Identification of Protein Biomarkers in Dupuytren's Contracture using Surface Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF-MS)

O'Gorman, David; Howard, Jeffrey C.; Varallo, Vincenzo M.; Cadieux, Peter; Bowley, Erin; McLean, Kris; Pak, Brian J.; Bing Siang Gan
June 2006
Clinical & Investigative Medicine;Jun2006, Vol. 29 Issue 3, p136
Academic Journal
Background: To study the protein expression profiles associated with Dupuytren's contracture (DC) to identify potential disease protein biomarkers (PBM) using a proteomic technology - Surface Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF-MS). Methods: Normal and disease palmar fascia from DC patients were analyzed using Ciphergen's SELDI-TOF-MS Protein Biological System II (PBSII) ProteinChip® reader. Analysis of the resulting SELDI-TOF spectra was carried out using the peak cluster analysis program (BioMarker Wizard, Ciphergen). Common peak clusters were then filtered using a bootstrap algorithm called SAM (Significant Analysis of Microarrays) for increased fidelity in our analysis. Results: Several differentially expressed low molecular weight (<20 kDa) tissue proteins were identified. Spectra generated using both ZipTipC18 aided Au array and WCX2 array based SELDI-TOF-MS were reproducible, with an average peak cluster mass standard deviation for both methods of <1.74 ×10-4. Initial peak cluster analysis of the SELDI spectra identified both up-(14) and down-(3)regulated proteins associated with DC. Further analysis of the peak cluster data using the bootstrap algorithm SAM identified three disease-associated protein features (4600.8 Da, 10254.5 Da, and 11405.1 Da) that were elevated (5.45, 11.7, and 4.28 fold, respectively, with a 0% median false discovery rate). Conclusion: SELDI-TOF-MS identified three potential low molecular weight tissue protein markers (p4.6DC, p10DC, p11.7DC) for DC. The ability of SELDI-TOF-MS to resolve low molecular weight proteins suggests that the method may provide a means of deciphering the biomarker-rich low molecular weight region of the human proteome. Application of such novel technology may help clinicians to focus on specific molecular abnormalities in diseases with no known molecular pathogenesis, and uncover therapeutic and/or diagnostic targets.


Related Articles

  • Optimization and evaluation of surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry for protein profiling of cerebrospinal fluid. Guerreiro, Nelson; Gomez-Mancilla, Baltazar; Charmont, Stéphane // Proteome Science;2006, Vol. 4, p7 

    Cerebrospinal fluid (CSF) potentially carries an archive of peptides and small proteins relevant to pathological processes in the central nervous system (CNS) and surrounding brain tissue. Proteomics is especially well suited for the discovery of biomarkers of diagnostic potential in CSF for...

  • Biomarker discovery in MALDI-TOF serum protein profiles using discrete wavelet transformation. Theodore Alexandrov; Jens Decker; Bart Mertens; Andre M. Deelder; Rob A. E. M. Tollenaar; Peter Maass; Herbert Thiele // Bioinformatics;Mar2009, Vol. 25 Issue 5, p643 

    Motivation: Automatic classification of high-resolution mass spectrometry proteomic data has increasing potential in the early diagnosis of cancer. We propose a new procedure of biomarker discovery in serum protein profiles based on: (i) discrete wavelet transformation of the spectra; (ii)...

  • SELDI Protein Chip Technology for the Detection of Serum Biomarkers for Liver Disease. Lei Chen; Fatima, Sarwat; Jirun Peng; Xisheng Leng // Protein & Peptide Letters;May2009, Vol. 16 Issue 5, p467 

    The need to identify disease biomarkers is critical to ensure fast diagnosis and timely treatment. Surface enhanced laser desorption/ionization time-of-fight mass spectrometry (SELDI-TOF-MS) is a widely used technology platform for diagnostic biomarker discovery. This short review provides an...

  • Protein Biomarkers in Serum of Patients with Schizophrenia. Ding, Yin-huan; Guo, Jian-hong; Hu, Qiong-ying; Jiang, Wei; Wang, Kai-zheng // Cell Biochemistry & Biophysics;Jul2015, Vol. 72 Issue 3, p799 

    This study was devised to identify potential biomarkers of schizophrenia (SP) using proteomics techniques. We obtained 44 serum specimens from patients with SP, 26 specimens from patients with depression, and 40 specimens from healthy controls. Immobilized metal affinity capture protein chips...

  • Protein Markers for the Differential Diagnosis of Vascular Dementia and Alzheimer's Disease. Simonsen, A. H.; Hagnelius, N.-O.; Waldemar, G.; Nilsson, T. K.; McGuire, J. // International Journal of Proteomics;2012, p1 

    Alzheimer's disease (AD) is the most common form of dementia found in all human populations worldwide, while vascular dementia (VaD) is the second most common form of dementia. New biomarkers for early and specific diagnosis of AD and VaD are needed to achieve greater insight into changes...

  • Metabolomics Discloses Potential Biomarkers for the Noninvasive Diagnosis of Idiopathic Portal Hypertension. Seijo, Susana; Lozano, Juan Jose; Alonso, Cristina; Reverter, Enric; Miquel, Rosa; Abraldes, Juan G; Martinez-Chantar, Maria Luz; Garcia-Criado, Angeles; Berzigotti, Annalisa; Castro, Azucena; Mato, Jose M; Bosch, Jaume; Garcia-Pagan, Juan Carlos // American Journal of Gastroenterology;Jun2013, Vol. 108 Issue 6, p926 

    OBJECTIVES:Idiopathic portal hypertension (IPH) is a rare cause of portal hypertension that lacks a specific diagnostic test. Requiring ruling-out other causes of portal hypertension it is frequently misdiagnosed. This study evaluates whether using high-throughput techniques there is a...

  • Trigger finger may not be all it seems. Lackey, Emma; Sutton, Ron // GP: General Practitioner;10/20/2003, p73 

    Trigger finger is often seen as a Monday morning diagnosis, because symptoms can flare up after a weekend of unaccustomed activity, such as heavy pruning or DIY work. The condition involves the clicking, locking or snapping of a finger at the metacarpophalangeal joint. Patients with diabetes,...

  • MALDI-TOF-MS Screens for Diagnostic Biomarkers.  // Fertility Weekly;2/3/2014, p12 

    The article discusses the study "Evaluation of Novel Serum Biomarkers and the Proteomic Differences of Endometriosis and Adenomyosis Using MALDI-TOF-MS," by Xiaoyu Long and colleagues, published in the 2013 issue of "Archives of Gynecoloy and Obstetrics." The study assessed the efficiency of...

  • New serum biomarkers for detection of esophageal carcinoma using Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Guo, Renyong; Pan, Chunqin; Shen, Jianmin; Liu, Chibo // Journal of Cancer Research & Clinical Oncology;Mar2011, Vol. 137 Issue 3, p513 

    Purpose: To screen for the potential protein biomarkers in serum for the diagnosis of esophageal carcinoma (EC) using proteomic fingerprint technology. Methods: Proteomic fingerprint technology combining magnetic beads with MALDI-TOF-MS was used to profile and compare the serum proteins from 78...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics