TITLE

Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light

AUTHOR(S)
Gurskaya, Nadya G.; Verkhusha, Vladislav V.; Shcheglov, Alexander S.; Staroverov, Dmitry B.; Chepurnykh, Tatyana V.; Fradkov, Arkady F.; Lukyanov, Sergey; Lukyanov, Konstantin A.
PUB. DATE
April 2006
SOURCE
Nature Biotechnology;Apr2006, Vol. 24 Issue 4, p461
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Green fluorescent protein (GFP) and GFP-like proteins represent invaluable genetically encoded fluorescent probes. In the last few years a new class of photoactivatable fluorescent proteins (PAFPs) capable of pronounced light-induced spectral changes have been developed. Except for tetrameric KFP1 (ref. 4), all known PAFPs, including PA-GFP, Kaede, EosFP, PS-CFP, Dronpa, PA-mRFP1 and KikGR require light in the UV-violet spectral region for activation through one-photon excitation—such light can be phototoxic to some biological systems. Here, we report a monomeric PAFP, Dendra, derived from octocoral Dendronephthya sp. and capable of 1,000- to 4,500-fold photoconversion from green to red fluorescent states in response to either visible blue or UV-violet light. Dendra represents the first PAFP, which is simultaneously monomeric, efficiently matures at 37 °C, demonstrates high photostability of the activated state, and can be photoactivated by a common, marginally phototoxic, 488-nm laser line. We demonstrate the suitability of Dendra for protein labeling and tracking to quantitatively study dynamics of fibrillarin and vimentin in mammalian cells.
ACCESSION #
20456296

 

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