TITLE

Cytokine measurement in lymphocyte culture supernatant of inactive lepromatous leprosy patients

AUTHOR(S)
Fulya, I.; Mehmet, O.; Handan, A.; Vedat, B.
PUB. DATE
April 2006
SOURCE
Indian Journal of Medical Microbiology;Apr2006, Vol. 24 Issue 2, p121
SOURCE TYPE
Academic Journal
DOC. TYPE
journal article
ABSTRACT
The aim of the present study was to determine the effects of stimulation of sonicated Mycobacterium leprae (MLS) extract and phorbol myristate acetate (PMA) on the pattern of cytokine production in peripheral blood mononuclear cells (PBMC) and to find out whether there is any difference between stimulation of MLS extract and PMA. Blood samples were collected and PBMC isolated from 43 inactive lepromatous leprosy patients. After culture for 24 hours, lymphocytes were stimulated with MLS extract and PMA. In the culture supernatant, IL-2, 4, 6, 8, TNF-alpha and TGF-beta levels were measured by using ELISA. M. leprae stimulated group IL-6, IL-8, TNF-alpha, TGF-beta levels were found significantly higher than PMA stimulated group (P < 0.05). However, there was no difference between the two groups for IL-4. Only IL-2 levels were higher in PMA stimulated group than M. leprae stimulated group. Sonicated M. leprae extract have a strong effect on cytokine levels in vitro. Our results suggest that antigens with varying specificities favour the production of distinct cytokine patterns following in vitro restimulation.
ACCESSION #
20913742

 

Related Articles

  • The Study of Mycobacterium leprae Infection in Interferon-γ Gene-Disrupted Mice as a Model to Explore the Immunopathologic Spectrum of Leprosy. Adams, Linda B.; Scollard, David M.; Ray, Nashone A.; Cooper, Andrea M.; Frank, Anthony A.; Orme, Ian M.; Krahenbuhl, James L. // Journal of Infectious Diseases;2/15/2002 Supplement, Vol. 185, pS1 

    Mycobacterium leprae infection was evaluated in interferon-γ knockout (GKO) mice. At 4 months, growth of the bacilli in the footpads of GKO mice plateaued a log[sub 10] higher than that in control mice. Control mice exhibited mild lymphocytic and histiocytic infiltrates, whereas GKO mice...

  • Thousands of Years Later -- a New Kind of Leprosy. Kemper, Carol A.; Price, Connie // Travel Medicine Advisor;Feb2009, Vol. 19 Issue 2, p11 

    A reprint of the article "Thousand of Years Later -- A New Kind of Leprosy," by Carol A. Kemper, which appeared in the January 2009 issue of "Infectious Disease Alert" is presented. The article focuses on two cases of diffuse lepromatous leprosy (DLL), an especially virulent form of...

  • Molecular typing of Mycobacterium leprae strains from northern India using short tandem repeats. Lavania, Mallika; Katoch, Kiran; Sharma, Rahul; Sharma, Pragya; Das, Ram; Gupta, Anuj Kumar; Chauhan, D. S.; Katoch, V. M. // Indian Journal of Medical Research;Jun2011, Vol. 133 Issue 6, p618 

    Background & objectives: Due to the inability to cultivate Mycobacterium leprae in vitro and most cases being paucibacillary, it has been difficult to apply classical genotyping methods to this organism. The objective of this study was therefore, to analyze the diversity among M. leprae strains...

  • Leprosy. Howie-Willis, Ian // Encyclopaedia of Aboriginal Australia;1994, Vol. 1, p616 

    An encyclopedia entry for leprosy in Aboriginal Australia is presented. Leprosy, also known as Hansen's disease, is a chronic ailment caused by the Mycobacterium leprae bacillus. It is stated that leprosy in Australia has largely been a tropical disease, and the great majority of its sufferers...

  • New Insights into the Geographic Distribution of Mycobacterium leprae SNP Genotypes Determined for Isolates from Leprosy Cases Diagnosed in Metropolitan France and French Territories. Reibel, Florence; Chauffour, Aurélie; Brossier, Florence; Jarlier, Vincent; Cambau, Emmanuelle; Aubry, Alexandra // PLoS Neglected Tropical Diseases;10/6/2015, Vol. 9 Issue 10, p1 

    Background: Between 20 and 30 bacteriologically confirmed cases of leprosy are diagnosed each year at the French National Reference Center for mycobacteria. Patients are mainly immigrants from various endemic countries or living in French overseas territories. We aimed at expanding data...

  • Evaluation of Cellular Phenotypes Implicated in Immunopathogenesis and Monitoring Immune Reconstitution Inflammatory Syndrome in HIV/Leprosy Cases. Giacoia-Gripp, Carmem Beatriz Wagner; Sales, Anna Maria; Costa Nery, José Augusto da; Santos-Oliveira, Joanna Reis; Oliveira, Ariane Leite de; Sarno, Euzenir Nunes; Morgado, Mariza Gonçalves // PLoS ONE;2011, Vol. 6 Issue 12, p1 

    Background: It is now evident that HAART-associated immunological improvement often leads to a variety of new clinical manifestations, collectively termed immune reconstitution inflammatory syndrome, or IRIS. This phenomenon has already been described in cases of HIV coinfection with...

  • Antigenic heterogeneity in patients with reactions in borderline leprosy. Barnetson, R. StC.; Bjune, G.; Pearson, J.M.H.; Kronvall, G. // British Medical Journal;11/22/1975, Vol. 4 Issue 5994, p435 

    Investigates the antigenic heterogeneity on patients with reactions in borderline leprosy. Increase of lymphocyte transformation (LT) on patients; Use of mycobacterium leprae as antigen; Details on the ratios of the LT test results.

  • The History and Mystery of Leprosy. Cory, David A. // History Magazine;Dec2008/Jan2009, Vol. 10 Issue 2, p16 

    The article provides information on the history and mystery of leprosy. It states the elimination of the terms leprosy and leper, and the use of the term Hansen's disease which recognizes the contributions of Gerhard Armauer Hansen, who discovered that leprosy is caused by Mycobacterium leprae....

  • Differential Dermal Expression of CCL17 and CCL18 in Tuberculoid and Lepromatous Leprosy. Berrington, William R.; Kunwar, Chhatra B.; Neupane, Kapil; van den Eeden, Susan J. F.; Jr.Vary, James C.; Peterson, Glenna J.; Wells, Richard D.; Geluk, Annemieke; Hagge, Deanna A.; Hawn, Thomas R. // PLoS Neglected Tropical Diseases;Nov2014, Vol. 8 Issue 11, p1 

    Background: Leprosy is characterized by polar clinical, histologic and immunological presentations. Previous immunologic studies of leprosy polarity were limited by the repertoire of cytokines known at the time. Methodology: We used a candidate gene approach to measure mRNA levels in skin...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics