TITLE

Bio-assay based on single molecule fluorescence detection in microfluidic channels

AUTHOR(S)
Hollars, Christopher W.; Puls, Jana; Bakajin, Olgica; Olsan, Brad; Talley, Chad E.; Lane, Stephen M.; Huser, Thomas
PUB. DATE
September 2006
SOURCE
Analytical & Bioanalytical Chemistry;Sep2006, Vol. 385 Issue 8, p1384
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A rapid bioassay is described based on the detection of colocalized fluorescent DNA probes bound to DNA targets in a pressure-driven solution flowing through a planar microfluidic channel. By employing total internal reflection excitation of the fluorescent probes and illumination of almost the entire flow channel, single fluorescent molecules can be efficiently detected leading to the rapid analysis of nearly the entire solution flowed through the device. Cross-correlation between images obtained from two spectrally distinct probes is used to determine the target concentration and efficiently reduces the number of false positives. The rapid analysis of DNA targets in the low pM range in less than a minute is demonstrated.
ACCESSION #
21857596

 

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