TITLE

UPLC-MS/MS determination of doxazosine in human plasma

AUTHOR(S)
Al-Dirbashi, Osama Y.; Aboul-Enein, Hassan Y.; Jacob, Minnie; Al-Qahtani, Khalid; Rashed, Mohamed S.
PUB. DATE
September 2006
SOURCE
Analytical & Bioanalytical Chemistry;Sep2006, Vol. 385 Issue 8, p1439
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A sensitive, selective and rapid method for the analysis of doxazosine (DOX) in human plasma based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) is described. DOX and tamsulosin, used as an internal standard (IS), were extracted by liquid-liquid extraction, and the chromatography was performed on a C18 UPLC column packed with 1.7 μm particles. The total run time was 2 min. Detection was achieved by the multiple reaction monitoring of the following transitions: m/z 452→344 and m/z 409→228 for DOX and IS, respectively. Transitions of m/z 452→247 and m/z 409→271 were also collected for confirmation purposes. The calibration curve based on peak area ratio was linear up to at least 100 ng ml−1, with a detection limit of 0.02 ng ml−1 (a signal-to-noise ratio of 3). The method showed satisfactory reproducibility, and the short-term stability of the analyte was assessed. The method was successfully applied to the analysis of DOX in human plasma.
ACCESSION #
21857584

 

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