TITLE

Helicobacter pyloric outer membrane proteins and gastroduodenal disease

AUTHOR(S)
Yamaoka, Y.; Ojo, O.; Fujimoto, S.; Odenbreit, S.; Haas, R.; Gutierrez, O.; El-Zimaity, H. M. T.; Reddy, R.; Arnqvist, A.; Graham, D. Y.
PUB. DATE
June 2006
SOURCE
Gut;Jun2006, Vol. 55 Issue 6, p775
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background and aims: A number of Helicobacter pylori outer membrane proteins (OMPs) undergo phase variations. This study examined the relation between OMP phase variations and clinical outcome. Methods: Expression of H pylori BabA, BabB, SabA, and OipA proteins was determined by immunoblot. Multiple regression analysis was performed to determine the relation among OMP expression, clinical outcome, and mucosal histology. Results: H pylori were cultured from 200 patients (80 with gastritis, 80 with duodenal ulcer (DU), and 40 with gastric cancer). The most reliable results were obtained using cultures from single colonies of low passage number. Stability of expression with passage varied with OipA > BabA > BabB > SabA. OipA positive status was significantly associated with the presence of DU and gastric cancer, high H pylori density, and severe neutrophil infiltration. SabA positive status was associated with gastric cancer, intestinal metaplasia, and corpus atrophy, and negatively associated with DU and neutrophil infiltration. The Sydney system underestimated the prevalence of intestinal metaplasia/atrophy compared with systems using proximal and distal corpus biopsies. SabA expression dramatically decreased following exposure of H pylori to pH 5.0 for two hours. Conclusions: SabA expression frequently switched on or off, suggesting that SabA expression can rapidly respond to changing conditions in the stomach or in different regions of the stomach. SabA positive status was inversely related to the ability of the stomach to secrete acid, suggesting that its expression may be regulated by changes in acid secretion and/or in antigens expressed by the atrophic mucosa.
ACCESSION #
22212740

 

Related Articles

  • Helicobacter pylori Omp18 and Its Application in Serologic Screening of Infection. Talebkhan, Yeganeh; Ebrahimzadeh, Fatemeh; Esmaeili, Maryam; Zamaninia, Leili; Nahvijoo, Azin; Khedmat, Hossein; Fereidooni, Forouzandeh; Mohagheghi, Mohammad Ali; Mohammadi, Marjan // Current Microbiology;Jan2011, Vol. 62 Issue 1, p325 

    Helicobacter pylori (Hp) is a major risk factor for gastrointestinal disorders including gastric cancer. We evaluated host serum antibody responses toward outer membrane protein18 in comparison with Urease A and B subunits. omp18 and ureA- ureB gene fragments were PCR amplified, cloned, and...

  • Association of Helicobacter pylori Infection With Gastric Carcinoma: A Meta-Analysis. Eslick, Guy D.; Lim, Lunette L.-Y.; Byles, Julie E.; Xia, Harry H-X.; Talley, Nicholas J. // American Journal of Gastroenterology;Sep1999, Vol. 94 Issue 9, p2373 

    OBJECTIVE: As conflicting studies have recently been published, we aimed to determine if Helicobacter pylori (H. pylori) infection is associated with gastric adenocarcinoma. METHODS: This was a meta-analysis of observational epidemiological studies. RESULTS: A total of 42 studies met...

  • Ulcer Microbe Alters Itself To Dodge Immune Attack.  // Nutrition Health Review: The Consumer's Medical Journal;2014, Issue 111, p7 

    The article offers information on Helicobacter (H.) pylori, a stomach ulcer-causing bacterium which can change itself to avoid immune response of the body.

  • Decrease in Ornithine Decarboxylase Activity after Eradication of Helicobacter pylori. Alam, Khalid; Arlow, Freda L.; Ma, Chan K.; Schubert, Timothy T. // American Journal of Gastroenterology;Jun1994, Vol. 89 Issue 6, p888 

    Objective: Our aim was to determine whether gastric mucosal ODC activity is altered after successful eradication of HP. Recent reports have suggested that Helicobacter pylori (HP) infection of the stomach is associated with the development of gastric cancer. Gastrointestinal cancers usually do...

  • Caveolin-1 Protects B6129 Mice against Helicobacter pylori Gastritis. Hitkova, Ivana; Gang Yuan; Anderl, Florian; Gerhard, Markus; Kirchner, Thomas; Reu, Simone; Röcken, Christoph; Schäfer, Claus; Schmid, Roland M.; Vogelmann, Roger; Ebert, Matthias P. A.; Burgermeister, Elke // PLoS Pathogens;Apr2013, Vol. 9 Issue 4, Special section p1 

    Caveolin-1 (Cav1) is a scaffold protein and pathogen receptor in the mucosa of the gastrointestinal tract. Chronic infection of gastric epithelial cells by Helicobacter pylori (H. pylori) is a major risk factor for human gastric cancer (GC) where Cav1 is frequently down-regulated. However, the...

  • Levels of Interleukin-18 Are Markedly Increased in Helicobacter pylori-Infected Gastric Mucosa among Patients with Specific IL18 Genotypes. Sakai, Kyoko; Kita, Masakazu; Sawai, Naoki; Shiomi, Satoshi; Yoshio Sumida; Kanemasa, Kazuyuki; Mitsufuji, Shoji; Imanishi, Jiro; Yamaoka, Yoshio // Journal of Infectious Diseases;6/15/2008, Vol. 197 Issue 12, p1752 

    Background. The cellular immune response in gastric mucosa infected with Helicobacter pylori is proposed to be predominantly of the T helper cell type 1 type. Methods. Interleukin (IL)-18, IL-12, and interferon (IFN)-γ levels were measured in gastric mucosal biopsy specimens by...

  • Helicobacter Pylori and Refractory Duodenal Ulcers: Cross-over Comparison of Continued Cimetidine with Cimetidine plus Antimicrobials. Boyd, H. K.; Zaterka, S.; Eisig, J. N.; Chinzon, D.; Iriya, K.; Laudanna, A. A.; Boyd, E. J. S. // American Journal of Gastroenterology;Sep1994, Vol. 89 Issue 9, p1505 

    Objectives: To determine the distribution of Helicobacter pylori in the antral and duodenal mucosa of patients with duodenal ulcers refractory to 12 wk of treatment with cimetidine and to evaluate the effect of adding antimicrobial agents to cimetidine on the healing of refractory duodenal...

  • Gastroduodenal Mucus Gel Thickness in Patients with Helicobacter pylori: A Method for Assessment of Biopsy Specimens. Sarosiek, J.; Marshall, B. J.; Peura, D. A.; Hoffman, S.; Feng, T.; McCallum, R. W. // American Journal of Gastroenterology;Jun1991, Vol. 86 Issue 6, p729 

    The gastroduodenal mucus layer is considered the primary mucosal protective barrier, especially important in the maintenance of a mucosal pH gradient. Thus, the measurement of the mucus layer thickness in various disease states could advance our understanding of gastroduodenal pathophysiology....

  • Enhanced Mucosal Interleukin-6 and -8 in Helicobacter pylori- Positive Dyspeptic Patients. Gionchetti, Paolo; Vaira, Dino; Campieri, Massimo; Holton, John; Menegatti, Marcello; Belluzzi, Andrea; Bertinelli, Elisabetta; Ferretti, Maurizio; Brignola, Corrado; Miglioli, Mario; Barbara, Luigi // American Journal of Gastroenterology;Jun1994, Vol. 89 Issue 6, p883 

    Objectives: To determine the concentrations of interleukin-1β, interleukin-6, and interleukin-8 in tissue homogenates of mucosal biopsy specimens from Helicobacter bacter pylori-positive and -negative patients. Methods: In 43 consecutive patients who underwent upper gastrointestinal...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics