TITLE

Cardiomyocyte apoptosis and duration of aortic clamping in pig model of open heart surgery

AUTHOR(S)
Malmberg, Markus; Vähäsilta, Tommi; Saraste, Antti; Kytö, Ville; Kiss, Jan; Kentala, Erkki; Kallajoki, Markku; Savunen, Timo
PUB. DATE
September 2006
SOURCE
European Journal of Cardio-Thoracic Surgery;Sep2006, Vol. 30 Issue 3, p480
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract: Objective: Apoptotic cardiomyocyte death is induced during open heart surgery, but its determinants are poorly understood. Prolonged aortic clamping time is associated with adverse clinical outcomes. The purpose of this study was to determine whether occurrence of cardiomyocyte apoptosis is related to the duration of aortic clamping in experimental pig model of cardiac surgery with cardiopulmonary bypass. Methods: The pigs (mean weight 29±1kg) were randomly divided to undergo cardioplegic arrest for 60 (n =4) or 90 (n =4)min followed by reperfusion period of 120min. Control group (n =5) was connected to cardiopulmonary bypass for 120min without cardioplegic arrest. Cardiomyocyte apoptosis was detected (TUNEL assay and immunohistochemical staining of active caspase-3) in left ventricular tissue samples obtained before ischemia and after the ischemia-reperfusion period. Results: Apoptotic cardiomyocytes were found in all samples obtained after cardioplegic arrest and cardiopulmonary bypass alone with the TUNEL assay. The amount of apoptosis after the 120min of cardiopulmonary bypass alone in the control group was 0.006±0.001%. Compared with this, cardiomyocyte apoptosis was increased after cardioplegic arrest. After 60min of aortic cross-clamp the amount of apoptosis was 0.019±0.004% (p =0.031). After 90min of aortic cross-clamp the amount was 0.042±0.005% (p <0.001) being significantly higher than after 60min (p =0.001). Aortic cross-clamp of 90min also resulted in a detectable increase in caspase-3 activation when compared with controls. Conclusions: The occurrence of cardiomyocyte apoptosis increases with prolonged aortic clamping time during open heart surgery.
ACCESSION #
21920247

 

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