TITLE

New insights from X-ray photoelectron spectroscopy into the chemistry of covalent enzyme immobilization, with glutamate dehydrogenase (GDH) on silicon dioxide as an example

AUTHOR(S)
Longo, Luigia; Vasapollo, Giuseppe; Guascito, Maria Rachele; Malitesta, Cosimino
PUB. DATE
May 2006
SOURCE
Analytical & Bioanalytical Chemistry;May2006, Vol. 385 Issue 1, p146
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A three-step process for immobilization of glutamate dehydrogenase (GDH) on the surface of silicon dioxide has been studied by X-ray photoelectron spectroscopy (XPS). The enzyme layer was deposited on the silicon dioxide surface after first exposing the surface to 3-aminopropyltriethoxysilane (3-APTS) and reacting the silylated surface with glutaraldehyde (GA). Fine XPS analysis, performed after each step of the chemical procedure, revealed unknown details of the step-by-step construction of the enzyme layer under different experimental conditions.
ACCESSION #
20535711

 

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