C and N stable isotope variation in urine and milk of cattle depending on the diet

Knobbe, N.; Vogl, J.; Pritzkow, W.; Panne, U.; Fry, H.; Lochotzke, H. M.; Preiss-Weigert, A.
October 2006
Analytical & Bioanalytical Chemistry;Oct2006, Vol. 386 Issue 1, p104
Academic Journal
The stable carbon and nitrogen isotopic composition of urine and milk samples from cattle under different feeding regimes were analysed over a period of six months. The isotope ratios were measured with isotope ratio mass spectrometry (IRMS). The d 13C values of milk and urine were dependent on different feeding regimes based on C3 or C4 plants. The d 13C values are more negative under grass feeding than under maize feeding. The d 13C values of milk are more negative compared to urine and independent of the feeding regime. Under grass feeding the analysed milk and urine samples are enriched in 13C relative to the feed, whereas under maize feeding the 13C/12C ratio of urine is in the same range and milk is depleted in 13C relative to the diet. The difference between the 15N/14N ratios for the two feeding regimes is less pronounced than the 13C/12C ratios. The d 15N values in urine require more time to reach the new equilibrium, whereas the milk samples show no significant differences between the two feeding regimes.


Related Articles

  • Measurements of stable isotope ratios in milk samples from a farm placed in the mountains of Transylvania. Magdas, D. A.; Cristea, G.; Cordea, D. V.; Bot, A.; Puscas, R.; Radu, S.; Mirel, V.; Mihaiu, M. // AIP Conference Proceedings;Nov2013, Vol. 1565 Issue 1, p304 

    Product origin is of great importance for consumers especially because its association in consumer's perception with food quality, freedom from disease or pollution. Stable isotope ratio analysis is a powerful technique in food authenticity and traceability control which has been introduced...

  • Determination of tenuazonic acid in human urine by means of a stable isotope dilution assay. Asam, Stefan; Habler, Katharina; Rychlik, Michael // Analytical & Bioanalytical Chemistry;May2013, Vol. 405 Issue 12, p4149 

    The content of tenuazonic acid in human urine was determined by a stable isotope dilution assay (SIDA) that was recently developed for the analysis of food commodities and extensively re-validated for urine matrix in this study. Linearity of the response curve was proven between molar ratios...

  • Abstractions.  // Nature;9/25/2008, Vol. 455 Issue 7212, pxi 

    The article provides information related to milk. Cattle, sheep, and goats were domesticated more than 10,000 years ago. Richard Evershed, a biogeochemist at the University of Bristol, invented a stable isotope technique to detect milk residues and has used it to analyze an unprecedented...

  • Stable isotopes and faunal bones. Comments on Milner et al. (2004). Barberena, R.; Borrero, L. A. // Antiquity;Mar2005, Vol. 79 Issue 303, p191 

    The authors comment on ways of comparing the results of stable isotopes, on the one hand, and faunal remains, on the other, as evidence for diet.

  • Touch not the fish: the Mesolithic- Neolithic change of diet and its significance. Richards, M. P.; Schulting, R. J. // Antiquity;Jun2006, Vol. 80 Issue 308, p444 

    Stable isotope analysis has startled the archaeological community by showing a rapid and widespread change from a marine to terrestrial diet (ie from fish to domesticated plants and animals) as people moved from a Mesolithic to a Neolithic culture. This could be a consequence of domestication,...

  • 12. Zinc: 12.2 Zinc metabolism and homeostasis.  // Vitamin & Mineral Requirements in Human Nutrition;2004, p231 

    Chapter 12.2 of the book "Vitamin & Mineral Requirements in Human Nutrition" is presented. It explores zinc metabolism and homeostasis. It states that zinc absorption is concentration dependent and occurs throughout the small intestine. According to the article, the introduction of stable...

  • Sources of variation in consumer-diet δ15N enrichment: a meta-analysis. Vanderklift, Mathew A.; Ponsard, Sergine // Oecologia;Jul2003, Vol. 136 Issue 2, p169 

    Measurements of δ15N of consumers are usually higher than those of their diet. This general pattern is widely used to make inferences about trophic relationships in ecological studies, although the underlying mechanisms causing the pattern are poorly understood. However, there can be...

  • Reconstructing Meat Consumption through Biomarker Analyses of Paleofeces. Battillo, Jenna M.; Fisher, Abigail E. // Ethnobiology Letters;2015, Vol. 6 Issue 1, p111 

    This mini-review outlines three underutilized approaches for studying meat-based biomarkers in archaeological paleofeces that we expect will increase in significance within the field. Myoglobin, stable isotope, and aDNA analyses all have untapped potential to inform meat-based dietary constituents.

  • Differentiation of organically and conventionally produced milk by stable isotope and fatty acid analysis. Molkentin, Joachim; Giesemann, Anette // Analytical & Bioanalytical Chemistry;May2007, Vol. 388 Issue 1, p297 

    Increasing sales of organic milk mean intensified tests for authenticity are required. In addition to comprehensive documentation, analytical methods to identify organic milk, and thus to differentiate it from conventional milk, are needed for consumer protection. Because the composition of milk...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics