TITLE

Capillary HPLC—ICP MS mapping of selenocompounds in spots obtained from the 2-D gel electrophoresis of the water-soluble protein fraction of selenized yeast

AUTHOR(S)
Tastet, Laure; Schaumlöffel, Dirk; Bouyssiere, Brice; Lobinski, Ryszard
PUB. DATE
August 2006
SOURCE
Analytical & Bioanalytical Chemistry;Aug2006, Vol. 385 Issue 5, p948
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A method based on ICP collision-cell MS detection in capillary HPLC was developed to gain an insight into the purity and identity of selenium-containing proteins separated by 1-D and 2-D electrophoresis. The bands and spots obtained after the separation of water-soluble proteins in selenized yeast were digested with trypsin prior to chromatography. Selenium could be detected down to the subpicogram level. The method, assisted by information obtained by MALDI TOF MS on the 5000 Da cut-off fraction, permitted the purity of bands and spots to be estimated and the efficiency of tryptic digestion and the quantity of selenium present in individual peptides to be evaluated. Owing to the high sensitivity and the lack of matrix suppression effects, the method provided chromatograms with signal-to-noise ratios of 10–1000 in conditions where the common ES Q–TOF MS detection failed. [Figure not available: see fulltext.]
ACCESSION #
21288199

 

Related Articles

  • Expression of Hemoglobin Variant Migration by Capillary Electrophoresis Relative to Hemoglobin A2 Improves Precision. Keren, David F.; Shalhoub, Renee; Gulbranson, Ronald; Hedstrom, Deborah // American Journal of Clinical Pathology;Apr2012, Vol. 137 Issue 4, p660 

    We report the precision of the mean migration position of hemoglobin (Hb)S, HbC, HbG (Philadelphia), and HbD (Los Angeles) in 193 samples of whole blood assayed by capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC). By expressing the migration of Hb variants by CE...

  • Heroin-Induces Differential Protein Expression by Normal Human Astrocytes (NHA). Reynolds, Jessica L.; Mahajan, Supriya D.; Sykes, Donald; Nair, Madhavan P.N. // American Journal of Infectious Diseases;2006, Vol. 2 Issue 2, p49 

    Heroin use is postulated to act as a cofactor in the neuropathogenesis of human immunodeficiency virus (HIV-1) infection. Astrocytes, integral components of the CNS, are reported to be susceptible to HIV-1 infection. Upon activation, astrocytes release a number of immunoregulatory products or...

  • Comparison of peptides in the phloem sap of flowering and non-flowering Perilla and lupine plants using microbore HPLC followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Hoffmann-Benning, Susanne; Gage, Douglas A.; McIntosh, Lee; Kende, Hans; Zeevaart, Jan A. // Planta;Nov2002, Vol. 216 Issue 1, p140 

    Physiological evidence indicates that flower formation is hormonally controlled. The floral stimulus, or florigen, is formed in the leaves as a response to an inductive photoperiod and translocated through the phloem to the apical meristem. However, because of difficulties in obtaining and...

  • Metabolism Study of Veratramine Associated with Neurotoxicity by Using HPLC–MSn. Yue Cong; Jinggong Guo; Zhi Tang; Shuhai Lin; Qingchun Zhang; Jing Li; Zongwei Cai // Journal of Chromatographic Science;Aug2015, Vol. 53 Issue 7, p1092 

    Veratramine (VAM) is the major lipid-soluble alkaloid existing in Veratrum nigrum L. that has been demonstrated to exert neurotoxic effects. To better understand the potential mechanism of neurotoxicity of VAM, VAM-induced DNA damage was measured in the cerebellum and cerebral cortex of mice...

  • Anaylsis of Combinatorial Natural Products by HPLC and CE. Guttman, Andras; Khandurina, Julia // LC-GC North America;Jan2004, Vol. 22 Issue 1, p58 

    Discusses the use of accelerated solvent extraction, high performance liquid chromatography and capillary electrophoresis (CE) to monitor changes in combinatorial genetics-mediated secondary metabolite generation of natural products. Preference of using the CE as a separation method due to...

  • Analysis of Glycosaminoglycans by Electrophoretic Approach. Karousou, Evgenia G.; Viola, Manuela; Vigetti, Davide; Genasetti, Anna; Rizzi, Manuela; Clerici, Moira; Bartolini, Barbara; De Luca, Giancarlo; Passi, Alberto // Current Pharmaceutical Analysis;May2008, Vol. 4 Issue 2, p78 

    Glycosaminoglycans (GAGs) are non branched polysaccharides which are raising a great interest among the scientists for their biological roles. In fact GAGs play a pivotal role in several biological events, since they participate in and regulate cell adhesion, migration and proliferation. The...

  • Simultaneous Identification of Eight Sunscreen Compounds in Cosmetic Products Using High-Performance Liquid Chromatography and Capillary Electrophoresis. Lih-Jeng Juang; Bor-Sen Wang; Huo-Mu Tai; Wei-Jing Hung; Ming-Hsing Huang // Journal of Food & Drug Analysis;Dec2008, Vol. 16 Issue 6, p22 

    p-Aminobenzoates (PABA) and benzophenones in cosmetic products absorb UV radiation. We have developed two simple methods to simultaneously determine whether cosmetic products contain any of eight sunscreen compounds. The first method used high-performance liquid chromatography (HPLC) and a...

  • Capillary Electrophoresis.  // LC-GC North America;Dec2008, Vol. 26 Issue 12, p1156 

    The article offers information on the chromatogrpahy of capillary electrophoresis (CE). It notes that has similar relationship with high performance liquid chromatography (HPLC). Industrial distribution of CE in academic and government institutions record the biggest use of technology, combining...

  • Hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals: developments and applications. Meermann, Björn; Sperling, Michael // Analytical & Bioanalytical Chemistry;Sep2012, Vol. 403 Issue 6, p1501 

    Method development and applications of hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals are summarized within this review. Advantages and limitations of the separation modes-high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics