Absence of β -Catenin Alteration in Hepatic Tumors Induced by p-Nitroanisole in Crj:BDF1 Mice

Kushida, Masahiko; Aiso, Sigetoshi; Morimura, Keiichirou; Min Wei; Wanibuchi, Hideki; Nagano, Kasuke; Fukushima, Shoji
May 2006
Toxicologic Pathology;2006, Vol. 34 Issue 3, p237
Academic Journal
In the present study, β -catenin localization in hepatocellular neoplasms and hepatoblastomas, induced by oral administration of p-Nitroanisole (pNA) in Crj:BDF1 for 2 years, was evaluated by immunohistochemistry along with genetic alterations in exon 2 of β -catenin by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) approach. Genomic DNA was isolated from paraffin sections of a total of 53 liver tumors. Immunohistochemical analysis revealed no abnormal accumulation of the β -catenin protein in any of the cases. No mutations (0/13), 20% silent mutations (3/15) and 8% silent plus 12% functional mutations (2 + 3/25), not in the multiple phosphorylation sites of β -catenin, were observed in hepatocellular adenomas, carcinomas and hepatoblastomas, respectively. The results indicate that β -catenin does not play an important role in development of hepatic tumors induced by pNA in Crj:BDF1 mice.


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