TITLE

Chlamydia trachomatis antigen detection in pregnancy and its verification by antibody blocking assay

AUTHOR(S)
Malenie, R.; Joshi, P. J.; Mathur, M. D.
PUB. DATE
April 2006
SOURCE
Indian Journal of Medical Microbiology;Apr2006, Vol. 24 Issue 2, p97
SOURCE TYPE
Academic Journal
DOC. TYPE
journal article
ABSTRACT
Purpose: To detect the prevalence of genital infection caused by Chlamydia trachomatis in pregnant women and also to confirm the positive results using blocking antibody assay.Methods: Endocervical specimens were collected from 200 symptomatic and asymptomatic pregnant women attending the ANC OPD at M P Shah Medical College, Jamnagar. The samples were tested for presence of Chlamydia trachomatis antigen using the monoclonal antibody. Blocking antibody assay was used to further verify the positive results.Results: Out of 200 pregnant women, 38 (19%) were found positive for Chlamydia trachomatis antigen. Out of the 68 symptomatic patients, C. trachomatis antigen was detected in 26.4%. After verification of the positive samples 13.6% of the asymptomatic pregnant women were found to be harbouring the infection in their genital tract. Two (5.2%) out of the 38 positive samples, on verification with the blocking antibody assay, were found to be false positive by IDEIA,TM thus the specificity of the IDEIATM being 94.8%. In patients with previous history of abortions, 27.7% were tested positive for C. trachomatis infection.Conclusions: Significant number of pregnant women shad C. trachomatis antigen in their endocervical canal, which can be easily diagnosed by this simple enzyme immuno assay having a specificity of 94.8%. Verification of positive results by antibody blocking assay can further improve the specificity of this non-culture test. Asymptomatic patients should also be screened for the infection. History of previous abortions places the patient at a higher risk for C. trachomatis infection thus such patients should be definitely tested for chlamydia infection.
ACCESSION #
20913737

 

Related Articles

  • COMPARISON OF VULVAL TO CERVICAL SPECIMENS FOR CHLAMYDIA TRACHOMATIS LCR DIAGNOSIS. O'Connor, C.; Heffernan, C.; FitzGerald, P.; Barron, D. // Sexually Transmitted Infections;Jun2003 Supplement 1, Vol. 79, pA13 

    Background: A suitable specimen collection method for ligase chain reaction (LCR) testing for Chlamydia trachomatis was being considered for a community setting. The options were (a) first void urine (FVU); (b) cervical specimens; or (c) vulval specimens. FVUs were difficult and expensive to...

  • ASSESSMENT OF THE SENSITIVITY OF URINE SAMPLE TESTING FOR CHLAMYDIA INFECTION IN WOMEN PRESENTING FOR TERMINATION OF PREGNANCY. Wilkinson, H.; Ivens, D. // Sexually Transmitted Infections;Jun2003 Supplement 1, Vol. 79, pA13 

    Objectives: To determine the sensitivity of urine samples compared with endocervical swabs for the detection of Chlamydia trachomatis infection in a termination of pregnancy (TOP) assessment clinic at the Royal Free Hampstead NHS Trust. To compare the results with samples taken from non-pregnant...

  • Chlamydia trachomatis infections in pregnancy. Korn, Abner P. // Contemporary OB/GYN;Apr2000, Vol. 45 Issue 4, p65 

    Discusses Chlamydia trachomatis infections in pregnancy. Risk factors; Diagnosis; Treatment.

  • Is there any role for rapid tests for Chlamydia trachomatis? Rani, Ranjana; Corbitt, Gerald; Killough, Rosemary; Curless, Eric // International Journal of STD & AIDS;Jan2002, Vol. 13 Issue 1, p22 

    The main aim of this pilot study was to evaluate the sensitivity and specificity of a recently introduced rapid diagnostic test, the QuickVue chlamydia test against polymerase chain reaction (PCR) for endocervical samples. Due to concerns surrounding the low specificity of rapid tests for a low...

  • Chlamydia trachomatis: How to screen and treat it economically.  // Modern Medicine;May94, Vol. 62 Issue 5, p64 

    Presents an abstract of the article entitled, `An economic evaluation of screening for Chlamydia trachomatis in adolescent males,' by M. Genc, L. Ruusuvaara and P.A. Mardh as published in the periodical `Journal of the American Medical Association' dated November 3, 1993.

  • Chlamydia trachomatis: How to screen and treat it economically.  // Modern Medicine;May94, Vol. 62 Issue 5, p64 

    Presents an abstract of the article entitled, `Evaluation of urine-based screening strategies to detect Chlamydia trachomatis among sexually active asymptomatic young males,' by M.A. Shafer, J. Schachter, J. Moncada et al as published in the periodical `Journal of the American Medical...

  • Chlamydia trachomatis: How to screen and treat it economically.  // Modern Medicine;May94, Vol. 62 Issue 5, p64 

    Presents an abstract of the article entitled, `Long-term eradication of Chlamydia trachomatis genital infection after antimicrobial therapy: Evidence against persistent infection,' by K.A. Workowski, M.F. Lampe, K.G. Wong et al as published in the periodical `Journal of the American Medical...

  • Prescribing for partners: Exploring the questions for women in pregnancy with positive Chlamydia Trachomatis. Gray, Elaine // Midwifery News;Jun2010, Issue 57, p14 

    The article focuses on chlamydia trachomatis in pregnant women. It states that chlamydia trachomatis is the most frequent sexually transmissible infection (STI) in the world and the most common curable STI in New Zealand. The New Zealand College of Midwives (NZCOM) consensus statement on...

  • Controversy: Should there be home tests for sexual diseases? Phillips, Lynn // Glamour;Feb95, Vol. 93 Issue 2, p90 

    Reports on the collaboration of National Institutes of Health scientists with researchers and manufacturers to develop simple and inexpensive diagnostic kits for gonorrhea and chlamydia. Rockefeller Foundation's offer of a prize to the company that creates the first kit; Controversies over home...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics