Rapid analysis of native neomycin components on a portable capillary electrophoresis system with potential gradient detection

LingLing Yuan; HongPing Wei; HuaTao Feng; Li, Sam F. Y.
September 2006
Analytical & Bioanalytical Chemistry;Sep2006, Vol. 385 Issue 8, p1575
Academic Journal
A simple method based on capillary electrophoresis with potential gradient detection was developed to separate and detect neomycin components within 4 min without a derivatization step. Satisfactory separation and good repeatability were obtained using a separation buffer composed of 1 m M ammonium citrate (pH 3.5). The linearity of the method ranged from 10 to 1000 ppm with a limit of detection for neomycin B of about 7 ppm. After a simple dilution and filtering pretreatment step, neomycin components in three real samples were successfully analyzed without any major interference. Due to its simplicity and reliability, this method could provide an excellent alternative to the assays currently listed in U.S. and European Pharmacopoeia. The experiments were performed on a portable capillary electrophoresis system and, hence, the method can be readily applied to field analysis and point-of-care testing. [Figure not available: see fulltext.]


Related Articles

  • HPCE 2004: 17th Int'l Symposium on Microscale Separations and Capillary Electrophoresis.  // LC-GC Europe;Dec2003, Vol. 16 Issue 12, p824 

    Provides information on the 17th International Symposium on Microscale Separations and Capillary Electrophoresis in Salzburg, Austria. Topics that will be discussed during the symposium; List of events and activities; Contact information.

  • Evaluation of methylation degree from formalin-fixed paraffin-embedded DNA extract by field-amplified sample injection capillary electrophoresis with UV detection. Zinellu, Angelo; Sotgia, Salvatore; De Murtas, Valentina; Cossu-Rocca, Paolo; De Miglio, Maria Rosaria; Muroni, Maria Rosaria; Mura, Antonica; Uras, Maria Gabriela; Contini, Marcella; Deiana, Luca; Carru, Ciriaco // Analytical & Bioanalytical Chemistry;Feb2011, Vol. 399 Issue 3, p1181 

    lterations in global DNA methylation are implicated in several pathobiological processes. The tissues stored as paraffin blocks represent an important source of DNA for retrospective genetic and epigenetic analysis on a large scale. Therefore, we developed the first capillary electrophoresis...

  • Sensitive and efficient determination of gabapentin in human serum based on capillary electrophoresis with laser-induced fluorescence detection. Cao, Li-Wei; Hu, Yang; Liang, Si-Liu; Li, Cong; Meng, Jian-Xin // Journal of Analytical Chemistry;Jul2013, Vol. 68 Issue 7, p639 

    A sensitive and efficient analytical method for gabapentin ( GBP) in human serum based on capillary electrophoresis ( CE) with laser-induced fluorescence ( LIF) detection has been established. 6-Oxy-( N-succinimidyl acetate)-9-(2′-methoxycarbonyl) fluorescein ( SAMF), a new synthesized...

  • Coupling of capillary electroseparation techniques with mass spectrometric detection. Klampfl, Christian W.; Buchberger, Wolfgang // Analytical & Bioanalytical Chemistry;Jun2007, Vol. 388 Issue 3, p533 

    The article offers a look at the trends in coupling of capillary electroseparation techniques. Capillary electroseparation involves techniques in which separation mechanisms are based on electrophoretic principles. The electroseparation techniques coupled with mass spectrometric detection...

  • CE determination of small ions: methods and techniques. Padarauskas, Audrius // Analytical & Bioanalytical Chemistry;Dec2005, Vol. 384 Issue 1, p132 

    This paper provides an overview on the current status of capillary electrophoresis (CE) in the analysis of inorganic and charged small organic species. The various CE strategies used to improve the separation of ionic analytes are summarized. Technical developments in the design of improved...

  • Recent advances in the application of capillary electrophoresis to neuroscience. Powell, Paula R.; Ewing, Andrew G. // Analytical & Bioanalytical Chemistry;Jun2005, Vol. 382 Issue 3, p581 

    With fast separation times (seconds to minutes), minimal sample requirements (nanoliters to femtoliters), and excellent mass detection limits (femtomole to zeptomole), capillary electrophoresis (CE) is ideally suited for in vitro and in vivo sampling of neurological samples with a high degree of...

  • Dispersion due to wall interactions in microfluidic separation systems. Datta, Subhra; Ghosal, Sandip // Physics of Fluids;Jan2008, Vol. 20 Issue 1, p012103 

    The transport of a solute in a straight microchannel of axially variable cross-sectional shape in the presence of an inhomogeneous flow field and an adsorption-desorption process on the wall is studied, motivated by applications to capillary electrophoresis and open-channel capillary...

  • Sensitivity improvement in capillary electrophoresis using organo-aqueous separation buffers and thermal lens detection. Bendrysheva, Svetlana N.; Proskurnin, Mikhail A.; Pyell, Ute; Faubel, Werner // Analytical & Bioanalytical Chemistry;Sep2006, Vol. 385 Issue 8, p1492 

    It is shown that organo-aqueous separation buffers show much promise when used in capillary electrophoresis separations with photothermal (thermal lens) detection systems. Acetonitrile–water and methanol–water mixtures were selected, as conventionally used in capillary...

  • Dynamic monitoring of glucagon secretion from living cells on a microfluidic chip. Shackman, Jonathan; Reid, Kendra; Dugan, Colleen; Kennedy, Robert // Analytical & Bioanalytical Chemistry;Jun2012, Vol. 402 Issue 9, p2797 

    A rapid microfluidic based capillary electrophoresis immunoassay (CEIA) was developed for on-line monitoring of glucagon secretion from pancreatic islets of Langerhans. In the device, a cell chamber containing living islets was perfused with buffers containing either high or low glucose...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics