TITLE

Degradation of ureidoglycolate in French bean ( Phaseolus vulgaris) is catalysed by a ubiquitous ureidoglycolate urea-lyase

AUTHOR(S)
Muñoz, Alfonso; Raso, María José; Pineda, Manuel; Piedras, Pedro
PUB. DATE
July 2006
SOURCE
Planta;Jul2006, Vol. 224 Issue 1, p175
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A ureidoglycolate-degrading activity was analysed in different tissues of French bean ( Phaseolus vulgaris L.) plants during development. Activity was detected in all the tissues analysed, although values were very low in seeds before germination and in cotyledons. After radicle emergence, the activity increased due to high activity present in the axes. The highest levels of specific activity were found in developing fruits, from which the enzyme was purified and characterised. This is the first ureidoglycolate-degrading activity that has been purified to homogeneity from a ureide legume. The enzyme was purified 280 fold, and the specific activity for the pure enzyme was 4.4 units mg−1, which corresponds to a turnover number of 1,055 min−1. The native enzyme has a molecular mass of 240 kDa and consists of six identical or similar-sized subunits each of 38 kDa. The activity of the purified enzyme was completely dependent on manganese and asparagine. The enzyme exhibited hyperbolic, Michaelian kinetics for ureidoglycolate with a K m value of 3.9 mM. This enzyme has been characterised as a ureidoglycolate urea-lyase (EC 4.3.2.3).
ACCESSION #
20925089

 

Related Articles

  • Effects of cryopreservation of Phaseolus vulgaris L. seeds on early stages of germination. Cejas, Inaudis; Vives, Karel; Laudat, Taletha; González-Olmedo, Justo; Engelmann, Florent; Martínez-Montero, Marcos; Lorenzo, José // Plant Cell Reports;Nov2012, Vol. 31 Issue 11, p2065 

    In this work, we studied the effects of cryopreservation on various parameters of early stages of germination of Phaseolus vulgaris seeds (0, 7 and 14 days). Percentages of germination, fresh mass of different plant parts, levels of chlorophyll pigments ( a, b, total), malondialdehyde, other...

  • AVALIAÇÃO DE DANOS MECÂNICOS EM SEMENTES DE FEIJÂO POR MEIO DA ANÁLISE DE IMAGENS. VAZ MONDO, VITOR HENRIQUE; GOMES JUNIOR, FRANCISCO GUILHIEN; PUPIM, TÚLIO LOURENÇO; CICERO, SILVIO MOURE // Revista Brasileira de Sementes;2009, Vol. 31 Issue 2, p27 

    Bean seed (Phaseolus vulgaris L.) quality is greatly affected by harvesting, drying, handling and storage activities during the seed production process. In these operations, seeds collide repeatedly against metal surfaces predisposing them to mechanical damage. In this context, this...

  • Copper Affects the Cotyledonary Carbohydrate Status During the Germination of Bean Seed. Sfaxi-Bousbih, Amira; Chaoui, Abdelilah; Ferjani, Ezzedine // Biological Trace Element Research;Oct2010, Vol. 137 Issue 1, p110 

    Seeds of bean ( Phaseolus vulgaris L.) were germinated by soaking in distilled water or copper chloride solution. The relationships among copper excess treatment, germination rate, dry weight, sugar contents, and carbohydrase activities in cotyledon were investigated. Heavy metal stress provoked...

  • Effects of copper on reserve mobilization in embryo of Phaseolus vulgaris L. Karmous, Inès; Bellani, Lorenza; Chaoui, Abdelilah; Ferjani, Ezzedine; Muccifora, Simonetta // Environmental Science & Pollution Research;Jul2015, Vol. 22 Issue 13, p10159 

    The present research reports a biochemical and micro-submicroscopic analysis of copper effect on reserve mobilization during germination of Phaseolus vulgaris L. var. soisson nain hatif seeds. Dry embryonic cells are rich in protein bodies and little starch grains. In Cu-treated embryos copper...

  • Some Seed Traits and Their Relationships to Seed Germination, Emergence Rate Electrical Conductivity in Common Bean (Phaseolus vulgaris L.). Borji, M.; Ghorbanli, M.; Sarlak, M. // Asian Journal of Plant Sciences;2007, Vol. 6 Issue 5, p781 

    The size of the seeds, shape index, shape of the seeds, 100-seed-weight, germination and emergence tests, the values of the test a rates, the speed of the imbibition and electrical conductivity were determined in 10 common bean cultivars in two group of red and white bean (Phaseolus vulgaris...

  • Dormancy as exaptation to protect mimetic seeds against deterioration before dispersal. Brancalion, Pedro H. S.; Novembre, Ana D. L. C.; Rodrigues, Ricardo R.; Filho, Júlio Marcos // Annals of Botany;Jun2010, Vol. 105 Issue 6, p991 

    Background and Aims: Mimetic seeds simulate the appearance of fleshy fruits and arilled seeds without producing nutritive tissues as a reward for seed dispersers. In this strategy of seed dispersal, seeds may remain attached to the mother plant for long periods after maturity, increasing their...

  • PLANT PHYSIOLOGY: Toughening up soft centres. Surridge, Christopher // Nature Reviews Molecular Cell Biology;Apr2002, Vol. 3 Issue 4, p228 

    Discusses research being done on the role of pectate lyase in the ripening of fruits. Reference to a study by S. Jim�nez-Berm�dez et al published in the 2002 issue of "Plant Physiology"; Investigation of the activity of pectate lyases in hemi-cellulose pecting in plant cell walls;...

  • ZmLrk-1, a receptor-like kinase induced by fungal infection in germinating seeds. Royo, Joaquín; Gómez, Elisa; Balandín, Maite; Muñiz, Luis M.; Hueros, Gregorio // Planta;Jun2006, Vol. 223 Issue 6, p1303 

    We report here on the identification and characterization of ZmLrk-1, a member of the Lrk class of receptor-like kinases in Zea mays. This gene was found to be located at the bin21.40 region on the short arm of maize chromosome 8, closely linked to the previously reported pseudogene of the same...

  • HPLC analyses of seed storage proteins reveal polymorphism in Italian common bean (Phaseolus vulgaris L.) ecotypes. Mennella, Giuseppe; Onofaro Sanajà, Vincenzo; D'Alessandro, Antonietta; Milone, Marco; Perrone, Domenico // Euphytica;Oct2003, Vol. 134 Issue 1, p85 

    Phaseolin (PHAS) and phytohemagglutinin (PHA) represent the main seed protein fractions in common bean. Currently, several ecotypes of common bean cultivated in marginal areas exist, above all in Southern Italy regions. Such germplasm could be lost due to the genetic uniformity of new cultivars....

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics