TITLE

Study of a toxin–alkaline phosphatase conjugate for the development of an immunosensor for tetrodotoxin determination

AUTHOR(S)
Neagu, D.; Micheli, L.; Palleschi, G.
PUB. DATE
August 2006
SOURCE
Analytical & Bioanalytical Chemistry;Aug2006, Vol. 385 Issue 6, p1068
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This paper describes a direct competitive immunoenzymatic spectrophotometric assay (ELISA) for tetrodotoxin (TTX) determination and the adaptation of this method for use in an electrochemical assay format. The novelty of this work involves the use of the antigen labelled with alkaline phosphatase (AP); this conjugate was prepared in our laboratory as there is no commercially available conjugate of any kind for TTX. The new conjugate was characterized in terms of its affinity for the specific antibody as well as the residual concentration and the residual activity of the enzyme (AP) incorporated as label. The proposed method based on the new conjugate showed satisfactory results for TTX determination: for the spectrophotometric method the dynamic range was 4–15 ng mL−1 with a limit of detection (LOD) of 2 ng mL−1 ( R=0.9247), whereas for the electrochemical protocol the dynamic range was 2–50 ng mL−1 and the LOD was1 ng mL−1.
ACCESSION #
21396808

 

Related Articles

  • Frequent occurrence of the tetrodotoxin-bearing horseshoe crab Carcinoscorpius rotundicauda in Vietnam. Ha Viet Dao; Takata, Yoshinobu; Sato, Shigeru; Fukuyo, Yasuwo; Kodama, Masaaki // Fisheries Science;Apr2009, Vol. 75 Issue 2, p435 

    Tetrodotoxin (TTX) was detected by HPLC and LC-MS/MS in specimens of the horseshoe crab Carcinoscorpius rotundicauda collected from Tan Hai village, Vung Tau province, Vietnam, where cases of poisoning due to the consumption of horseshoe crab have frequently been reported. The soft tissue from...

  • Analytical Challenges: Determination of Tetrodotoxin in Human Urine and Plasma by LC-MS/MS. Kelvin Sze-Yin Leung; Bonnie Mei-Wah Fong; Yeuk-Ki Tsoi // Marine Drugs;Nov2011, Vol. 9 Issue 11, p2291 

    Tetrodotoxin (TTX) is a powerful sodium channel blocker found in puffer fish and some marine animals. Cases of TTX poisoning most often result from puffer fish ingestion. Diagnosis is mainly from patient's signs and symptoms or the detection of TTX in the leftover food. If leftover food is...

  • Evolutionary diversification of TTX-resistant sodium channels in a predator-prey interaction. Geffeney, Shana L.; Fujimoto, Esther; Brodie, Edmund D.; Ruben, Peter C. // Nature;4/7/2005, Vol. 434 Issue 7034, p759 

    Understanding the molecular genetic basis of adaptations provides incomparable insight into the genetic mechanisms by which evolutionary diversification takes place. Whether the evolution of common traits in different lineages proceeds by similar or unique mutations, and the degree to which...

  • Mutational Analysis of the Analgesic Peptide DrTx(1-42) Revealing a Functional Role of the Amino-Terminal Turn. Ping Li; Shunyi Zhu // PLoS ONE;Feb2012, Vol. 7 Issue 2, p1 

    Background: DrTx(1-42) (a carboxyl-terminally truncated version of drosotoxin) is a potent and selective blocker of tetrodotoxin-resistant (TTX-R) Na+ channels in rat dorsal root ganglion neurons with analgesic activity. This purpose is to identify key amino acids which are responsible for both...

  • Mutational Analysis of the Analgesic Peptide DrTx(1-42) Revealing a Functional Role of the Amino-Terminal Turn. Ping Li; Shunyi Zhu // PLoS ONE;Feb2012, Vol. 7 Issue 2, p1 

    Background: DrTx(1-42) (a carboxyl-terminally truncated version of drosotoxin) is a potent and selective blocker of tetrodotoxin-resistant (TTX-R) Na+ channels in rat dorsal root ganglion neurons with analgesic activity. This purpose is to identify key amino acids which are responsible for both...

  • Marine Toxins. Hungerford, James M.; Gago-Martinez, Ana // Journal of AOAC International;Mar/Apr2014, Vol. 97 Issue 2, p283 

    An introduction is presented in which the editor discusses various reports within the issue on topics including effect of marine toxins on human health, use of enzyme-linked immunosorbent assay (ELISA) for screening paralytic shellfish toxins (PSTs), and bioassays.

  • THE FREELANCE POISONER. Furlow, Bryant // New Scientist;01/20/2001, Vol. 169 Issue 2274, p30 

    Discusses the poison tetrodotoxin (TTX). Marine animals in which the neurotoxin has been found, including the pufferfish, the blue-ringed octopus, and the harlequin frog; How the poison shuts down the human nervous system; Discovery that bacteria produce the poison; Use of the toxin as a...

  • Effects of PKC and PKA Inhibitors on the cAMP-Stimulant-Induced Enhancement of Tetrodotoxin-Resistant Na+ (Nav1.8) Currents. Matsumoto, Shigeji; Yoshida, Shinki; Ikeda, Mizuho; Saiki, Chikako; Takeda, Mamoru // Open Pharmacology Journal;2008, Vol. 2, p17 

    The protein kinase C (PKC) inhibitor bisindolymaleimide Ro-31-8425 (Ro-31-8425) decreases the peak tetrodotoxin- resistant (TTX-R) Na+ (Nav1.8) current in nodose ganglion (NG) neurons, and this decrease is not altered by simultaneous application of 8-bromo-cAMP (8-Br-cAMP), phorbol 12-myristate...

  • An epitopic approach to designing and characterization of a multiple antigenic polypeptide against Botulinum neurotoxins A and E. Basiri, Mohsen; Mousavi, Seyed; Basiri, Hossein; Rasooli, Iraj // World Journal of Microbiology & Biotechnology;Sep2010, Vol. 26 Issue 9, p1659 

    Botulinum neurotoxin (BoNT) serotypes A, B and E are most frequently associated with human botulism. Recombinant vaccines against BoNTs are usually based on one or more domain(s) of the toxin molecule. In this study, we investigate a new-designed multiple antigenic polypeptide for serotypes A...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics