An Improved Technique for Isolation of Environmental Vibrio cholerae with Epidemic Potential: Monitoring the Emergence of a Multiple- Antibiotic--Resistant Epidemic Strain in Bangladesh

Faruque, Shah M.; Islam, M. Johirul; Ahmad, Qazi Shafi; Biswas, Kuntal; Faruque, A. S. G.; Nair, G. Balakrish; Sack, R. Bradley; Sack, David A.; Mekalanos, John J.
April 2006
Journal of Infectious Diseases;4/1/2006, Vol. 193 Issue 7, p1029
Academic Journal
Predicting cholera epidemics through monitoring the environment for the presence of pathogenic Vibrio cholerae is complicated by the presence in water of a large number of mostly nonpathogenic V. cholerae strains. V. cholerae strains causing recent cholera epidemics in Bangladesh carry the sulfamethoxazole-trimethoprim (SXT) element, which encodes resistance to several antibiotics. Here, we show that the use of a culture medium containing streptomycin, sulfamethoxazole, and trimethoprim (the antibiotic selection technique [AST]) can significantly enhance the isolation of environmental V. cholerae O1 with epidemic potential (P < .001). The AST was also used to monitor the recent emergence and spread of a new multiple-antibiotic-resistant strain of V. cholerae in Bangladesh. The results of this study support the hypothesis that pre-epidemic amplification of pathogenic V. cholerae occurs in the human host and leads to the start of an epidemic cycle dominated by a single clone of V. cholerae that spreads rapidly through environmental waters.


Related Articles

  • Genetic Traits of Vibrio cholerae O1 Haitian Isolates That Are Absent in Contemporary Strains from Kolkata, India. Ghosh, Priyanka; Naha, Arindam; Pazhani, G. P.; Ramamurthy, T.; Mukhopadhyay, Asish K. // PLoS ONE;Nov2014, Vol. 9 Issue 11, p1 

    The world's worst cholera epidemic in Haiti (2010) coerced to trace the origin and dissemination of the causative agent Vibrio cholerae O1 for proper management of cholera. Sequence analysis of the Haitian strain showed several variations in the genes encoding cholera toxin B subunit (ctxB);...

  • Molecular epidemiology of Vibrio cholerae causing outbreaks & sporadic cholera in northern India. Taneja, Neelam; Sangar, Garima; Chowdhury, Goutam; Ramamurthy, T.; Mishra, Arti; Singh, Meenakshi; Sharma, Meera // Indian Journal of Medical Research;Oct2012, Vol. 136 Issue 4, p656 

    Background & objectives: Several outbreaks of cholera have been reported in Chandigarh region during a span of seven years from 2002-2008. The genetic characteristics of Vibrio cholerae isolates obtained during these outbreaks have not been adequately studied. The aim of this study was to do...

  • Vibrio cholerae O139 in Calcutta, 1992-1998: Incidence, Antibiograms, and Genotypes. Basu, Arnab; Garg, Pallavi; Datta, Simanti; Chakraborty, Soumen; Bhattacharya, Tanuja; Khan, Asis; Ramamurthy, T.; Bhattacharya, S.K.; Yamasaki, Shinji; Takeda, Yoshifumi; Nair, G. Balakrish // Emerging Infectious Diseases;Mar/Apr2000, Vol. 6 Issue 2, p139 

    We report results of surveillance for cholera caused by Vibrio cholerae O139 from September 1992, when it was first identified, to December 1998. V. cholerae O139 dominated as the causative agent of cholera in Calcutta during 1992-93 and 1996-97, while the O1 strains dominated during the rest of...

  • A 4-Year Study of the Epidemiology of Vibrio cholerae in Four Rural Areas of Bangladesh. Sack, R. Bradley; Siddique, A. Kasem; Longini Jr., Ira M.; Nizam, Azhar; Yunus, Md.; Islam, M. Sirajul; Morris Jr., J. Glenn; Ali, Afsar; Huq, Anwar; Nair, G. Balakrish; Qadri, Firdausi; Faruque, Shah M.; Sack, David A.; Colwell, Rita R. // Journal of Infectious Diseases;1/1/2003, Vol. 187 Issue 1, p96 

    How Vibrio cholerae spreads around the world and what determines its seasonal peaks in endemic areas are not known. These features of cholera have been hypothesized to be primarily the result of environmental factors associated with aquatic habitats that can now be identified. Since 1997,...

  • Red Cholera.  // Time;Sep1961, Vol. 78 Issue 9, p53 

    The article reports on the Cholera outbreak caused by the tiny microbe Vibrio comma in the Kwangtung province in China. It notes that Hong Kong health officials were furious at the Communist authorities who kept the epidemic secret for months which could be carried by refugees escaping to Hong...

  • Three Cases of Bacteremia Caused by Vibrio cholerae O1 in Blantyre, Malawi.  // Emerging Infectious Diseases;Nov/Dec2001, Vol. 7 Issue 6, p1059 

    Reports on a number of cases of bacteremia which occurred in the context of a community outbreak of cholera diarrhea in Blantyre, Malawi. Information on Vibrio cholerae; Discussion on the cholera outbreak; Details of the genomic analysis.

  • Cholera in United States Associated with Epidemic in Hispaniola. Newton, Anna E.; Heiman, Katherine E.; Schmitz, Ann; Török, Tom; Apostolou, Andria; Hanson, Heather; Gounder, Prabhu; Bohm, Susan; Kurkjian, Katie; Parsons, Michele; Talkington, Deborah; Stroika, Steven; Madoff, Lawrence C.; Elson, Franny; Sweat, David; Cantu, Venessa; Akwari, Okey; Mahon, Barbara E.; Mintz, Eric D. // Emerging Infectious Diseases;Nov2011, Vol. 17 Issue 11, p2166 

    Cholera is rare in the United States (annual average 6 cases). Since epidemic cholera began in Hispaniola in 2010, a total of 23 cholera cases caused by toxigenic Vibrio cholerae O1 have been confirmed in the United States. Twenty-two case-patients reported travel to Hispaniola and 1 reported...

  • SXT constin among Vibrio cholerae isolates from a tertiary care hospital. Pande, Kavita; Mendiratta, Deepak Kumar; Vijayashri, Deotale; Thamke, Dipak C.; Narang, Pratibha // Indian Journal of Medical Research;Mar2012, Vol. 135 Issue 3, p346 

    Background & objectives: The SXT element, also known as 'constin' (conjugable, self transmissible, integrating element) is an integrating conjugative element (ICE) in Vibrio cholerae discovered in the chromosome of epidemic V. cholerae O139 strain MO10 (SXTMO10) which arose in late 1992 in...

  • Identification of Vibrio cholerae Serogroup O1, Serotype Inaba, Biotype El Tor Strain -- Haiti, March 2012. Brunkard, Joan M.; Talkington, Deborah F. // MMWR: Morbidity & Mortality Weekly Report;5/4/2012, Vol. 61 Issue 17, p309 

    The article reports on two strains of Vibrio cholerae that were identified in Haiti in March 2012. The outbreak of cholera in Haiti was confirmed on October 20, 2010. But in March, two new strains were collected and were characterized as Inaba serotypes. The article discusses similarities and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics