TITLE

Novel polysaccharide–protein-based amphipathic formulations

AUTHOR(S)
Bach, Horacio; Gutnick, David L.
PUB. DATE
June 2006
SOURCE
Applied Microbiology & Biotechnology;Jun2006, Vol. 71 Issue 1, p34
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Previous results showed that the cell-surface esterase from Acinetobacter venetianus RAG-1 enhances the emulsification properties of the polymeric bioemulsifier emulsan and its deproteinated derivative apoemulsan (Bach H, Berdichevsky Y, Gutnick D (2003) An exocellular protein from the oil-degrading microbe Acinetobacter venetianus RAG-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan. Appl Environ Microbiol 69:2608–2615). Here we show that in the presence of the his-tagged recombinant esterase from RAG-1, 18 different polysaccharides from microbial, plant, insect and synthetic sources formed hexadecane-in-water emulsions. Emulsifying activities were distributed over a 13-fold range from over 4800 U/mg protein/mg polysaccharide in the case of apoemulsan to 370 U/mg protein/mg polysaccharide in the case of alginic acid. The stability of the emulsions ranged between 95 and 58%. Emulsions formed in the presence of seven of the polysaccharides exhibited stabilities of over 80%. The esterase from A. calcoaceticus BD4, which shows sequence homology to the RAG-1 esterase, was inactive in emulsification enhancement. The sequence of the RAG-1 esterase was shown to contain two conserved peptide sequences previously shown to be implicated in carbohydrate/polysaccharide binding. A hypothetical model illustrating a possible mode of interaction between the esterase, the apoemulsan and the oil droplet is presented. The complex is presumed to generate a series of “coated” oil droplets which are restricted in their ability to coalesce resulting in a relatively stable emulsion.
ACCESSION #
21969214

 

Related Articles

  • Germ-Stabbing Particles.  // Technology Review;Jul/Aug2011, Vol. 114 Issue 4, p84 

    The article cites a research study by scientists at IBM Almaden Research to develop nanoparticles that would target the cell membranes of a large group of infectious agents called gram-positive bacteria. They drew on a library of previously developed polymer building blocks to make...

  • Construction of a chimeric gene cluster for the biosynthesis of apoemulsan with altered molecular weight. Dams-Kozlowska, Hanna; Sainath, Nina; Kaplan, David // Applied Microbiology & Biotechnology;Mar2008, Vol. 78 Issue 4, p677 

    Acinetobacter venetianus RAG-1 produces an extracellular protein/high-molecular-weight (HMW) polysaccharide complex termed emulsan. As an emulsion stabilizer, emulsan has potential industrial applications. To control the molecular weight of the polymer, a stable chromosomal mutant was generated...

  • Immunogenicity of Cell Wall and Plasma Membrane Components of Some Oral Lactic Acid Bacteria. WICKEN, A. J.; KNOX, K. W. // Journal of Dental Research;Apr1976 Supplement 3, Vol. 55, pC34 

    The article presents research into the importance of immunogenicity of the cell wall and plasma membrane in identification of bacteria and possible immune response in host animals. Particular attention is paid to the immunogenicity of surface polysaccharides and teichoic acids of lactobacilli as...

  • Bacterial polysaccharide co-polymerases share a common framework for control of polymer length. Tocilj, Ante; Munger, Christine; Proteau, Ariane; Morona, Renato; Purins, Leanne; Ajamian, Eunice; Wagner, John; Papadopoulos, Magdalene; Van Den Bosch, Luisa; Rubinstein, John L.; Féthière, James; Matte, Allan; Cygler, Miroslaw // Nature Structural & Molecular Biology;Feb2008, Vol. 15 Issue 2, p130 

    The chain length distribution of complex polysaccharides present on the bacterial surface is determined by polysaccharide co-polymerases (PCPs) anchored in the inner membrane. We report crystal structures of the periplasmic domains of three PCPs that impart substantially different chain length...

  • mesosome.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1451 

    A definition of the term "mesosome" is presented, which, in bacteria, refers to invaginations of the cell membrane.

  • Mollicutes.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1483 

    An encyclopedia entry for "Mollicutes," which refers to a class of very small bacteria, some of which have cell walls and others of which have no cell walls but are surrounded by a cell membrane, is presented.

  • Isolation of a Pseudomonas aeruginosa strain from soil that can degrade polyurethane diol. Mukherjee, Koushik; Tribedi, Prosun; Chowdhury, Arup; Ray, Tanusree; Joardar, Archi; Giri, Subhajit; Sil, Alok Kumar // Biodegradation;Apr2011, Vol. 22 Issue 2, p377 

    Polyurethane diol (PUR-diol), a synthetic polymer, is widely used as a modifier for water-soluble resins and emulsions in wood appliances and auto coatings. Non-biodegradability of polyurethanes (PUR) and PUR-based materials poses a threat to environment that has led scientists to isolate...

  • Exposure to chlorine affects the extracellular polymeric substance production and cell surface hydrophobicity in biofilm bacteria. Rathi, Rengaswamy; Satheesh, Sathyaneson // Oceanological & Hydrobiological Studies;2012, Vol. 41 Issue 4, p17 

    Chlorination is a common antifouling method adopted by industrial units to minimize the fouling growth on cooling systems. In the present study, the effect of sodium hypochlorite on extracellular polymeric substance (EPS) production, hydrophobicity, cell adhesion and viability of marine bacteria...

  • Extracellular DNA and Type IV pili mediate surface attachment by Acidovorax temperans. Björn Heijstra; Franz Pichler; Quanfeng Liang; Razel Blaza; Susan Turner // Antonie van Leeuwenhoek;May2009, Vol. 95 Issue 4, p343 

    Abstract  Extracellular DNA can play a structural role in the microbial environment. Here evidence is presented that an environmental isolate of Acidovorax temperans utilises extracellular DNA for intercellular and cell-surface attachment and that Type IV pili and electrostatic...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics