TITLE

Fractionation of selenium-containing proteins in serum by multiaffinity liquid chromatography before size-exclusion chromatography�ICPMS

AUTHOR(S)
Palacios, �scar; Encinar, Jorge Ruiz; Schauml�ffel, Dirk; Lobinski, Ryszard
PUB. DATE
March 2006
SOURCE
Analytical & Bioanalytical Chemistry;Mar2006, Vol. 384 Issue 6, p1276
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Immunoaffinity chromatography has been investigated for fractionation of serum into selenoalbumin and true selenoproteins. Among several albumin-depletion kits tested, a multiaffinity column specifically binding albumin and five other major serum proteins provided the best results. It extracted ca 95% of both albumin and selenoalbumin, which enabled interference-free determination of glutathione peroxidase, selenoprotein P, and selenoalbumin by size-exclusion chromatography combined with inductively coupled plasma mass spectrometry (SEC�ICPMS). The efficiency of the multiaffinity column did not vary over a period of 18 months. The purity of fractions separated by immunoaffinity LC was confirmed by elution-volume matching with standards in SEC�ICPMS and by selenopeptide mapping in capillary HPLC�ICPMS. Quantification of the selenium distribution among the different proteins in human serum from a control group and from a person on a selenium-rich diet revealed that 67% of the supplemented selenium was incorporated into albumin, 30% into glutathione peroxidase, and 3% into selenoprotein P.
ACCESSION #
21909054

 

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