31P NMR study on the autophosphorylation of insulin receptors in the plasma membrane

Yili Ge; Hong Peng; Kaixun Huang
August 2006
Analytical & Bioanalytical Chemistry;Aug2006, Vol. 385 Issue 5, p834
Academic Journal
A nonradioactive 31P nuclear magnetic resonance (NMR) spectroscopy protocol has been developed and used to investigate in vitro autophosphorylation of insulin receptors. Optimum experimental conditions have been explored, and the effects of Mn2+ and phosphocreatine (PCr) on the determination of the phosphorylation reaction have been assayed. The method was used to monitor the time courses of the phosphorylation reaction in solution. The results from this NMR study were in agreement with observations of insulin receptor phosphorylation made by using Western blotting. [Figure not available: see fulltext.]


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