TITLE

Isocratic separation of ginsenosides by high-performance liquid chromatography on a diol column at subambient temperatures

AUTHOR(S)
Da-Wei Lou; Saito, Yoshihiro; Zarzycki, Pawe K.; Ogawa, Mitsuhiro; Jinno, Kiyokatsu
PUB. DATE
May 2006
SOURCE
Analytical & Bioanalytical Chemistry;May2006, Vol. 385 Issue 1, p96
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An improved high-performance liquid chromatographic method for separation of a number of ginsenosides has been developed. The influence of temperature (from 0 to 25�C) on the retention and separation of the ginsenosides was studied by applying a binary mobile phase (acetonitrile/water, 82:18 v/v) and a diol column (LiChrospher 100 Diol). The column temperature is one of the more important parameters for the retention and separation of the components investigated. Selected thermodynamic parameters, including changes of enthalpy (? H�) and entropy (? S�), were estimated from linear van�t Hoff plots, and possible retention mechanisms were discussed. Moreover, the best separation conditions were selected based on optimization criteria including maximum retention time ( t R max), minimum resolution ( R smin), and relative resolution product ( r). Temperature regions close to 14�C offered the highest selectivity and almost equal distribution of the ginsenosides peaks across the chromatogram. Under such isocratic conditions, excellent separation of chromatographic standards and selected ginseng samples was achieved in less than 16 min.
ACCESSION #
20535707

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics