Cholinesterase inhibition based determination of pancuronium bromide in biological samples

Stankov-Jovanović, Vesna P.; Nikolić-Mandić, Snežana D.; Mandić, Ljuba M.; Mitić, Violeta D.
September 2006
Analytical & Bioanalytical Chemistry;Sep2006, Vol. 385 Issue 8, p1462
Academic Journal
Pancuronium bromide (PCBr) inhibition effect on enzyme cholinesterase from pooled human serum (Che, EC acylcholine acylhydrolase) was used for development of a spectrophotometric kinetic method for PCBr determination in human serum and urine. Optimal conditions for the basic and inhibitor reactions were established: pH=7.7 and substrate concentration c(benzoylcholine chloride)=1.33 mmol/L. Kinetic parameters were also determined: Michaelis-Menten’s constant KM=0.40 mmol/L, maximal reaction rate Vmax=52.2 μmol/L min, inhibition constant Ki=0,56 μmol/L and IC50=1.31 μmol/L. Linear dependence between the reaction rate and inhibitor concentration exists in PCBr concentration range 8.20–68.25 nmol/L, which corresponds to the real sample concentrations from 0.328 to 2.730 μmol/L. The method detection and quantification limits were 2.01 nmol/L and 6.67 nmol/L, respectively. Precision of the method was tested for three pancuronium concentrations (10.70, 29.35 and 51.25 nmol/L). Relative standard deviation (RSD) was in the range 0.15–7.45%. Accuracy was examined by standard addition method. Influence of the substances usually present in serum and urine on the reaction rate was tested. The developed method was applied for PCBr content determination in serum model samples, urine model samples and in urine taken during surgery. The method has good sensitivity, accuracy, precision and it is suitable for clinical practice. [Figure not available: see fulltext.]


Related Articles

  • Pharmacokinetics of artesunate in the domestic pig. Véronique Sinou; Nicolas Taudon; Joël Mosnier; Christian Aglioni; Françoise M. M. Bressolle; Daniel Parzy // Journal of Antimicrobial Chemotherapy (JAC);Sep2008, Vol. 62 Issue 3, p566 

    : Objectives The aim was to study the pharmacokinetic profile of artesunate and its metabolite dihydroartemisinin (DHA) in a pig model. : Methods Thirteen pigs received either intravenous (iv) or intramuscular (im) artesunate (60 mg), with the alternative preparation given 24 h later in an open...

  • Population Pharmacokinetics of Mycophenolic Acid. de Winter, Brenda C. M.; van Gelder, Teun; Glander, Petra; Cattaneo, Dario; Tedesco-Selva, Helio; Neumann, Irmgard; Hilhrands, Luuk; van Hest, Reinier M.; Pescovitz, Mark D.; Budde, Klemens; Mathot, Ron A. A. // Clinical Pharmacokinetics;2008, Vol. 47 Issue 12, p827 

    Objective: The pharmacokinetics of mycophenolic acid (MPA) were compared in renal transplant patients receiving either mycophenolate mofetil (MMF) or enteric-coated mycophenolate sodium (EC-MPS). Methods: MPA concentration-time profiles were included from EC-MPS- (n = 208) and MMF-treated (n =...

  • Propofol pharmacokinetics in China: A multicentric study. Hong-bo Ye; Jin-heng Li; Jian-zhong Rui; Hong Zheng; Xin-an Zhang; Xin-jin Chi; Wen-ying Chen; Jian-guo Xu // Indian Journal of Pharmacology;Jun2012, Vol. 44 Issue 3, p393 

    Objective: A multicenter population pharmacokinetics study of propofol was performed to establish a new population model. Materials and Methods: Three thousand two hundred and fifty-nine blood samples of 220 participants were measured by HPLC-UV or HPLC-FLU or GC-MS. Target-controlled infusion...

  • III-Standard deviation. Swinscow, T.D.V. // British Medical Journal;6/5/1976, Vol. 1 Issue 6022, p1393 

    Focuses on the use of standard deviation calculation in the measurement of urinary concentration of lead. Theoretical basis of standard deviation; Determination of Gaussian distribution patterns; Usefulness of standard deviation as basis for interpreting data in terms of probability.

  • Esterase Activities in the Blood, Liver and Intestine of Several Preclinical Species and Humans. Berry, Loren M.; Wollenberg, Lance; Zhiyang Zhao // Drug Metabolism Letters;Apr2009, Vol. 3 Issue 2, p70 

    Species and tissue differences in the activity of three major classes of esterases, carboxylesterase (CE), butyryl-cholinesterase (BChE) and paraoxonase (PON), were studied. Substantial species differences in activity of these esterases were observed between the mouse, rat, dog monkey and human....

  • Determination of Nicotine and Cotinine in Urine by Headspace Solid Phase Microextraction Gas Chromatography with Mass Spectrometric Detection. Karačonji, Irena Brčić; Skender, Ljiljana; Karačić, Višnja // Acta Chimica Slovenica;2007, Vol. 54 Issue 1, p74 

    A simple and rapid method for determination of low concentrations of nicotine and cotinine in urine has been developed. The method is based on headspace solid phase microextraction (HS-SPME) followed by gas chromatographic determination and mass spectrometric detection (GC-MS). The calibration...

  • Age-Related Brain Cholinesterase Inhibition Kinetics following In Vitro Incubation with Chlorpyrifos-Oxon and Diazinon-Oxon. Ahmed A. Kousba; Torka S. Poet; Charles Timchalk // Toxicological Sciences;Jan2007, Vol. 95 Issue 1, p147 

    Chlorpyrifos and diazinon are two commonly used organophosphorus insecticides (OPs), and their primary mechanism of action involves the inhibition of acetylcholinesterase by their metabolites chlorpyrifos-oxon (CPO) and diazinon-oxon (DZO), respectively. The study objectives were to assess the...

  • Determination of Ozonization Reaction Rate Constants of Aromatic Pollutants and QSAR Study. Jiang, Jian; Yue, Xiang; Chen, Qiu; Gao, Zhen // Bulletin of Environmental Contamination & Toxicology;Dec2010, Vol. 85 Issue 6, p568 

    Rate constants of ozone with 39 aromatic compounds in aqueous solution were determined at 298 K. And optimized calculation was carried out at B3LYP/6-311G** level with DFT method. 10 molecular parameters obtained from calculations were selected as the descriptors to establish QSAR models for...

  • Sensitive Kinetic Spectrophotometric method for Trace determination of Cerium (IV) Based on decolarization of Nile blue by potassium Periodate. Tavallali, H.; Asrari, E.; Siahoe, M. A. Ameri // International Journal of ChemTech Research;Apr2009, Vol. 1 Issue 2, p359 

    A simple and sensitive kinetic spectrophotometric method for the determination of trace amounts of cerium (IV), based on the catalytic effect of Ce (IV) on the oxidation of Nile blue (NB) by potassium periodate is described. The reaction rate was monitored spectrophotometrically by measuring the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics