A Mouse Monoclonal Antibody against Epstein-Barr Virus Envelope Glycoprotein 350 Prevents Infection Both In Vitro and In Vivo

Haque, Tanzina; Johannessen, Ingolfur; Dombagoda, Dilani; Sengupta, Chandrya; Burns, David M.; Bird, Pru; Hale, Geoff; Mieli-Vergani, Giorgina; Crawford, Dorothy H.
September 2006
Journal of Infectious Diseases;9/1/2006, Vol. 194 Issue 5, p584
Academic Journal
A mouse monoclonal antibody (MAb) against Epstein-Barr virus (EBV) envelope glycoprotein 350, 72A1, inhibited EBV infection of B lymphocytes in vitro. When severe combined immunodeficient mice were injected with EBV-seronegative donors' peripheral-blood mononuclear cells and challenged with EBV, 72A1 MAb prevented development of EBV-positive tumors: none of the test mice (0/12) developed EBV-positive tumors. In contrast, 67% (8/12) of control mice developed EBV-positive tumors (P = .001 ). Purified 72A1 MAb was infused into 1 healthy adult and 4 EBV-seronegative children after liver transplant. No adverse reactions were seen in the adult or in 3 of the transplant recipients. The remaining patient developed a hypersensitivity reaction, thus underlining the need to humanize the MAb.


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