TITLE

Upregulation of COX-1 and COX-2 in nasal polyps in cystic fibrosis

AUTHOR(S)
Roca-Ferrer, J.; Pujols, I.; Gartner, S.; Moreno, A.; Pumarola, F.; Mullol, J.; Cobos, N.; Picado, C.
PUB. DATE
July 2006
SOURCE
Thorax;Jul2006, Vol. 61 Issue 7, p592
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Since abnormalities in prostanoid metabolism occur in the lower airway of patients with cystic fibrosis (CF), it is likely that they could also be detected in the nose. Methods: The degree of mRNA and protein expression of cyclo-oxygenase (COX) enzymes 1 (COX-1) and 2 (COX-2) was examined using quantitative reverse competitive polymerase chain reaction (RT-PCR) and Western blot analysis in the nasal polyps from 10 patients with CF, nasal polyps from 10 non-CF patients and 11 nasal mucosa specimens. The results are presented as 106 cDNA molecules/µg total RNA and the densitometric ratio between protein and β-actin. Results: COX-1 mRNA levels were significantly higher in CF nasal polyps (median 2.34, 25-75th percentiles 1.6-3.2) than in the nasal mucosa (0.78, 0.11-1.21), while there was no difference with non-CF nasal polyps (1.11, 0.80-3.15). COX-1 protein levels were significantly higher in CF nasal polyps (3.63, 2.71-4.27) than in nasal mucosa (1.55, 0.66-2.33) and non-CF nasal polyps (2.19, 1.72-3.68). COX-2 mRNA was significantly higher in CF nasal polyps (3.34, 2.42-7.05) than in nasal mucosa (1.69, 0.19-3.50). No differences were found in COX-2 mRNA expression between CF and non-CF polyps (1.38, 0.12-6.07). COX-2 protein levels were also significantly higher in CF nasal polyps (0.23, 0.04-0.34) than in non-CF nasal polyps (0.011, 0.009-0.016) or nasal mucosa (0.014, 0.014-0.016). Conclusions: Upregulation in the expression of COX-1 and COX-2 could explain the high production of prostanoids reported in CF. These findings raise questions regarding the potential use of selective or non-selective COX-2 non-steroidal anti-inflammatory treatment in CF.
ACCESSION #
21715225

 

Related Articles

  • Endoscopic view of cystic fibrosis with nasal polyposis. Christmas Jr., Dewey A.; Mirante, Joseph P.; Yanagisawa, Eiji // ENT: Ear, Nose & Throat Journal;May2007, Vol. 86 Issue 5, p262 

    The article provides an endoscopic view of cystic fibrosis (CF) with nasal polyposis in a 25-year-old man. It says that computed tomography (CT) of the sinuses revealed opacification of the right maxillary and ethmoid sinuses. Also noted is that most patients with CF manifest chronic obstructive...

  • Analysis of most common CFTR mutations in patients affected by nasal polyps. Kostuch, Marzena; Klatka, Janusz; Semczuk, Andrzej; Wojcierowski, Jacek; Kulczycki, Lucas; Oleszczuk, Jan // European Archives of Oto-Rhino-Laryngology;Dec2005, Vol. 262 Issue 12, p982 

    Nasal polyps, a chronic inflammatory disease occurring in the nose and para-nasal sinuses, result from several different causes, including cystic fibrosis (CF). Forty-four patients affected by nasal polyps were admitted to the Department of Otolaryngology, Lublin University School of Medicine,...

  • Night Blindness and Diabetes in a Non-Caucasian Cystic Fibrosis Patient in Iran. Tabaripour, Reza; Esmaeili Dooki, Mohammad Reza; Akhavan-Niaki, Haleh // World Applied Sciences Journal;2013, Vol. 23 Issue 6, p837 

    Cystic fibrosis (CF) is an autosomal recessive lethal disease that is predominantly occur in the caucasian populations and causes many clinical signs. The major clinical signs are pancreatic insufficiency and progressive lung disease but night blindness and diabetes are less well known. We...

  • Cystic fibrosis lung disease following infection with Pseudomonas aeruginosa in Cftr knockout mice using novel non-invasive direct pulmonary infection technique . C Guilbault; P Martin; D Houle; M-L Boghdady; M-C Guiot; D Marion; D Radzioch // Laboratory Animals;Jun2005, Vol. 39 Issue 3, p336 

    To better understand the mechanism of lung infection with Pseudomonas aeruginosa (P. aeruginosa), many techniques have been developed in order to establish lung infection in rodents. A model of chronic lung infection, using tracheotomy to inoculate the bacteria, has been extensively used in the...

  • Lack of correlation between pulmonary disease and cystic fibrosis transmembrane conductance regulator dysfunction in cystic fibrosis: a case report. Levy, Hara; Cannon, Carolynn L.; Asher, Daniel; García, Christopher; Cleveland, Robert H.; Pier, Gerald B.; Knowles, Michael R.; Colin, Andrew A.; García, Christopher // Journal of Medical Case Reports;2010, Vol. 4 Issue 1, p1 

    Introduction: Mutations in both alleles of the cystic fibrosis transmembrane conductance regulator gene result in the disease cystic fibrosis, which usually manifests as chronic sinopulmonary disease, pancreatic insufficiency, elevated sodium chloride loss in sweat, infertility...

  • Evidence based guidelines for the performance of the sweat test for the investigation of cystic fibrosis in the UK. Baumer J H // Archives of Disease in Childhood;Dec2003, Vol. 88 Issue 12, p1126 

    A well produced evidence based guideline has been developed in response to a national audit that demonstrated wide variations in the performance of sweat tests. Accurate and reliable sweat test results will be particularly important with the advent of neonatal screening. The guideline...

  • Andy versus Cystic Fibrosis. Lipman, Eva // Exceptional Parent;Nov2003, Vol. 33 Issue 11, p30 

    Recounts a mother's struggle in raising up a child with cystic fibrosis (CF). Clinical manifestations and epidemiology of the disease; Symptoms observed in Andy Lipman, the second born child of Charles and Eva Lipman with CF; Fears and excitement felt by the parents on the birth of Andy:...

  • Cystic fibrosis: ultrastructural changes of nasal mucosa. Knipping, Stephan; Holzhausen, Hans; Riederer, Andreas; Bloching, Marc // European Archives of Oto-Rhino-Laryngology;Dec2007, Vol. 264 Issue 12, p1413 

    Cystic fibrosis (CF) is an inherited multisystemic disorder that results in a generalized dysfunction of exocrine glands. Besides chronic obstructive pulmonary diseases, chronic sinusitis, nasal polyposis and hypertrophy of the inferior turbinates with nasal airway obstruction are typical signs....

  • Bacterial physiology: A metabolic milieu. Molloy, Sheilagh // Nature Reviews Microbiology;May2014, Vol. 12 Issue 5, p315 

    The article reports on a study that examines the core metabolic functions in the cystic fibrosis (CF) lung microbiome. It provides details of the study that extracts microbial DNA and RNA from sputum samples in six different patients with CF. It also outlines the result of the study which...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics