TITLE

CXCR3 and αE137 integrin identify a subset of CD8+ mature thymocytes that share phenotypic and functional properties with CD8+ gut intraepithelial lymphocytes

AUTHOR(S)
Annunziato, F.; Cosmi, L.; Liotta, F.; Lazzeri, E.; Romagnani, P.; Angeli, R.; Lasagni, L.; Manetti, R.; Marra, F; Gerard, C.; Petrai, I.; Dello Sbarba, P.; Tonelli, F.; Maggi, E.; Romagnani, S.
PUB. DATE
July 2006
SOURCE
Gut;Jul2006, Vol. 55 Issue 7, p961
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: We previously demonstrated the existence of two distinct subsets of T cell receptor (TCR)αβ+CD8cαβ+ single positive (SP) cells in human postnatal thymus which express the chemokine receptor CCR7 or CXCR3 and migrate in vitro in response to their specific ligands. Aim: To investigate whether these two CD8+ thymocyte subsets had distinct peripheral colonisation. Methods: TCRαβ+CD8+ SP cells were obtained from normal postnatal thymus, mesenteric lymph node (LNs), small bowel, and peripheral blood (PB) specimens. Cells were then evaluated for expression of surface molecules, cytolytic potential, telomere length, and profile of cytokine production. Results: CD8+CCR7+CXCR3- thymocytes exhibited CD62L, in common with those which localise to LNs. In contrast, CD8+CCR7-CXCR3+ thymocytes lacked CD62L but exhibited CD103, similar to intraepithelial lymphocytes (IELs) present in the gut mucosa where the CXCR3 ligand, CXCL10, and the CD103 ligand, E-cadherin, are highly and consistently expressed. In addition, thymocytes and gut CD8+CXCR3+CD103+ cells showed comparable telomere length, which was higher than that of PB CXCR3+CD8+ T cells. However, both of these populations contained perforin and granzyme A, and displayed the ability to produce interferon γ and interleukin 2. Of note, CXCR3 deficient, in comparison with wild-type C57Black/6, mice showed decreased proportions of CD3+CD8αβ+ and increased proportions of CD3+CD8αα+ lymphocytes at gut level. Moreover, adoptive transfer of CD3+CD8αβ+ thymocytes from wild-type into CXCR3 deficient mice resulted in a significant increase in CD3+CD8αβ+ T cells in the gut mucosa but not in other tissues. Conclusions: The results of this study demonstrate the existence of a previously unrecognised subset of TCRαβ+CD8αβ+ SP CXCR3+CD103+ thymocytes which share phenotypic and functional features with CD8+ IELs, thus suggesting the possibility of their direct colonisation of the gut mucosa.
ACCESSION #
21399619

 

Related Articles

  • CTLA-4 suppresses the pathogenicity of self antigen—specific T cells by cell-intrinsic and cell-extrinsic mechanisms. Ise, Wataru; Kohyama, Masako; Nutsch, Katherine M.; Hyang Mi Lee; Suri, Anish; Unanue, Emil R.; Murphy, Theresa L.; Murphy, Kenneth M. // Nature Immunology;Feb2010, Vol. 11 Issue 2, p129 

    The inhibitory immunoregulatory receptor CTLA-4 is critical in maintaining self-tolerance, but the mechanisms of its actions have remained controversial. Here we examined the antigen specificity of tissue-infiltrating CD4+ T cells in Ctla4−/− mice. After adoptive transfer, T cells...

  • Rare expression of T-cell markers in classical Hodgkin's lymphoma. Tzankov, Alexandar; Bourgau, Caroline; Kaiser, Alexandra; Zimpfer, Annette; Maurer, Robert; Pileri, Stefano A.; Went, Philip; Dirnhofer, Stephan // Modern Pathology;Dec2005, Vol. 18 Issue 12, p1542 

    Hodgkin's and Reed–Sternberg cells of classical Hodgkin's lymphoma are primarily of B-cell origin, although there are instances of T-cell antigen expression suggesting T-cell origin. We comprehensively analyzed expression of various T-cell antigens in 259 classical Hodgkin's lymphoma...

  • Thymocyte development: The identification of THEMIS. Leavy, Olive // Nature Reviews Immunology;Sep2009, Vol. 9 Issue 9, p604 

    The article focuses on three studies which examine the role of thymocyte-expressed molecule involved in selection (THEMIS) downstream of T cell receptor in thymocytes. Research groups made use of gene-targeted or ethylnitrosourea (ENU)-induced THEMIS-deficient mice with no obvious abnormalities....

  • Diagnostic Value of T-Cell Receptor Beta Gene Rearrangement Analysis on Peripheral Blood Lymphocytes of Patients with Erythroderma. Bakels, Victor; Van Oostveen, Johan W.; Gordijn, Roel L. J.; Walboomers, Jan M. M.; Meijer, Chris J. L. M.; Willemze, Rein // Journal of Investigative Dermatology;Nov91, Vol. 97 Issue 5, p782 

    Differentiation between Sézary's syndrome (SS) and benign forms of erythroderma may be extremely difficult. In this study T-cell receptor beta (TCRβ) gene rearrangement analysis was performed on peripheral blood lymphocytes (PBL) from 32 patients with erythroderma, including 10 patients...

  • Membranes as messengers in T cell adhesion signaling. Dustin, Michael L.; Philips, Mark R.; Bivona, Trever G. // Nature Immunology;Apr2004, Vol. 5 Issue 4, p363 

    Talin and RapL are components of molecular pathways that regulate the avidity of the integrin lymphocyte function-associated antigen 1 (LFA-1) for its ligand, intercellular adhesion molecule 1. In this review, we discuss recent advances in our understanding of LFA-1 affinity regulation and...

  • ROLUL LIMFOCITOZEI MONOCLONALE B ÃŽN ETIOLOGIA LEUCEMIEI LIMFATICE CRONICE. Cocişiu, Gabriela Ariadna; Deac, M. // Acta Medica Transilvanica;sep2011, Vol. 16 Issue 3, p104 

    Chronic lymphocytic leukemia (CLL) is among the most common types of leukemia. Recent studies suggest that a population of clonal B cells with the phenotype of CLL, can be detected in the general population, a condition now designated monoclonal B-cell lymphocytosis (MBL). Although all cases of...

  • Mucosal immunology: Oddball T cells. Bell, Elaine // Nature Reviews Immunology;May2003, Vol. 3 Issue 5, p358 

    Deals with the existence of T cells that are odd in nature. Information on T cells that express invariant receptors; Determination of cell types that mediate the selection of such cells.

  • In Vivo and In Vitro Effects of Antituberculosis Treatment on Mycobacterial Interferon-γ T Cell Response. Sauzullo, Ilaria; Mengoni, Fabio; Lichtner, Miriam; Massetti, Anna Paola; Rossi, Raffaella; Iannetta, Marco; Marocco, Raffaella; Del Borgo, Cosmo; Soscia, Fabrizio; Vullo, Vincenzo; Mastroianni, Claudio Maria // PLoS ONE;2009, Vol. 4 Issue 4, p1 

    Background: In recent years, the impact of antituberculous treatment on interferon (IFN)-c response to Mycobacterium tuberculosis antigens has been widely investigated, but the results have been controversial. The objective of the present study was: i) to evaluate longitudinal changes of IFN-c...

  • Geometrically Repatterned Immunological Synapses Uncover Formation Mechanisms. Figge, Marc Thilo; Meyer-Hermann, Michael // PLoS Computational Biology;Nov2006, Vol. 2 Issue 11, p1377 

    The interaction of T cells and antigen-presenting cells is central to adaptive immunity and involves the formation of immunological synapses in many cases. The surface molecules of the cells form a characteristic spatial pattern whose formation mechanisms and function are largely unknown. We...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics