TITLE

Increased Expression of Surfactant Protein A and D in Rheumatoid Arthritic Synovial Fluid

AUTHOR(S)
Kankavi, Orhan
PUB. DATE
February 2006
SOURCE
Croatian Medical Journal;2006, Vol. 47 Issue 1, p155
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Aim To determine the concentrations of surfactant protein (SP)-A and SP-D in synovial fluid samples from patients with rheumatoid arthritis and healthy controls and correlate them with rheumatoid factor, C-reactive protein (CRP), immunoglobulin (Ig) A, IgM, and IgG, total protein, and total lipid content. Methods The concentrations of SP-A, SP-D, CRP, IgA, IgM, IgG, and rheumatoid factor were measured in the synovial fluid of 7 patients with rheumatoid arthritis and 3 samples of healthy synovial fluid obtained at autopsy. The bands obtained by specific antibodies in Western blotting to determine the surfactant protein concentration were analyzed densitometrically and synovial fluid total phospholipids and protein content were analyzed spectrophotometrically. Results Patients with rheumatoid arthritis had increased concentrations of proteins and lipids in the synovial fluid, which correlated with 3.5-fold increase in SP-A and 6.1-fold increase in SP-D concentrations. Total protein content in synovial fluid samples from patients with rheumatoid arthritis showed a 2.1-fold increase and phospholipid content showed 7.0-fold increase in comparison with samples of healthy synovial fluid. Rheumatoid factor, CRP, IgA, IgM, and IgG concentrations in synovial fluid samples from patients with rheumatoid arthritis were 40-2660 KIU/L, 4-35 mg/L, 0.10-2.70 g/L, 0.50-1.90 g/L, and 5.3-15.4 g/L respectively. Conclusion SP-A and SP-D were present and expressed in various degrees in the synovial fluid of patients with rheumatoid arthritis. SP-A and SP-D may play role in the initiation of immune system and joint inflammation, and may be an integral component of synovial fluid and provide insights for in innate and adaptive immunity within the joint.
ACCESSION #
20026143

 

Related Articles

  • Synovial fluid ferritin in rheumatoid arthritis: an index or cause of inflammation? Blake, D.R.; Bacon, P.A. // British Medical Journal (Clinical Research Edition);1/17/1981, Vol. 282 Issue 6259, p189 

    Examines the correlation of synovial fluid ferritin concentrations with other indices of inflammatory activity. Content of iron in patients with rheumatoid arthritis; Outcome resulting from the incorporation of iron as ferritin in synovial cell lysosomes; Derivation of ferritin from the...

  • Synovial fluid expression of autoantibodies specific for RAGE relates to less erosive course of rheumatoid arthritis. R. Pullerits; M. Bokarewa; L. Dahlberg; A. Tarkowski // Rheumatology;Jul2007, Vol. 46 Issue 8, p1367 

    Objectives. The receptor for advanced glycation end products (RAGE) is expressed by many cells in joints of rheumatoid arthritis (RA) patients and interacts with a variety of pro-inflammatory ligands that are enriched in inflamed joint. The RAGE-ligand interaction leads to a sustained...

  • SERUM LEVELS OF ADIPONECTIN BEFORE AND AFTER ADMINISTRATION OF ANTI-TUMOR NECROSIS FACTOR AGENTS IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS. ABDUL-WAHID, K. M.; AL-DERZI, A. R.; AL-OSAMI, M. H.; AL-ANI, M. M. // International Journal of Immunology Research;2013, Vol. 3 Issue 2, p50 

    Rheumatoid arthritis is a chronic systemic inflammatory disease that affects many organs and tissues and principally attacks synovial joints. It is an autoimmune disease that produces inflammatory response of synovium secondary to hyperplasia of the synovial cells. Recent findings suggest that...

  • CPPD crystal deposition disease in patients with rheumatoid arthritis. Gerster, J.; Varisco, P.; Kern, J.; Dudler, J.; So, A. // Clinical Rheumatology;Aug2006, Vol. 25 Issue 4, p468 

    The aim of this study was to assess the frequency and the outcome of patients suffering from rheumatoid arthritis in which calcium pyrophosphate dihydrate (CPPD) crystal deposits were found to coexist in synovial fluid analysis. Such association was more frequent than previously believed with...

  • Trisomy 7 in synovial fluid cells of patients with rheumatoid arthritis. Tascioglu, Funda; Durak, Beyhan; Oner, Cengiz; Artan, Sevilhan // Rheumatology International;Oct2005, Vol. 25 Issue 8, p571 

    Objective: Recent studies revealed trisomy 7 as a chromosomal abnormality in non-neoplastic disorders such as rheumatoid arthritis (RA). In the present study, we investigated the presence of trisomy 7 in the synovial fluid cells of patients with RA using fluorescence in situ hybridisation (FISH)...

  • Serum and synovial fluid leptin levels and markers of inflammation in rheumatoid arthritis. Seven, Arzu; Güzel, Savaş; Aslan, Mahmure; Hamuryudan, Vedat // Rheumatology International;May2009, Vol. 29 Issue 7, p743 

    This study was designed to investigate the serum and synovial fluid leptin levels, and inflammatory markers in rheumatoid arthritis (RA) patients. Serum and synovial fluid leptin levels were significantly higher ( P > 0.05) in RA patients than control group; RA patients with moderate disease...

  • From Synovial Tissue to Peripheral Blood: Myeloid Related Protein 8/14 Is a Sensitive Biomarker for Effective Treatment in Early Drug Development in Patients with Rheumatoid Arthritis. Choi, Ivy Y.; Gerlag, Danielle M.; Holzinger, Dirk; Roth, Johannes; Tak, Paul P. // PLoS ONE;Aug2014, Vol. 9 Issue 8, p1 

    Objective: The change in number of CD68-positive sublining macrophages in serial synovial biopsies has been successfully used to discriminate on the group level between effective and ineffective treatment during early drug development in rheumatoid arthritis (RA) patients. Measurement of a...

  • A Polymorphism of ORAI1 rs7135617, Is Associated with Susceptibility to Rheumatoid Arthritis. Jeng-Hsien Yen; Che-Mai Chang; Yu-Wen Hsu; Chih-Hung Lee; Mei-Shin Wu; Daw-Yang Hwang; Ben-Kuen Chen; Hsien-Tzung Liao; Man-TzuMarcie Wu; Wei-Chiao Chang // Mediators of Inflammation;2014, Vol. 2014, p1 

    Rheumatoid arthritis (RA), a chronic inflammatory disease usually occurring in synovial tissues and joints, is highly associated with genetic and environmental factors. ORAI1, a gene related to cellular immune system, has been shown to be involved in the pathogenesis of chronic inflammatory...

  • Synovial Fluid Complement: Usefulness in Diagnosis and Classification of Rheumatoid Arthritis. Bunch, Thomas W.; Hunder, Gene G.; Offord, Kenneth; McDuffie, Frederic C. // Annals of Internal Medicine;Jul74, Vol. 81 Issue 1, p32 

    Presents a study which examined the relation between synovial fluid complement concentration and severity or prognosis of disease in patients with rheumatoid arthritis. Correlation between synovial fluid and severe arthritis; Discussion on the complement levels of synovial fluid in rheumatoid...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics