characteristics of Epstein-Barr virus (EBV)-associated GC, 18 cases were immunohistochemically evaluated along with 56 cases of EBV-negative GC. MUC2 expression was lower in EBV-associated GC: immunostaining grades 0, 1, 2, 3, and 4 were observed in 10, 6, 1, 1, and 0 cases of EBV-associated GC, respectively, and in 18, 11, 15, 6, and 6 cases of EBV-negative GC, respectively ( P = 0.013). CD10 positivity (grades 2–4) in EBV-associated GC was 6%, significantly lower than in EBV-negative GC (34%) ( P = 0.030). When phenotypes of GC were categorized by the combined positivities of gastric markers (either MUC5AC or MUC6) and intestinal markers (either MUC2 or CD10), EBV-associated GC included primarily null (44%) and gastric (39%) types, but EBV-negative GC comprised null (7%), gastric (30%), intestinal (27%), and mixed (36%) types. The age of patients with gastric types was significantly younger for both EBV-associated GC and EBV-negative GC cases. Neoplastic epithelial cells of EBV-associated GC did not express MUC2 or CD10, and most of them were categorized as null or gastric types. EBV infection may occur in the epithelial cells of null or gastric phenotypes, which may be devoid of transdifferentiation potential toward intestinal phenotypes.


Related Articles

  • Epstein-Barr Virus (EBV) Infection in Epithelial Cells In Vivo: Rare Detection of EBV Replication in Tongue Mucosa but Not in Salivary Glands. Frangou, Phroso; Buettner, Maike; Niedobitek, Gerald // Journal of Infectious Diseases;1/15/2005, Vol. 191 Issue 2, p238 

    Epstein-Barr virus (EBV) is transmitted through saliva, but the cellular source is controversial. Putative reservoirs include oral epithelium and salivary glands. Tongue mucosal samples, salivary glands, and tongue carcinomas were studied, by immunohistochemistry and in situ hybridization, for...

  • Epstein-Barr Virus Infection Is Common in Inflamed Gastrointestinal Mucosa. Ryan, Julie; Shen, You-Jun; Morgan, Douglas; Thorne, Leigh; Kenney, Shannon; Dominguez, Ricardo; Gulley, Margaret // Digestive Diseases & Sciences;Jul2012, Vol. 57 Issue 7, p1887 

    Background and Aims: Epstein-Barr virus (EBV) is present in the malignant epithelial cells of 10% of all gastric adenocarcinomas; however, localization of the virus in normal gastrointestinal mucosa is largely unexplored. In the present study, we measured EBV DNA and localized viral gene...

  • Distribution and phenotype of Epstein-Barr virus-infected cells in human pharyngeal tonsils. Hudnall, S. David; Ge, Yimin; Wei, Longxing; Yang, Ning-Ping; Wang, Hui-Quin; Chen, Tiansheng // Modern Pathology;Apr2005, Vol. 18 Issue 4, p519 

    Although Epstein-Barr virus (EBV) is often found in human tonsils, it remains to be precisely determined in what cells and microenvironment the virus is present. Although generally regarded as a B lymphotropic virus, EBV is associated with non-B-cell tumors, for example, NK/T-cell lymphoma,...

  • Epstein-Barr Virus latent membrane protein 1 induces Snail and epithelial-mesenchymal transition in metastatic nasopharyngeal carcinoma. Horikawa, T.; Yoshizaki, T.; Kondo, S.; Furukawa, M.; Kaizaki, Y.; Pagano, J. S. // British Journal of Cancer;3/29/2011, Vol. 104 Issue 7, p1160 

    Background: Epstein-Barr Virus (EBV)-associated nasopharyngeal carcinoma (NPC) is distinctive among head-and-neck cancers in its undifferentiated histopathology and highly metastatic character. We have recently investigated the involvement of epithelial-mesenchymal transition (EMT)...

  • Epstein-Barr virus infection of polarized tongue and nasopharyngeal epithelial cells. Tugizov, Sharof M.; Berline, Jennifer W.; Palefsky, Joel M. // Nature Medicine;Mar2003, Vol. 9 Issue 3, p307 

    Epstein-Barr virus (EBV) initially enters the body through the oropharyngeal mucosa and subsequently infects B lymphocytes through their CD21 (CR2) complement receptor. Mechanisms of EBV entry into and release from epithelial cells are poorly understood. To study EBV infection in mucosal...

  • Activation of the cJun N-terminal kinase (JNK) pathway by the Epstein-Barr virus-encoded latent membrane protein 1 (LMP1). Eliopoulos, Aristides G; Young, Lawrence S // Oncogene;4/2/98, Vol. 16 Issue 13, p1731 

    Expression of the oncogenic Epstein-Barr virus (EBV)-encoded Latent Membrane Protein 1 (LMP1) activates signalling on the NF-κB axis through two distinct domains in the cytoplasmic C-terminus of the protein, namely CTAR1 (aa 187–231) and CTAR2 (aa 351–386). Whilst this effect is...

  • Characterization of Epstein Barr Virus Latency Pattern in Argentine Breast Carcinoma. Lorenzetti, Mario A.; De Matteo, Elena; Gass, Hugo; Vazquez, Paula Martinez; Julia Lara; Gonzalez, Pedro; Preciado, María Victoria; Chabay, Paola A. // PLoS ONE;2010, Vol. 5 Issue 10, p1 

    Introduction: Epstein-Barr virus (EBV)-associated tumors show different expression patterns of latency genes. Since in breast carcinoma this pattern is not yet fully described, our aim was to characterize EBV latency pattern in our EBV positive breast carcinoma series. Methods: The study was...

  • Epstein—Barr virus facilitates the malignant potential of immortalized epithelial cells: from latent genome to viral production and maintenance. Jian-Hong Lu; Yun-Lian Tang; Hai-Bo Yu; Jian-Hua Zhou; Chun-Yan Fu; Xi Zeng; Zheng-Yuan Yu; Hong-Ling Yin; Ming-Hua Wu; Jun-Yi Zhang; Xiao-Ling Li; Gui-Yuan Li // Laboratory Investigation (00236837);Feb2010, Vol. 90 Issue 2, p196 

    Epstein–Barr virus (EBV) is closely associated with several malignancies, including nasopharyngeal carcinoma. To investigate the EBV activity in tumor development, we tried to establish a malignant model of EBV-infected cells in nude mice. On the basis of the Maxi-EBV system, a human...

  • Establishment of a monkey kidney epithelial cell line with the BARF1 open reading frame from Epstein-Barr Virus. Wei, Ming Xin; de Turenne-Tessier, Mireille; Decaussin, Gisèle; Benet, Gérard; Ooka, Tadamasa // Oncogene;6/26/97, Vol. 14 Issue 25, p3073 

    We previously reported that the BARF1 (BamH1-A right frame 1) gene product from Epstein-Barr Virus (EBV) may have oncogenic properties since injection into new-born rats of transfected cell lines resulted in the development of BARF1 expressing tumors, which were agressive in the case of murine...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics