TITLE

Quantitative plasma determination of a novel antiretroviral derivative of zidovudine by solid-phase extraction and high-performance liquid chromatography

AUTHOR(S)
Quevedo, Mario A.; Teijeiro, Silvina A.; Briñón, Margarita C.
PUB. DATE
June 2006
SOURCE
Analytical & Bioanalytical Chemistry;Jun2006, Vol. 385 Issue 2, p377
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A solid-phase extraction methodology, followed by high-performance liquid chromatography (HPLC) quantification with UV absorbance detection ( λ=267 nm), was developed in order to study the stability of 3′-azido-3′-deoxy-5′- O-isonicotinoylthymidine (AZT-Iso), a novel derivative of the antiretroviral AZT, in different matrixes. The half-lives ( t 1/2) for AZT-Iso were 1.19, 1.13 and 0.30 h for human, rat and rabbit plasma, respectively, and 14.91 and 25.49 h for potassium phosphate buffer (pH 7.4) and human serum albumin solution, respectively. The HPLC method proved to be selective, sensitive and accurate. Good recovery, linearity and precision were achieved using p-fluorophenol as an internal standard. The validity of this method was tested using synthetic mixtures of the intact drug with its decomposition products. In conclusion, the method presented is applicable to in vivo pharmacokinetics studies of AZT-Iso in rats.
ACCESSION #
20599041

 

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