TITLE

Induction of Fibroblast Growth Factor-9 and Interleukin-1α Gene Expression by Motorcycle Exhaust Particulate Extracts and Benzo(a)pyrene in Human Lung Adenocarcinoma Cells

AUTHOR(S)
Tzuu-Huei Ueng; Chia-Chi Hung; Min-Liang Kuo; Ping-Kun Chan; Shih-Hsiung Hu; Pan-Chyr Yang; Chang, Louis W.
PUB. DATE
October 2005
SOURCE
Toxicological Sciences;Oct2005, Vol. 87 Issue 2, p483
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Motorcycle exhaust particulates (MEP) contain carcinogenic polycyclic aromatic hydrocarbons including benzo(a)pyrene. This study has determined the ability of MEP to alter the expression of select genes from drug metabolism, cytokine, oncogene, tumor suppressor, and estrogen signaling families of human lung adenocarcinoma CL5 cells. cDNA microarray analyses and confirmation studies were performed using CL5 cells treated with 100 μg/ml MEP extract for 6 h. The results showed that MEP increased the mRNA levels of metabolic enzymes CYP1A1 and CYP1B1, proinflammatory cytokines interleukin (IL)-1α, IL-6, and IL-11, fibroblast growth factor (FGF)-6 and FGF-9, vascular endothelial growth factor (VEGF)-D, oncogene fra-1, and tumor suppressor p21. In contrast, MEP decreased tumor suppressor Rb mRNA in CL5 lung epithelial cells. Treatment with 10 μM benzo(a)pyrene for 6 h altered gene expression profiles, in a manner similar to those by MEP. Induction of IL-1α, IL-6, IL-11, and FGF-9 mRNA by MEP and benzo(a)pyrene was concentration and time dependent. Cotreatment with 2 mM N-acetylcysteine blocked the MEP- and benzo(a)pyrene-mediated induction. Treatment with MEP or benzo(a)pyrene increased IL-6 and IL-11 releases to CL5 cell medium. Incubation of human lung fibroblast WI-38 with MEP- or benzo(a)pyrene-induced CL5 conditioned medium for 4 days stimulated cell growth of the fibroblasts. Inhalation exposure of rats to 1:10 diluted motorcycle exhaust 2 h daily for 4 weeks increased CYP1A1, FGF-9, and IL-1α mRNA in lung. This present study shows that MEP and benzo(a)pyrene can induce metabolic enzyme, inflammatory cytokine, and growth factor gene expression in CL5 cells and stimulate lung epithelium-fibroblast interaction.
ACCESSION #
20605987

 

Related Articles

  • Enhanced expression of fibroblast growth factor 2 in bone marrow cells and its potential role in the differentiation of hepatic epithelial stem-like cells into the hepatocyte lineage. Haga, Hiroaki; Saito, Takafumi; Okumoto, Kazuo; Ugajin, Satoshi; Sato, Chikako; Ishii, Rika; Nishise, Yuko; Ito, Junitsu; Watanabe, Hisayoshi; Saito, Koji; Togashi, Hitoshi; Kawata, Sumio // Cell & Tissue Research;Feb2011, Vol. 343 Issue 2, p371 

    The transplantation of bone marrow cells (BMCs) has been applied in liver regenerative cell therapy. However, details of the interaction between the transplanted BMCs and hepatic stem cells have not been elucidated. The aim of the present study was to investigate the interaction of BMCs with...

  • Nitric Oxide Mediates Interleukin-1-Induced Gene Expression of Matrix Metalloproteinases and Basic Fibroblast Growth Factor in Cultured Rabbit Articular Chondrocytes1. Sasaki, Kazuhiro; Hattori, Takako; Fujisawa, Takuo; Takahashi, Kojiro; Inoue, Hajime; Takigawa, Masaharu // Journal of Biochemistry;1998, Vol. 123 Issue 3, p431 

    We recently reported that nitric oxide (NO), which is produced by chondrocytes treated with interleukin-1β (IL-1), releases basic fibroblast growth factor (bFGF) stored in the matrix of articular chondrocytes. To clarify the mechanism of the IL-1-induced bFGF release, we investigated the...

  • FGF23 modulates the effects of erythropoietin on gene expression in renal epithelial cells. Yashiro, Mitsuru; Ohya, Masaki; Mima, Toru; Ueda, Yumi; Nakashima, Yuri; Kawakami, Kazuki; Ishizawa, Yohei; Yamamoto, Shuto; Kobayashi, Sou; Yano, Takurou; Tanaka, Yusuke; Okuda, Kouji; Sonou, Tomohiro; Shoshihara, Tomohiro; Iwashita, Yuko; Iwashita, Yu; Tatsuta, Kouichi; Matoba, Ryo; Negi, Shigeo; Shigematsu, Takashi // International Journal of Nephrology & Renovascular Disease;Apr2018, Vol. 11, p125 

    Background: FGF23 plays an important role in calcium-phosphorus metabolism. Other roles of FGF23 have recently been reported, such as commitment to myocardium enlargement and immunological roles in the spleen. In this study, we aimed to identify the roles of FGF23 in the kidneys other than...

  • Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury. Volckaert, Thomas; Dill, Erik; Campbell, Alice; Tiozzo, Caterina; Majka, Susan; Bellusci, Saverio; De Langhe, Stijn P. // Journal of Clinical Investigation;Nov2011, Vol. 121 Issue 11, p4409 

    During lung development, parabronchial SMC (PSMC) progenitors in the distal mesenchyme secrete fibroblast growth factor 10 (Fgf10), which acts on distal epithelial progenitors to promote their proliferation. β-catenin signaling within PSMC progenitors is essential for their maintenance,...

  • Analysis of resveratrol as a lung cancer chemopreventive agent in A/J mice exposed to benzo[a]pyrene. Berge, G.; Øvrebø, S.; Eilertsen, E.; Haugen, A.; Mollerup, S. // British Journal of Cancer;10/04/2004, Vol. 91 Issue 7, p1380 

    Resveratrol inhibits PAH bioactivation through reduced expression of the CYPIAI and CYPIBI genes in human bronchial epithelial cells. Ad libitum access to a diet containing resveratrol showed no effect on benzo[a]pyrene-induced lung tumorigenesis in A/J mice. Also, reweratrol did not change...

  • Quantification of Cytokeratin 5 mRNA Expression in the Circulation of Healthy Human Subjects and after Lung Transplantation. Nickerson, Derek W.; Presson, Angela P.; Weigt, Stephen S.; Gregson, Aric L.; Belperio, John A.; Gomperts, Brigitte N. // PLoS ONE;2009, Vol. 4 Issue 6, p1 

    Background: Circulating epithelial progenitor cells are important for repair of the airway epithelium in a mouse model of tracheal transplantation. We therefore hypothesized that circulating epithelial progenitor cells would also be present in normal human subjects and could be important for...

  • Localization of Basic Fibroblast Growth Factor mRNA in Melanocytic Lesions by In Situ Hybridization. Scott, Glynis; Stoler, Mark; Sarkar, Saumyendra; Halaban, Ruth // Journal of Investigative Dermatology;Mar1991, Vol. 96 Issue 3, p318 

    Basic fibroblast growth factor (bFGF) is a mitogen for normal human melanocytes and keratinocytes in culture. Experiments in vitro suggest that keratinocytes supply bFGF to melanocytes through a paracrine mechanism and that the aberrant expression of bFGF in melanomas confers growth independence...

  • Mice lacking CCAAT/enhancer-binding protein-α show hyperproliferation of alveolar type II cells and increased surfactant protein mRNAs. Sugahara, Kazuhiro; Iyama, Ken-Ichi; Kimura, Tatsuya; Sano, Kimihiko; Darlington, Gretchen J.; Akiba, Tsuguno; Takiguchi, Masaki // Cell & Tissue Research;Oct2001, Vol. 306 Issue 1, p57 

    The lung-specific surfactant proteins (SP) are essential for normal respiratory function. Transcription factors may play an important role in the regulation of surfactant proteins. The CCAAT/enhancer-binding protein (C/EBP) family consists of transcription factors that can stimulate expression...

  • Functional Expression of PEPT2 in the Human Distal Lung Epithelial Cell Line NCl-H441. Takano, Mikihisa; Sugimoto, Natsumi; Ehrhardt, Carsten; Yumoto, Ryoko // Pharmaceutical Research;Dec2015, Vol. 32 Issue 12, p3916 

    Purpose: The peptide transporter PEPT2 is expressed in alveolar type II epithelial cells. So far, however, no appropriate alveolar epithelial cell line for studying PEPT2 function has been known. In this study, we examined the functional expression of PEPT2 in the human distal lung epithelial...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics