TITLE

Eradication of Helicobacter pylori infection reverses E-cadherin promoter hypermehylation

AUTHOR(S)
Chan, A. O. O.; Peng, J. Z.; Lam, S. K.; Lai, K. C.; Yuen, M. F.; Cheung, H. K. L.; Kwong, Y. L.; Rashid, A.; Chan, C. K.; Wong, B. C.-Y.
PUB. DATE
April 2006
SOURCE
Gut;Apr2006, Vol. 55 Issue 4, p463
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: E-cadherin methylation is important in gastric carcinogenesis. Reversing hypermethylation may halt the carcinogenic process. We have previously reported that Helicobacter pylon infection is associated with E-cadherin methylation in chronic gastritis patients. Aim: To examine if eradication of H pylori could reverse E-cadherin methylation. Methods: Patients with dyspepsia and positive for H pylori infection, with a mucosal biopsy showing chronic active gastritis, were randomised to receive H pylori eradication therapy (group 1, n = 41) or no treatment (group 2, n = 40), and were followed up prospectively. Gastric mucosae were taken for methylation assay at week 0 (before treatment) and week 6 (after treatment). Archived specimens of intestinal metaplasia with H pylori infection (n = 22) and without (n = 19) were retrieved for methylation analysis. Methylation was assessed using methylation specific polymerase chain reaction and sequencing. Results: Methylation at E-cadherin was detected in 46% (19/41) and 17% (7/41) of patients at weeks 0 and 6, respectively, in group 1 (p=0.004); 78.9% (15/19) of specimens were unmethylated after eradication of H pylori. Mucosal biopsy showed chronic inactive gastritis in 35 patients, intestinal metaplasia in one, and normal mucosa in five at week 6. Methylation was detected in 47.5% (19/40) and 52.5% (21 /40( of patients at weeks 0 and 6, respectively, in group 2 (P = 0.5). Gastric mucosal biopsy showed persistent chronic active gastritis in all cases. Methylation frequency did not differ in H pylori positive or negative intestinal metaplastic specimens (72.7% v 63%; p = 0.5). Conclusion: H pylori eradication therapy could reverse methylation in patients with chronic gastritis. This demonstrates an environmental effect on methylation.
ACCESSION #
20509017

 

Related Articles

  • Nodular Gastritis: An Endoscopic Indicator of Helicobacter Pylori Infection. Ta-Chuan Liao // Digestive Diseases & Sciences;Oct2007, Vol. 52 Issue 10, p2662 

    Abstract  We prospectively assessed the relationship between nodular gastritis and Helicobacter pylori infection. Of 1409 adults who underwent endoscopy for persistent dyspepsia between June 2004 and August 2005, 41 (2.9%) patients were diagnosed with nodular gastritis (11 [27%]...

  • Kyoto global consensus report on Helicobacter pylori gastritis. Sugano, Kentaro; Tack, Jan; Kuipers, Ernst J.; Graham, David Y.; El-Omar, Emad M.; Soichiro Miura; Ken Haruma; Masahiro Asaka; Naomi Uemura; Malfertheiner, Peter // Gut;Sep2015, Vol. 64 Issue 9, p1353 

    Objective To present results of the Kyoto Global Consensus Meeting, which was convened to develop global consensus on (1) classification of chronic gastritis and duodenitis, (2) clinical distinction of dyspepsia caused by Helicobacter pylori from functional dyspepsia, (3) appropriate diagnostic...

  • Lack of Effect of Treating. Blum, André L.; Talley, Nicholas J.; O'Moráin, Colm; van Zanten, Sander Veldhuyzen; Labenz, Joachim; Stolte, Manfred; Louw, Japie A.; Stubberöd, Arild; Theodórs, Asgeir; Sundin, Marie; Bolling-Sternevald, Elisabeth; Junghard, Ola // New England Journal of Medicine;12/24/98, Vol. 339 Issue 26, p1875 

    Background: It is uncertain whether treatment of Helicobacter pylori infection relieves symptoms in patients with nonulcer, or functional, dyspepsia. Methods: We conducted a double-blind, multicenter trial of patients with H. pylori infection and dyspeptic symptoms (moderate-to-very-severe pain...

  • Campylobacter pylori and Non-ulcer Dyspepsia. Rokkas, T.; Pursey, C.; Uzoechina, E.; Dorrington, L.; Simmons, N. A.; Filipe, M. I.; Sladen, G. E. // American Journal of Gastroenterology;Nov1987, Vol. 82 Issue 11, p1149 

    Non-ulcer dyspepsia (NUD) is a poorly understood syndrome often present in association with gastritis. Among patients undergoing gastroscopy, some with NUD have a gastric mucosa colonized by the campylobacter-like organism, Campylobacter pylori. We therefore studied prospectively 55...

  • Effects of Sucralfate and Sulglycotide Treatment on Active Gastritis and Helicobacter pylori Colonization of the Gastric Mucosa in Non-Ulcer Dyspepsia Patients. Barbara, Luigi; Biasco, Guido; Capurso, Lucio; Dobrilla, Giorgio; Lalli, Alfredo; Paganelli, Gian Maria; Pallone, Francesco; Torsoli, Aldo // American Journal of Gastroenterology;Sep1990, Vol. 85 Issue 9, p1109 

    We conducted a double-blind randomized treatment study on patients affects by non-ulcer dyspepsia in whom multiple biopsy specimens showed active gastritis. Patients were given either 3 g/day of sucralfate (n = 39) or 600 mg/day of sulglycotide (n - 50) for 6 wk, a glycopeptide isolated from pig...

  • Helicobacter pylori infection and gastropathy: A comparison between Indonesian and Japanese patients. Abdullah, Murdani; Ohtsuka, Hiroyuki; Rani, Abdul Aziz; Sato, Tadashi; Syam, Ari F.; Fujino, Masayuki A.; Abraham, Philip // World Journal of Gastroenterology;10/21/2009, Vol. 15 Issue 39, p4928 

    AIM: To compare the effects of Helicobacter pylori (H pylori) infection on gastropathy between Indonesian and Japanese patients. METHODS: Biopsy specimens were obtained during upper gastrointestinal endoscopy from 167 subjects (125 Indonesians and 42 Japanese) with uninvestigated symptoms of...

  • Impact of Helicobacter pylori Infection and Microscopic Duodenal Histopathological Changes on Clinical Symptoms of Patients with Functional Dyspepsia. Mirbagheri, Seyed; Khajavirad, Nasim; Rakhshani, Nasser; Ostovaneh, Mohammad; Hoseini, Seyed; Hoseini, Vahid // Digestive Diseases & Sciences;Apr2012, Vol. 57 Issue 4, p967 

    Aim: To evaluate the microscopic histopathological changes in duodenal tissue and its relationship to the severity of symptoms in patients with functional dyspepsia while taking the effect of Helicobacter pylori ( H. pylori) infection into account. Methods: Several gastric and duodenal biopsy...

  • Does Helicobacter pylori-induced inflammation of gastric mucosa determine the severity of symptoms in functional dyspepsia? Turkkan, Ebru; Uslan, Ihsan; Acarturk, Gursel; Topak, Nevin; Kahraman, Ahmet; Dilek, Fatma Husniye; Akcan, Yusuf; Karaman, Ozcan; Colbay, Mehmet; Yuksel, Seref // Journal of Gastroenterology;2009, Vol. 44 Issue 1, p66 

    Inflammation induces some structural and biochemical alterations and oxidative damage in gastric tissue. In this study, we aimed to investigate the relationship among the severity of symptoms, oxidative stress, and grading scales of Helicobacter pylori-induced gastric inflammation in functional...

  • Additional corpus biopsy enhances the detection of Helicobacter pylori infection in a background of gastritis with atrophy. Lan, Hung-Chieh; Chen, Tseng-Shing; Li, Anna Fen-Yau; Chang, Full-Young; Lin, Han-Chieh // BMC Gastroenterology;2012, Vol. 12 Issue 1, p1 

    Background: The best sites for biopsy-based tests to evaluate H. pylori infection in gastritis with atrophy are not well known. This study aimed to evaluate the site and sensitivity of biopsy-based tests in terms of degree of gastritis with atrophy. Methods: One hundred and sixty-four (164)...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics