TITLE

The maize heat shock factor-binding protein paralogs EMP2 and HSBP2 interact non-redundantly with specific heat shock factors

AUTHOR(S)
Suneng Fu; Rogowsky, Peter; Nover, Lutz; Scanlon, Michael
PUB. DATE
July 2006
SOURCE
Planta;Jul2006, Vol. 224 Issue 1, p42
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The heat shock response (HSR) is a conserved mechanism by which transcripts of heat shock protein ( hsp) genes accumulate following mobilization of heat shock transcription factors (HSFs) in response to thermal stress. Studies in animals identified the heat shock factor-binding protein1 (HSBP1) that interacts with heat shock transcription factor1 (HSF1) during heat shock attenuation; overexpression analyses revealed that the coiled-coil protein HSBP1 functions as a negative regulator of the HSR. Zea mays contains two HSBP paralogs, EMP2 and HSBP2, which exhibit differential accumulation during the HSR and plant development. Embryo-lethal recessive emp2 mutations revealed that EMP2 is required for the down-regulation of hsp transcription during embryogenesis, whereas accumulation of HSBP2 is induced in seedlings following heat shock. Notwithstanding, no interaction has yet been demonstrated between a plant HSBP and a plant HSF. In this report 22 maize HSF isoforms are identified comprising three structural classes: HSF-A, HSF-B and HSF-C. Phylogenetic analysis of Arabidopsis, maize and rice HSFs reveals that at least nine ancestral HSF isoforms were present prior to the separation of monocot and eudicots, followed by differential amplification of HSF members in these lineages. Yeast two-hybrid analyses show that EMP2 and HSBP2 interact non-redundantly with specific HSF-A isoforms. Site-specific mutagenesis of HSBP2 reveals that interactions between hydrophobic residues within the coiled coil are required for HSF::HSBP2 binding; domain swapping demonstrate that the isoform specificity of HSF::HSBP interaction is conferred by residues outside of the coiled coil. These data suggest that the non-redundant functions of the maize HSBPs may be explained, at least in part, by the specificity of HSBP::HSF interactions during plant development.
ACCESSION #
20925090

 

Related Articles

  • Genome-wide identification, classification and analysis of heat shock transcription factor family in maize. Yong-Xiang Lin; Hai-Yang Jiang; Zhang-Xin Chu; Xiu-Li Tang; Su-Wen Zhu; Bei-Jiu Cheng // BMC Genomics;2011, Vol. 12 Issue 1, p76 

    Background: Heat shock response in eukaryotes is transcriptionally regulated by conserved heat shock transcription factors (Hsfs). Hsf genes are represented by a large multigene family in plants and investigation of the Hsf gene family will serve to elucidate the mechanisms by which plants...

  • HSFs and regulation of Hsp70.1 (Hspa1b) in oocytes and preimplantation embryos: new insights brought by transgenic and knockout mouse models. Masson, Florent; Christians, Elisabeth // Cell Stress & Chaperones;Autumn2011, Vol. 16 Issue 3, p275 

    Gene encoding heat shock protein (Hsps) are induced following a thermal stress thanks to the activation of heat shock transcription factor (HSF) which interacts with heat shock elements (HSE) located within the sequence of Hsp promoters. This cellular and protective response (heat shock response...

  • Control of somatic embryogenesis and embryo development by AP2 transcription factors. El Ouakfaoui, Souad; Schnell, Jaimie; Abdeen, Ashraf; Colville, Adam; Labbé, Hélène; Han, Shuyou; Baum, Bernard; Laberge, Serge; Miki, Brian // Plant Molecular Biology;Nov2010, Vol. 74 Issue 4/5, p313 

    Members of the AP2 family of transcription factors, such as BABY BOOM ( BBM), play important roles in cell proliferation and embryogenesis in Arabidopsis thaliana ( AtBBM) and Brassica napus ( BnBBM) but how this occurs is not understood. We have isolated three AP2 genes ( GmBBM1, GmAIL5,...

  • An Arabidopsis gene encoding a C2H2-domain protein with alternatively spliced transcripts is essential for endosperm development. Lu, Xiaoduo; Li, Yuan; Su, Yanping; Liang, Qiuju; Meng, Hongyan; Li, Sha; Shen, Songdong; Fan, Yunliu; Zhang, Chunyi // Journal of Experimental Botany;Nov2012, Vol. 63 Issue 16, p5935 

    The endosperm is an essential part of the seed which sustains embryo development and contains storage reserves. Endosperm development involves a series of nuclear divisions in the absence of cytokinesis. In this study, it is shown that a mutant Arabidopsis plant carrying the disrupted At4g24900...

  • Evaluation of the genotoxic or mutagenic effects of thermal stress on cultured human lymphocytes. İLA, Hasan Basri; TOPAKTAŞ, Mehmet; ARSLAN, Mehmet; BÜYÜKLEYLA, Mehmet; İSTİFLİ, Erman Salih // Turkish Journal of Biology;2015, Vol. 39 Issue 1, p98 

    This study was performed to determine the cytogenetic effects of short-term thermal stress in human cultured lymphocytes. Experimental heat shock (39 °C) was performed alone or with the addition of mitomycin C (MMC) to determine the synergistic or antagonistic effects of heat shock on...

  • Arabidopsis thaliana PDX1.2 is critical for embryo development and heat shock tolerance. Leuendorf, Jan; Mooney, Sutton; Chen, Liyuan; Hellmann, Hanjo // Planta;Jul2014, Vol. 240 Issue 1, p137 

    Main conclusion: PDX1.2 is expressed in the basal part of the globular-stage embryo, and plays critical roles in development, hypocotyl elongation, and stress response. Abstract: The Arabidopsis thaliana PDX1.2 protein belongs to a small family of three members. While PDX1.1 and PDX1.3 have been...

  • The 26S Proteasome Function and Hsp90 Activity Involved in the Regulation of HsfA2 Expression in Response to Oxidative Stress. Nishizawa-Yokoi, Ayako; Tainaka, Hitoshi; Yoshida, Eriko; Tamoi, Masahiro; Yabuta, Yukinori; Shigeoka, Shigeru // Plant & Cell Physiology;Mar2010, Vol. 51 Issue 3, p486 

    Heat shock transcription factor A2 (HsfA2) is induced under environmental stress and regulates transcription of various defense-related genes. Thus HsfA2 plays an important role in induction of defenses against different types of environmental stress, but its mode of regulation remains unknown....

  • JAGGED LATERAL ORGAN ( JLO) controls auxin dependent patterning during development of the Arabidopsis embryo and root. Bureau, Marina; Rast, Madlen; Illmer, Jasmin; Simon, Rüdiger // Plant Molecular Biology;Nov2010, Vol. 74 Issue 4/5, p479 

    The plant hormone auxin plays a role in virtually every aspect of plant growth and development. Temporal and spatial distribution of auxin largely depends on the dynamic expression and subcellular localization of the PIN auxin-efflux carrier proteins. We show here that the Arabidopsis thaliana...

  • Genome-wide identification and characterization of R2R3MYB family in Solanum lycopersicum. Zhao, Panpan; Li, Qiang; Li, Jing; Wang, Lina; Ren, Zhonghai // Molecular Genetics & Genomics;Dec2014, Vol. 289 Issue 6, p1183 

    The R2R3MYB proteins comprise one of the largest families of transcription factors and play regulatory roles in developmental processes and defense responses in plants. However, there has been relatively little effort to systematically carry out comprehensive genomic and functional analyses of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics