Diurnal variation and age-related changes of bone turnover markers in female Göttingen minipigs

H. Tsutsumi; K. Katagiri; M. Morimoto; T. Nasu; M. Tanigawa; K. Mamba
October 2004
Laboratory Animals;Oct2004, Vol. 38 Issue 4, p439
Academic Journal
We investigated diurnal variation and age-related changes in bone turnover markers in female Göttingen minipigs. Ten females, 6–9 months of age, were used for confirmation of diurnal variation. Blood was collected at 3 h intervals for 24 h, and bone-specific alkaline phosphatase and intact osteocalcin (OC) levels were determined by enzyme immunoassay and radioimmunoassay, respectively. Urine was collected at 3 h intervals for 24 h using a tray attached to the bottom of the cage. The levels of N-terminal telopeptide of type I collagen (NTX) were determined by enzyme immunoassay. Pyridinoline and deoxypyridinoline were measured by high performance liquid chromatography. OC and NTX exhibited diurnal variation (Kruskal–Wallis test, P < 0.05), with the highest and lowest levels at 18:00 h (76.7 ± 26.2 ng/ml) and 06:00 h (44.3 ± 10.3 ng/ml), and at 03:00–05:59 h (550.4 ± 82.4 nmol/μmol Cr) and 12:00–14:59 h (297.8 ± 152.5 nmol/μmol Cr), respectively. In the study of age-related changes, blood and urine samples from 66 females (age range, 3–76 months) were examined to determine the bone turnover markers. All markers showed high correlations with age (0.569 < R2 < 0.818). High levels of bone turnover markers were observed in young animals, decreasing with age (Kruskal–Wallis test, P < 0.01). The diurnal variation and age-related changes revealed in the present study will be useful in studies of bone diseases using female Göttingen minipigs.


Related Articles

  • Effect of some physico-chemical conditions on an immunoassay for viable Escherichia coli. Jurkevica, Agnese; Halsall, H. Brian; Seliskar, Carl J.; Heineman, William R. // Analytical & Bioanalytical Chemistry;Aug2010, Vol. 397 Issue 7, p3133 

    Viable Escherichia coli can be detected by an immunoassay in which live bacteria captured on antibody-coated paramagnetic beads are induced to synthesize the enzyme β-galactosidase, which catalyzes the hydrolysis of the slightly fluorescent substrate 4-methyl umbelliferyl-β- d-galactoside...

  • Discrepancies between a new highly sensitive Toxoplasma gondii ELISA assay and other reagents: interest of Toxo IgG Western blot. Lesl�, F.; Touafek, F.; Fekkar, A.; Mazier, D.; Paris, L. // European Journal of Clinical Microbiology & Infectious Diseases;Oct2011, Vol. 30 Issue 10, p1207 

    Immunodiagnostic assays are commonly used to screen for maternal toxoplasmic seroconversion during pregnancy. The introduction to the market of a new highly sensitive IgG assay, the Elecsys Toxo IgG test, has resulted in discrepancy issues with other immunoassays because of a lack of...

  • Assessing the diagnostic accuracy of immunodiagnostic techniques in the diagnosis of schistosomiasis japonica: a meta-analysis. Zhu, HuiPing; Yu, ChuanHua; Xia, Xin; Dong, GuoYing; Tang, Jie; Fang, Long; Du, Yukai // Parasitology Research;Oct2010, Vol. 107 Issue 5, p1067 

    Immunodiagnostic tests are commonly used in the diagnosis for schistosomiasis japonica in field situation. Their diagnostic effect, however, has never been evaluated in a systematic way. We set out to review the value of tests including enzyme-linked immunosorbent assay (ELISA) and indirect...

  • Evaluation of Buprenorphine CEDIA Assay versus GC-MS and ELISA using Urine Samples from Patients in Substitution Treatment. Böttcher, Michael; Beck, Olof // Journal of Analytical Toxicology;Nov/Dec2005, Vol. 29 Issue 8, p769 

    The study evaluates an immunochemical test based on cloned enzyme-donor immunoassay (CEDIA) technology for the use in clinical urine drug testing. The method was compared with an existing enzyme-linked immunosorbent immunoassay method and a gas chromatography-mass spectrometry method on urine...

  • Comparison between different methods of estimation of vitamin D. Pal, Mrinal; Datta, Subinay; Pradhan, Amit Kumar; Biswas, Lekha; Ghosh, Joydeep; Mondal, Partha; Rahut, Rajarshi; Chaudhuri, Arghya Ray; Sau, Sourav; Das, Soumi // Advances in Biological Chemistry;Oct2013, Vol. 3 Issue 5, p501 

    Background: Vitamin D is not only important for bone health but can also affect the development of several non-bone diseases. These findings have increased the need for determining vitamin D status in a convenient and cost-effective way. In order to determine the better method for detecting...

  • Detection of Galactomannan Antigenemia by Enzyme Immunoassay for the Diagnosis of Invasive Aspergillosis: Variables That Affect Performance. Marr, Kieren A.; Balajee, S. Arunmozhi; McLaughlin, Lisa; Tabouret, Marc; Bentsen, Christopher; Walsh, Thomas J. // Journal of Infectious Diseases;8/1/2004, Vol. 190 Issue 3, p641 

    Invasive aspergillosis (IA) is a frequent complication of blood or marrow transplantation. Previous studies have reported that the Aspergillus galactomannan enzyme immunoassay (GM EIA) may be a useful diagnostic tool for IA, but its sensitivity is variable. We examined the performance of the GM...

  • Adventures in Immunoassay. Hurst, Jeff // Scientific Computing & Instrumentation;Feb2001, Vol. 18 Issue 3, p12 

    Discusses the replacement of radioimmunoassay-based techniques with enzyme linked immunosorbent assay (ELISA) methods in immunoassay technology in the clinical sector. Function of ELISA in medical applications; Benefits and disadvantages of ELISA methods; Developments in the technology.

  • Immunoassay to detect enhanced carbamothioate degradation in soil Shea, Patrick J.; Takacs, James M.; Staswick, Paul E.; Hutchinson, Pamela J. // Weed Technology;Apr/Jun1993, Vol. 7 Issue 2, p396 

    No abstract available.

  • Urine test for measuring bone loss.  // Modern Medicine;Jul95, Vol. 63 Issue 7, p39 

    Reports on the availability of an enzyme-linked immunosorbent assay (ELISA) for measuring bone resorption via urine specimen in the United States. How the test works; Presentation of results; Applications.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics