TITLE

Antidiabetic thiazolidinediones inhibit invasiveness of pancreatic cancer cells via PPARc independent mechanisms

AUTHOR(S)
Galli, A.; Ceni, E.; Crabb, D. W.; Mello, T.; Salzano, R.; Grappone, C.; Milani, S.; Surrenti, E.; Surrenti, C.; Casini, A.
PUB. DATE
November 2004
SOURCE
Gut;Nov2004, Vol. 53 Issue 11, p1688
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background/Aims: Thiazolidinediones (TZD) are a new class of oral antidiabetic drugs that have been shown to inhibit growth of some epithelial cancer cells. Although TZD were found to be ligands for peroxisome proliferators activated receptor γ (PPARγ) the mechanism by which TZD exert their anticancer effect is currently unclear. Furthermore, the effect of TZD on local motility and metastatic potential of cancer cells is unknown. The authors analysed the effects of two TZD, rosiglitazone and pioglitazone, on invasiveness of human pancreatic carcinoma cell lines in order to evaluate the potential therapeutic use of these drugs in pancreatic adenocarcinoma. Methods: Expression of PPARγ in human pancreatic adenocarcinomas and pancreatic carcinoma cell lines was measured by reverse transcription polymerase chain reaction and confirmed by western blot analysis. PPARγ activity was evaluated by transient reporter gene assay. Invasion assay was performed in modified Boyden chambers. Gelatinolytic and fibrinolytic activity were evaluated by gel zymography. Results: TZD inhibited pancreatic cancer cells' invasiveness, affecting gelatinolytic and fibrinolytic activity with a mechanism independent of PPARγ activation and involving MMP-2 and PAI-1 expression. Conclusion: TZD treatment in pancreatic cancer cells has potent inhibitory effects on growth and invasiveness suggesting that these drugs may have application for prevention and treatment of pancreatic cancer in humans.
ACCESSION #
22269088

 

Related Articles

  • Suppression of pancreatic carcinoma growth by activating peroxisome proliferator-activated receptor γ involves angiogenesis inhibition. Yu-Wei Dong; Xing-Peng Wang; Kai Wu // World Journal of Gastroenterology;1/28/2009, Vol. 15 Issue 4, p441 

    AIM: To study the possible actions and mechanisms of peroxisome proliferator-activated receptor γ (PPARγ), a ligand-activated transcription factor, in pancreatic carcinogenesis, especially in angiogenesis. METHODS: Expressions of PPARγ and retinoid acid receptor (RXRα) were examined...

  • Tumor-Suppressor Function of SPARC-Like Protein 1/Hevin in Pancreatic Cancer. Esposito, Irene; Kayed, Hany; Keleg, Shereen; Giese, Thomas; Sage, E. Helene; Schirmacher, Peter; Friess, Helmut; Kleeff, Jörg // Neoplasia;Jan2007, Vol. 9 Issue 1, p8 

    SPARC-like protein 1 (SPARCL1), a member of the SPARC family, is downregulated in various tumors. In the present study, the expression and localization of SPARCL1 were analyzed in a wide range of nontumorous and neoplastic pancreatic tissues by quantitative reverse transcription-polymerase chain...

  • Role of RHOT1 on migration and proliferation of pancreatic cancer. Qingqing Li; Lei Yao; Youzhen Wei; Shasha Geng; Chengzhi He; Hua Jiang // American Journal of Cancer Research;2015, Vol. 5 Issue 4, p1460 

    Pancreatic cancer (PC) is one of the most malignant tumors. Rho GTPases can affect several types of human cancers, including PC. In this study, we investigated the role of Ras homolog family member T1 (RHOT1), a new member of Rho GTPases in PC. IHC results showed that RHOT1 was expressed...

  • Genome-Wide Analysis in Human Colorectal Cancer Cells Reveals Ischemia-Mediated Expression of Motility Genes via DNA Hypomethylation. Skowronki, Karolina; Andrews, Joseph; Rodenhiser, David I.; Coomber, Brenda L. // PLoS ONE;Jul2014, Vol. 9 Issue 7, p1 

    DNA hypomethylation is an important epigenetic modification found to occur in many different cancer types, leading to the upregulation of previously silenced genes and loss of genomic stability. We previously demonstrated that hypoxia and hypoglycaemia (ischemia), two common micro-environmental...

  • Silibinin inhibits prostate cancer invasion, motility and migration by suppressing vimentin and MMP-2 expression. Kai-jie WU; Jin ZENG; Guo-dong ZHU; Lin-lin ZHANG; Dong ZHANG; Lei LI; Jin-hai FAN; Xin-yang WANG; Da-lin HE // Acta Pharmacologica Sinica;Aug2009, Vol. 30 Issue 8, p1162 

    AbstractAim:Silibinin is known to exert growth inhibition and cell death together with cell cycle arrest and apoptosis in human prostate cancer cells. Whether silibinin could inhibit the invasion, motility and migration of prostate cancer cells remains largely unknown. This study was designed to...

  • Nischarin Is Differentially Expressed in Rat Brain and Regulates Neuronal Migration. Yuemin Ding; Ruyi Zhang; Kena Zhang; Xinyou Lv; Yanan Chen; Aiqing Li; Linlin Wang; Xiong Zhang; Qiang Xia // PLoS ONE;Jan2013, Vol. 8 Issue 1, Special section p1 

    Nischarin is a protein known to inhibit breast cancer cell motility by regulating the signaling of the Rho GTPase family. However, little is known about its location and function in the nervous system. The aim of the present study was to investigate the regional and cellular expression and...

  • Nanog and Oct4 overexpression increases motility and transmigration of melanoma cells. Borrull, Aurelie; Ghislin, Stephanie; Deshayes, Frederique; Lauriol, Jessica; Alcaide-Loridan, Catherine; Middendorp, Sandrine // Journal of Cancer Research & Clinical Oncology;Jul2012, Vol. 138 Issue 7, p1145 

    Purpose: Melanoma tumors are highly heterogeneous and can undergo phenotypic modifications depending on their plasticity and the microenvironment, with shifts between proliferative and invasive states. We have shown that melanoma cells, grown as spheroids in a neural crest cell medium, polarize...

  • Midkine mRNA Is Overexpressed in Pancreatic Cancer. Seiji Ohhashi; Takuya Egami; Jun Yu; Lin Cui; Hiroki Toma; Shunichi Takahata; Toshinaga Nabae; Masao Tanaka // Digestive Diseases & Sciences;Apr2009, Vol. 54 Issue 4, p811 

    Abstract   Purpose Midkine (MK) has been reported to be a possible molecular marker for the diagnosis of pancreatic cancer. We investigated the feasibility of quantitative analysis of MK mRNA by quantitative real-time RT-PCR (qRT-PCR) as a promising tool for the diagnosis of pancreatic...

  • Gene Expression Analysis In Gemcitabine Resistant Cells Derived From Human Pancreatic Cancer Cell. Shaoxuan Zhang; Yarong Li; Tatsuhiko Furugawa; Homare Takahashi; Xiaofang Che; Hongye Zhao; Sinichi Akiyama // Journal of Convergence Information Technology;Dec2011, Vol. 6 Issue 12, p460 

    To analyze the expression of genes involved in gemcitabine resistance formation in gemcitabineresistant cells derived from human pancreatic cancer cell, we established several cell lines with different acquired resistance to gemcitabine from pancreatic cancer cell line MIA-PaCa2, and analyzed...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics