TITLE

Ultrastructural Study of Autologous Cultivated Conjunctival Epithelium

AUTHOR(S)
Alfano, Carmine; Chiummariello, Stefano; Fioramonti, Paolo; Innocenzi, Daniele; Scuderi, Nicolo
PUB. DATE
September 2006
SOURCE
Ophthalmic Surgery, Lasers & Imaging;Sep/Oct2006, Vol. 37 Issue 5, p378
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
BACKGROUND AND OBJECTIVE: This report is one of the first in the literature on the attempted cultivation and clinical application of human conjunctival epithelium. The authors investigated the possibility of restoring severely damaged ocular surface with autologous cultivated conjunctival epithelium. PATIENTS AND METHODS: The conjunctival cells needed for the experiment were harvested from six patients with oculopalpebral diseases. Confluent epithelial sheets were developed from each biopsy specimen. The new epithelium was then implanted on the patients' or donors' eye surface. RESULTS: The histologic examination showed a pluristratified squamous non-keratinized epithelium lying on a basement membrane and with a lamina propria of well-vascularized connective tissue. Normal ultrastructural characteristics were evident on electron microscopy. CONCLUSION: The cultivation of autologous conjunctival cells may be a good option for rapid and safe repair of large single or bilateral conjunctival defects, as an alternative to heterotopic or allogenic grafts.
ACCESSION #
22274608

 

Related Articles

  • Immunolocalisation of E-cadherin and ß-catenin in human pterygium. Kase, Satoru; Osaki, Mitsuhiko; Sato, Izuru; Takahashi, Shuji; Nakanishi, Katsuya; Yoshida, Kazuhiko; Ito, Hisao; Ohno, Shigeaki // British Journal of Ophthalmology;Sep2007, Vol. 91 Issue 9, p1209 

    Aim: The pterygium represents an invasion of a wing of altered ocular surface tissue into the normal cornea. The head itself is slightly elevated and white, which is the only site of firm adhesion to the globe. The mechanisms of cell proliferation and adhesion in pterygium epithelium, however,...

  • Morphological characteristics of conjunctival squamous papillomas in relation to human papillomavirus infection. Mlakar, Jernej; Kocjan, Boštjan J; Hošnjak, Lea; Pižem, Jože; Beltram, Matej; Gale, Nina; Drnovšek-Olup, Brigita; Poljak, Mario // British Journal of Ophthalmology;Mar2015, Vol. 99 Issue 3, p431 

    Objective To determine the prevalence of a broad spectrum of human papillomavirus (HPV) types in conjunctival papillomas and a possible difference in clinical and histopathological presentation of HPVpositive and HPV-negative papillomas. Methods Formalin-fixed, paraffin-embedded papilloma tissue...

  • An unusual case of Epstein–Barr virus driven lymphoproliferative disorder of the conjunctiva which mimicked a high grade lymphoma: a sheep in wolf’s clothing. M Powari // Journal of Clinical Pathology;Jul2009, Vol. 62 Issue 7, p656 

    Lymphoid proliferations represent 25–33% of acquired sub-epithelial conjunctival lesions which are excised or biopsied in patients over 15 years of age. These lesions are reported in association with Epstein–Barr virus (EBV). One such case of EBV associated spontaneously regressed...

  • Stomatologic Aspects of Cutaneous Diseases. Gorlin, Robert J.; Sedano, Heddie O. // Journal of Dermatologic Surgery & Oncology;Jul78, Vol. 4 Issue 7, p504 

    This article discusses about stomatologic aspects of cutaneous diseases. Benign mucous membrane pemohigoid is an autoimmune disorder characterized by formation of subepithelial bullae on mucous membranes, especially the oral mucosa and the conjunctiva. Occasionally lesions are seen on the skin....

  • Adenosquamous carcinoma of the conjunctiva: A case report. SATORU KASE; HIROSHI YOSHIKAWA; YUTAKA NAKAJIMA; MIKA NODA; SUSUMU ISHIDA // Oncology Letters;2014, Vol. 7 Issue 6, p1941 

    Adenosquamous carcinoma (ASC) is a rare form of malignancy which consists of two types of cell, including squamous cells and glandular-like cells. The current report presents the first known case of ASC in the conjunctiva and analyzes the histological findings. A 76-year-old female presented...

  • Squamous cell papilloma of the conjunctiva due to human papillomavirus (HPV): presentation of two cases and review of literature. Kalogeropoulos, Chris; Koumpoulis, Ioannis; Papadiotis, Evangelos; Zioga, Aikaterini; Gkrepi, Konstantina; Pappa, Chrisavgi; Paschides, Constantinos; Malamou-Mitsi, Vasiliki; Aspiotis, Miltiadis // Clinical Ophthalmology;2012 Part 2, Vol. 6, p1553 

    Purpose: We describe two patients with squamous cell papilloma of the conjunctiva due to human papilloma virus (HPV) and review the literature. Patients and methods: Two patients with conjunctival tumors were examined and treated in the University Eye Clinic and diagnosed in the University...

  • FDA Approval for Epi-Lift.  // Review of Ophthalmology;Oct2004, Vol. 11 Issue 10, p13 

    The article focuses on the EpiLift system, the ophthalmic surgical device designed for separating corneal tissue during Epi-LASIK, that received Food and Drug Administration's approval recently. LASIK. During Epi-LASIK, the top layer of the epithelium is separated into an intact sheet of viable...

  • Esophageal epithelial surface in patients with gastroesophageal reflux disease: An electron microscopic study. Azumi, Takane; Adachi, Kyoichi; Furuta, Kenji; Nakata, Shuji; Ohara, Shunji; Koshino, Kenji; Miki, Masaharu; Morita, Terumi; Tanimura, Takashi; Ashizawa, Nobuo; Kinoshita, Yoshikazu; De Hertogh, Gert // World Journal of Gastroenterology;10/7/2008, Vol. 14 Issue 37, p5712 

    AIM: To investigate the intercellular spaces between the most superficially located esophageal epithelial cells in patients with gastroesophageal reflux disease (GERD). METHODS: Eighteen patients with erosive esophagitis, 10 patients with non-erosive reflux disease (NERD), and 18 normal...

  • A Ball of Cotton Wool Masquerading as a Conjunctiva Mass. Komolafe, O. O.; Omolade, C. O. // Nigerian Medical Journal;2011, Vol. 52 Issue 1, p66 

    The article reports on an unusual form of ocular body in a 3 year-old girl who presented clinically with a right conjunctiva mass in the right eye of 3 weeks duration. The patient reported associated recurrent discharge and occasional periorbital swelling but she had no previous trauma or...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics