TITLE

Mutations in Helicobacter pylori porD and oorD Genes May Contribute to Furazolidone Resistance

AUTHOR(S)
Zhaoliang Su; Huaxi Xu; Chiyu Zhang; Shihe Shao; Liangju Li; Hua Wang; Huifang Wang; Gufeng Qiu
PUB. DATE
June 2006
SOURCE
Croatian Medical Journal;2006, Vol. 47 Issue 3, p410
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Aim To determine the rate of furazolidone resistance of Helicobacter pylori (H. pylori) isolated from gastric biopsy specimens and to explore the relationship between genetic mutations in porD and oorD genes of H. pylori and its resistance to the antibiotic. Methods Gastric biopsy was performed in 83 adult patients aged 31-77 years with gastric complaints. H. pylori was isolated from biopsy specimens of 46 patients. E-test and 2-fold agar dilution method were used to determine the rate of H. pylori resistance to furazolidone. The genes porD and oorD from susceptible and resistant isolates were amplified by polymerase chain reaction (PCR), and their PCR products were sequenced. Results Resistance to furazolidone was found in 8.7% of H. pylori isolates and 6 mutations were detected in porD and oorD genes of the resistant isolates. Three mutations - G353A, A356G, and C357T - occurred in porD and the other mutations - A041G, A122G, C349A(G) - occurred in oorD genes. Conclusions Changes in 6 amino acids may be associated with the resistance of H. pylori to furazolidone.
ACCESSION #
21416989

 

Related Articles

  • Furazolidone, co-amoxiclav, colloidal bismuth subcitrate, and esomeprazole for patients who failed to eradicate Helicobacter pylori with triple therapy. Abbas, Zaigham; Yakoob, Javed; Abid, Shahab; Jafri, Wasim; Islam, Muhammad; Azam, Zahid; Hilal, Imran // Digestive Diseases & Sciences;Sep2009, Vol. 54 Issue 9, p1953 

    There is increasing evidence of Helicobacter pylori (H. pylori) resistance to the classical triple therapy consisting of a proton-pump inhibitor and clarithromycin with either amoxicillin or metronidazole. This study is aimed at establishing the efficacy and safety of a 14-day regimen to...

  • Treatment for Helicobacter pylori infection, an overview. Hajiani, Eskandar // Jundishapur Journal of Microbiology;2009, Vol. 2 Issue 2, p41 

    The best treatment schedule for eradication of Helicobacter pylori in Iran is a type of quadruple therapy for a minimum duration of two weeks. Since, clarithromycin and furazolidone based quadruple regimen has the highest eradication rate, it can be considered as the first line therapy....

  • Helicobacter pylori treatment in the era of increasing antibiotic resistance. Graham, David Y.; Fischbach, Lori // Gut;Aug2010, Vol. 59 Issue 8, p1143 

    With few exceptions, the most commonly recommended triple Helicobacter pylori regimen (proton pump inhibitor (PPI), amoxicillin and clarithromycin) now provides unacceptably low treatment success. A review of worldwide results suggests that successful eradication using a triple regimen is not...

  • Distribution of Inflammation and Atrophy in the Stomach of Helicobacter pylori-Positive and -Negative Patients with Chronic Gastritis. Satoh, Kiichi; Kimura, Ken; Taniguchi, Yushi; Yoshida, Yukio; Kihira, Ken; Takimoto, Takuya; Kawata, Hiroshi; Saifuku, Koji; Ido, Kenichi; Takemoto, Tadayoshi; Ota, Yukiko; Tada, Masahiro; Karita, Mikio; Sakaki, Nobuhiro; Hoshihara, Yoshio // American Journal of Gastroenterology;May1996, Vol. 91 Issue 5, p963 

    Objectives: To investigate the extent of inflammation and atrophy in the stomach of Helicobacter pylori-positive and -negative patients with chronic gastritis. Methods: Endoscopy with biopsies from the lesser curvatures of the antrum, angulus, middle body, and the greater curvature of the middle...

  • Storage Temperature of the Unbuffered Rapid Urease Test. Fook-Hong Ng; Siu-Yin Wong; Wing-Fung Ng // American Journal of Gastroenterology;Dec1997, Vol. 92 Issue 12, p2230 

    Objectives: The unbuffered rapid urease test (RUT) is an accurate, rapid, and inexpensive method for detecting Helicobacter pylori. However, it is generally recommended that the reagent be prepared daily. This prospective study was undertaken to evaluate the shelf life of our unbuffered...

  • Endoscopy in Dyspeptic Patients: Is Gastric Mucosal Biopsy Useful? Vaira, Dino; Holton, John; Osborn, John; D'Anna, Luigi; Romanos, Andreas; Falzon, Mary; McNeil, Ian // American Journal of Gastroenterology;Jun1990, Vol. 85 Issue 6, p701 

    Should dyspeptic patients coming to endoscopy with "normal" findings also he biopsied? To assess this, we studied the sensitivity and specificity of endoscopic examination compared with histology, microscopic evidence of Helicobacter pylori, CP-TEST, culture, and serum IgG and IgA antibody...

  • The Pyloritek Test and the CLO Test: Accuracy and Incremental Cost Analysis. Puetz, Tom; Vakil, Nimish; Phadnis, Suhas; Dunn, Bruce; Robinson, James // American Journal of Gastroenterology;Feb1997, Vol. 92 Issue 2, p254 

    Objective: The aim of this study was to compare the Pyloritek test (a 1-h rapid urease test) to the widely used CLO test. Methods: Seventy-one patients undergoing upper endoscopy were studied. All patients gave informed consent. A single antral biopsy specimen was obtained for the CLO test, and...

  • FORMULATION AND IN VITRO EVALUATION OF FURAZOLIDONE MUCOADHESIVE MICROSPHERES. Venkateswaramurthy, N.; Sambathkumar, R.; Vijayabaskaran, M.; Perumal, P. // International Journal of Pharmacy & Pharmaceutical Sciences;Sep2010, Vol. 2 Issue 3, p104 

    The purpose of this research was to formulate and systematically evaluate in vitro performances of mucoadhesive Furazolidone microspheres for the potential use of treating gastric and duodenal ulcers, which were associated with Helicobacter pylori. Furazolidone mucoadhesive microspheres were...

  • Saliva Secretion and Efficacy of Helicobacter Pylori Eradication in Peptic Ulcer Patients. Vahedi, M.; Khalilian, AR.; Abdollahzadeh, Sh.; Shafiei, N. // Journal of Dentistry of Tehran University of Medical Sciences;2011, Vol. 8 Issue 4, p165 

    Objective: It has been noted that the presence of Helicobacter pylori (H. pylori) in the oral cavity may affect the outcome of eradication therapy. This condition is associated with the recurrence of gastric infection. The optimum secretion of saliva promotes oral health consequently influencing...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics