Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes

Dita, Miguel A.; Rispail, Nicolas; Prats, Elena; Rubiales, Diego; Singh, Karam B.
January 2006
Euphytica;Jan2006, Vol. 147 Issue 1/2, p1
Academic Journal
Biotic and abiotic stresses cause significant yield losses in legumes and can significantly affect their productivity. Biotechnology tools such as marker-assisted breeding, tissue culture, in vitro mutagenesis and genetic transformation can contribute to solve or reduce some of these constraints. However, only limited success has been achieved so far. The emergence of “omic” technologies and the establishment of model legume plants such as Medicago truncatula and Lotus japonicus are promising strategies for understanding the molecular genetic basis of stress resistance, which is an important bottleneck for molecular breeding. Understanding the mechanisms that regulate the expression of stress-related genes is a fundamental issue in plant biology and will be necessary for the genetic improvement of legumes. In this review, we describe the current status of biotechnology approaches in relation to biotic and abiotic stresses in legumes and how these useful tools could be used to improve resistance to important constraints affecting legume crops.


Related Articles

  • Developing genetic reagents to facilitate recovery, analysis, and maintenance of mouse mutations. Rinchik, Eugene M. // Mammalian Genome;Jul2000, Vol. 11 Issue 7, p489 

    Because the mouse has become the pre-eminent model system for functional genomics and analysis of complex-systems/pathways in mammals, there has been an escalation of interest in the generation and analysis of mouse mutations to use as tools in these analyses. I argue here for a parallel...

  • Genetic studies in Centrosema pubescens benth, a tropical forage legume: the mating system, genetic variability and genetic relationships between Centrosema species. Sousa, A.; Carvalho, M.; Ramos, A.; Campos, T.; Sforça, D.; Zucchi, M.; Jank, L.; Souza, A. // Euphytica;Sep2011, Vol. 181 Issue 2, p223 

    In this study, we used microsatellite loci to estimate the outcrossing rate of Centrosema pubescens in open-pollinated populations of 10 progenies that each contained 20 genotypes. The multilocus outcrossing rate was 27%, which suggested a mixed mating system with a predominance of autogamy. The...

  • Cathepsin K gene mutations and 1q21 haplotypes in patients with pycnodysostosis in an outbred population. Haagerup, Annette; Hertz, Jens M; Christensen, Mogens F; Binderup, Helle; Kruse, Torben A // European Journal of Human Genetics;Jun2000, Vol. 8 Issue 6, p431 

    The molecular genetics of the autosomal recessive disorder pycnodysostosis was studied in five independent families from an outbred Caucasian population. We found two new mutations and one recently described mutation in the cathepsin K gene by sequencing DNA from eight patients with...

  • EYA1 nonsense mutation in a Japanese branchio-oto-renal syndrome family. Usami, S.; Abe, Satoko; Shinkawa, Hideichi; Deffenbacher, Karen; Kumar, Shrawan; Kimberling, William J. // Journal of Human Genetics;1999, Vol. 44 Issue 4, p261 

    Abstract Advances in molecular genetics have recently revealed that mutations in the EYA1 gene are responsible for branchio-oto-renal (BOR) syndrome in European and other populations. This is the first report confirming that an EYA1 gene mutation is also disease-causing in an Asian population....

  • Analytical Description of Mutational Effects in Competing Asexual Populations. Pinkel, Daniel // Genetics;Dec2007, Vol. 177 Issue 4, p2135 

    The adaptation of a population to a new environment is a result of selection operating on a suite of stochastically occurring mutations. This article presents an analytical approach to understanding the population dynamics during adaptation, specifically addressing a system in which periods of...

  • Gene conversion is a likely cause of mutation in PKD1. Watnick, Terry J.; Gandolph, Michael A.; Weber, Horst; Neumann, Hartmut P. H.; Germino, Gregory G. // Human Molecular Genetics;Aug98, Vol. 7 Issue 8, p1239 

    Approximately 70% of the gene responsible for the most common form of autosomal dominant polycystic kidney disease (PKD1) is replicated in several highly homologous copies located more proximally on chromosome 16. We recently have described a novel technique for mutation detection in the...

  • A transgenic mouse assay for agouti protein activity. Perry, William L.; Hustad, Carolyn M. // Genetics;May95, Vol. 140 Issue 1, p267 

    Presents a transgenic mouse assay for agouti protein activity. Expression of agouti during the middle part of the hair growth cycle in wild-type mice; Molecular analysis of agouti alleles; Agouti expression driven by the human beta-ACTIN promoter.

  • Mutation detection and single-molecule counting using isothermal rolling-circle amplification. Lizardi, Paul M.; Huang, Xiaohua; Zhu, Zhengrong; Bray-Ward, Patricia; Thomas, David C.; Ward, David C. // Nature Genetics;Jul98, Vol. 19 Issue 3, p225 

    Rolling-circle amplification (RCA) driven by DNA polymerase can replicate circularized oligonucleotide probes with either linear or geometric kinetics under isothermal conditions. In the presence of two primers, one hybridizing to the + strand, and the other, to the ? strand of DNA, a complex...

  • PLANT DEVELOPMENT: De-pipping the Pippin. Surridge, Christopher // Nature Reviews Molecular Cell Biology;Mar2001, Vol. 2 Issue 3, p162 

    Discusses research being done on mutations in a single gene that result in seedless apples. Reference to a study by Jia-Long Yao et al published in the 2001 issue of the "Proceedings of the National Academy of Sciences"; Varieties of seedless apple; Description of the pistillata gene.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics