TITLE

Identification of Proteins Immunologically Related to Interferon Regulatory Factor-1 That Bind with Interferon Regulatory Factor Element

AUTHOR(S)
Contente, Sara; Attard, Frank A.; Friedman, Robert M.
PUB. DATE
September 2006
SOURCE
Journal of Infectious Diseases;9/16/2006, Vol. 194, pS27
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Interferon regulatory factor (IRF)-1 expression was surveyed in nontransformed and oncogene-transformed mouse fibroblasts, using Western immunoblot with an IRF-1-specific antiserum, to examine possible differences resulting from cellular transformation. Ten additional proteins that reacted with the IRF-1 antibody and that underwent specific competition by peptide antigen were observed in extracts of both nontransformed and oncogene-transformed cell lines. Cross-reacting proteins were also observed in mouse macrophage extracts. Protein was captured from fibroblast nuclear extracts, using oligonucleotides representing IRF-binding sequences linked to magnetic beads. Captured proteins were eluted and analyzed by immunoblot with anti-IRF-1. Along with 43-kDa IRF-1, 4 of the 7 nuclearly located cross-reacting proteins (97, 90, 66, and 33 kDa) were found to complex with the IRF binding element. These proteins, with an epitope in common with the IRF-1 C-terminal region and IRF element DNA sequence-binding capability, may represent new members of the IRF family.
ACCESSION #
22316926

 

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