TITLE

Parallel factor analysis combined with PLS regression applied to the on-line monitoring of Pichia pastoris cultures

AUTHOR(S)
Surribas, Anna; Amigo, José Manuel; Coello, Jordi; Montesinos, José Luis; Valero, Francisco; Maspoch, Santiago
PUB. DATE
September 2006
SOURCE
Analytical & Bioanalytical Chemistry;Sep2006, Vol. 385 Issue 7, p1281
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Various key variables (biomass, substrate and product) of bioprocesses should be monitored in order to retrieve useful information on the system, with the biomass (the cell density) the principal target. Although several analytical methods have been adapted and used to monitor the evolution of cell density evolution in cultures, a general method for performing this determination has not yet been established, as each technique has its own advantages and drawbacks. In the present work, noninduced glycerol batch cultures (for which biomass and substrate are the key variables) were monitored using multiwavelength fluorescence spectroscopy. The data gathered were modelled via PARAFAC-PLS chemometric methodologies, resulting in important qualitative and quantitative information about the behaviours of different biogenic fluorophors in batch cultures of the yeast Pichia pastoris. This information was used to predict the target process variables in such cultures; this permitted the applicability of this combined technique to bioprocess monitoring to be assessed.
ACCESSION #
21625740

 

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