TITLE

DNA FINGERPRINTING ELICITED EVOLUTIONARY TREND OF ORAL CANDIDA TROPICALIS ISOLATES FROM DIVERSE GEOGRAPHIC LOCALES

AUTHOR(S)
Dassanayake, R. S.; Samaranayake, Y. H.; Yau, J. Y. Y.; Samaranayake, L. P.
PUB. DATE
July 2006
SOURCE
Indian Journal of Medical Microbiology;Jul2006, Vol. 24 Issue 3, p186
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: To study molecular profiles of oral Candida tropicalis isolates from five different geographic locales to determine the molecular diversity, clonality and evolutionary trends of this opportunistic pathogen. Methods: A total of 36 strains from five countries (China, Canada, Scotland, Japan and Tanzania) were genotyped by PCR fingerprinting with 11 separate primers. Of these, primers RSG9, RSG8, T3B and RSD12 generated complex fingerprinting patterns. Results: Three significantly dissimilar profiles were derived from the primer T3B and particularly focused on tDNA suggested the prevalence of genetic subtypes within the species. Comparison of tDNA and rDNA (RSD12) fingerprints of C. tropicalis suggested that rDNA is much more heterogeneous than the relatively distinct tDNA. Further analysis of similarity coefficient (SAB) of gel profiles derived from computer-generated dendrograms indicated some degree of similarity in isolates from five-disparate geographic locales as well as the presence of unique isotypes in each region. Conclusions: This study demonstrates the evolutionary divergence of distinct genetic subgroups within Candida tropicalis.
ACCESSION #
22150597

 

Related Articles

  • Caspofungin use in patients with invasive candidiasis caused by common non-albicans Candida species: review of the caspofungin database.  // Journal of Invasive Fungal Infections;2010, Vol. 4 Issue 3, p121 

    In this study, 212 patients with candidiasis caused by non-albicans Candida spp. were identified from five clinical trials of caspofungin monotherapy. The particular species involved were Candida parapsilosis, Candida tropicalis, Candida glabrata, Candida krusei, Candida guilliermondii, and...

  • Community-Acquired Fungemia Due to a Multiple-Azole-Resistant Strain of Candida tropicalis . Jandourek, Alena; Brown, Patricia; Vazquez, Jose A. // Clinical Infectious Diseases;12/1/1999, Vol. 29 Issue 6, p1583 

    Describes an incident of community-acquired Candida tropicalis fungemia where the patients remained candidemic despite high-dose fluconazole therapy. Laboratory findings; Administration of amphotericin B lipid complex; Strain delineation of the C. tropicales isolates; Importance of developing...

  • Antifungal susceptibilities of Candida glabrata species complex, Candida krusei, Candida parapsilosis species complex and Candida tropicalis causing invasive candidiasis in China: 3 year national surveillance. Xiao, Meng; Fan, Xin; Chen, Sharon C.-A.; Wang, He; Sun, Zi-Yong; Liao, Kang; Chen, Shu-Lan; Yan, Yan; Kang, Mei; Hu, Zhi-Dong; Chu, Yun-Zhuo; Hu, Tie-Shi; Ni, Yu-Xing; Zou, Gui-Ling; Kong, Fanrong; Xu, Ying-Chun // Journal of Antimicrobial Chemotherapy (JAC);Mar2015, Vol. 70 Issue 3, p802 

    Objectives To define the antifungal susceptibility patterns of the most common non-albicans Candida spp. in China. Methods We evaluated the susceptibilities to nine antifungal drugs of Candida parapsilosis species complex, Candida tropicalis, Candida glabrata species complex and Candida krusei...

  • Prosthetic Valve Endocarditis Due to Candida Tropicalis Complicated by Multiple Pseudoaneurysms. Zedtwitz-Liebenstein, K.; Gabriel, H.; Willinger, B.; Ehringer, H.; Polterauer, P.; Graninger, W. // Infection;Jun2001, Vol. 29 Issue 3, p177 

    Candida endocarditis is an unusual but severe complication caused by Candida albicans or other fungal species. We describe a case of prosthetic valve endocarditis due to Candida tropicalis, complicated by multiple pseudoaneurysms.

  • Micafungin for Invasive Candidiasis. Deresinski, Stan // Infectious Disease Alert;Nov2007, Vol. 27 Issue 2, p15 

    Patients with invasive candidiasis were randomized to blinded treatment with either caspofungin (70 mg on day one, followed by 50 mg daily), or one of 2 doses of micafungin (100 mg or 150 mg daily). Approximately 85% of the 595 randomized patients had candidemia. Patients in each treatment arm...

  • Renal Candida tropicalis abscesses in a patient with acute lymphoblastic leukemia. Li, W.-Y.; Wu, V.-C.; Lin, W.-C.; Chen, Y.-M. // Kidney International;Aug2007, Vol. 72 Issue 3, p382 

    The article presents a case study involving a 17-year-old man with acute lymphoblastic leukemia associated with renal candida tropicalis abscesses. Several test including repeated abdominal echo and computed tomography revealed microabscesses in the spleen and bilateral kidneys. The patient has...

  • Assessment of DNA fingerprinting for rapid identification of outbreaks of systemic candidiasis. Matthews, Ruth; Burnie, James // BMJ: British Medical Journal (International Edition);2/11/89, Vol. 298 Issue 6670, p354 

    Assesses DNA fingerprinting for rapid identification of outbreaks of systematic candidiasis. Production of different genotypes; Comparison of DNA fingerprinting with other typing systems; Benefits of DNA fingerprinting.

  • Overview of Opportunistic Fungal Infections in India. Chakrabarti, Arunaloke; Chatterjee, Shiv Sekhar; Shivaprakash, M. R. // Japanese Journal of Medical Mycology;Sep2008, Vol. 49 Issue 3, p165 

    In recent years fungi have been flourishing in immunocompromised patients of tertiary care centers. The data on the burden of opportunistic mycoses in India is not clear though the climate in this country is well suited for a wide variety of fungal infections. There are very few good diagnostic...

  • Histopathological Characteristics of Experimental Candida tropicalis Induced Acute Systemic Candidiasis in BALB/c Mice. Chen, Yong Voon; Rosli, Rozita; Fong, Seow Heng; Sidik, Shiran Mohd; Pei, Chong Pei // International Journal of Zoological Research;2012, Vol. 8 Issue 1, p12 

    Systemic candidiasis caused by Candida tropicalis is potentially fatal in human but studies relating the histological characteristics with C. tropicalis induced acute systemic candidiasis are few in number. Hence, this study was undertaken to establish and to characterize the distinctive...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics