TITLE

Purification and Structural Characterization of Human ERp29

AUTHOR(S)
Jinbiao Zheng; Xingang Liu; Xiaomin Yan; Linsen Dai; Chaoneng Ji
PUB. DATE
August 2006
SOURCE
Protein & Peptide Letters;Aug2006, Vol. 13 Issue 8, p753
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
ERp29 is a major resident of the endoplasmic reticulum (ER) and is postulated to play an important molecular chaperone role in most animal cells. Human ERp29 was isolated to homogeneity in high yield by using a bacterial expression system. Its secondary structure was studied by circular dichroism (CD), Fourier transformed infrared spectroscopy (FTIR) and Raman spectroscopy and it was found that human ERp29 comprises significant _-helical structure. The details of its temperature-induced conformational changes was studied by CD and FTIR for the first time, revealing that the protein is stable below 50 �C and has two distinct structural transitions between 50 �C and 70 �C. This may shed light on ERp29's inability to protect substrate proteins against thermal aggregation.
ACCESSION #
21543552

 

Related Articles

  • Structure Study of Recombinant RGD-Hirudin by Vibrational and Circular Dichroism Spectroscopy. Xingang Liu; Wei Mo; Linsen Dai; Xiaomin Yan; Houyan Song // Protein & Peptide Letters;Jan2006, Vol. 13 Issue 1, p47 

    The secondary structure of a new type of recombinant RGD-hirudin, which has the activities of anti-thrombin and anti-platelet aggregation, has been studied by Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy and circular dichroism (CD) methods. The distribution of various...

  • Quality control of transmembrane domain assembly in the tetraspanin CD82. Cannon, Kurt S.; Cresswell, Peter // EMBO Journal;5/15/2001, Vol. 20 Issue 10, p2443 

    Retention of misfolded proteins in the endoplasmic reticulum (ER) is a primary mechanism of quality control. To discover whether quality control can monitor assembly inside the hydrophobic ER membrane, we characterized the folding and transport of the tetraspanin glycoprotein CD82. Truncated...

  • Thermal aggregation and ion-induced cold-gelation of bovine serum albumin. Navarra, Giovanna; Giacomazza, Daniela; Leone, Maurizio; Librizzi, Fabio; Militello, Valeria; San Biagio, Pier Luigi // European Biophysics Journal;Apr2009, Vol. 38 Issue 4, p437 

    Protein cold-gelation has recently received particular attention for its relevance in bio and food technology. In this work, we report a study on bovine serum albumin cold-gelation induced by copper or zinc ions. Metal-induced cold-gelation of proteins requires two steps: during the first one,...

  • Free Cysteine Modulates the Conformation of Human C/EBP Homologous Protein. Singh, Vinay K.; Rahman, Mona N.; Munro, Kim; Uversky, Vladimir N.; Smith, Steven P.; Jia, Zongchao // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    The C/EBP Homologous Protein (CHOP) is a nuclear protein that is integral to the unfolded protein response culminating from endoplasmic reticulum stress. Previously, CHOP was shown to comprise extensive disordered regions and to selfassociate in solution. In the current study, the intrinsically...

  • Free Cysteine Modulates the Conformation of Human C/EBP Homologous Protein. Singh, Vinay K.; Rahman, Mona N.; Munro, Kim; Uversky, Vladimir N.; Smith, Steven P.; Jia, Zongchao // PLoS ONE;Apr2012, Vol. 7 Issue 4, p1 

    The C/EBP Homologous Protein (CHOP) is a nuclear protein that is integral to the unfolded protein response culminating from endoplasmic reticulum stress. Previously, CHOP was shown to comprise extensive disordered regions and to selfassociate in solution. In the current study, the intrinsically...

  • ATR and transmission analysis of pigments by means of far infrared spectroscopy. Kendix, Elsebeth L.; Prati, Silvia; Joseph, Edith; Sciutto, Giorgia; Mazzeo, Rocco // Analytical & Bioanalytical Chemistry;Jun2009, Vol. 394 Issue 4, p1023 

    In the field of FTIR spectroscopy, the far infrared (FIR) spectral region has been so far less investigated than the mid-infrared (MIR), even though it presents great advantages in the characterization of those inorganic compounds, which are inactive in the MIR, such as some art pigments,...

  • Fourier-Transform Raman Spectroscopy.  // Physics Today;May88, Vol. 41 Issue 5, p108 

    Introduces Spex Industries' automated Raman spectrometry system with Fourier-transform capabilities. Use of the Fourier-transform Raman analysis in the near infrared with interferometric techniques; Incorporation of a continuous wave Nd:YAG laser for sample excitation.

  • Coherent Raman and infrared spectroscopy of HCN complexes in free jet expansions and in equilibrium samples. Maroncelli, M.; Hopkins, G. A.; Nibler, J. W.; Dyke, T. R. // Journal of Chemical Physics;9/1/1985, Vol. 83 Issue 5, p2129 

    Small hydrogen bonded complexes of HCN and DCN have been examined in the gas phase using FTIR and photoacoustic Raman spectroscopy (PARS). Four distinct bands are seen and assigned to CN stretching modes of the HCN dimer (D) and trimer (T). Previous microwave studies have shown the HCN dimer to...

  • Infrared and Raman features of a series of α,ω-bis(arylthio)oligothiophenes as molecular wires. A Ï€-electron delocalization efficiency study. Casado, J.; Hicks, R. G.; Hernández, V.; Myles, D. J. T.; Ruiz Delgado, M. C.; López Navarrete, J. T. // Journal of Chemical Physics;1/22/2003, Vol. 118 Issue 4, p1912 

    Fourier transform infrared and Raman spectra of a novel series of neutral α, ω-bis(mesitylthio)oligothiophenes and some α,ω-bis(mesitylthio)oligothiophenes containing ethylenedioxy groups at the β positions, with various chain lengths have been investigated. Because these...

Share

Read the Article

Other Topics