TITLE

Biomolecular interaction analysis under electrophoretic flow conditions

AUTHOR(S)
Kumpf, Michael; Gauglitz, Günter
PUB. DATE
March 2006
SOURCE
Analytical & Bioanalytical Chemistry;Mar2006, Vol. 384 Issue 5, p1129
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Combining the advantages of electrophoresis with the advantages of biomolecular interaction analysis (BIA) enables the biospecific detection of separated molecules; for example it permits differentiation between a complementary single-stranded DNA and a single nucleotide polymorphism. In order to integrate these two techniques, it is necessary to investigate whether it is possible to detect a biomolecular interaction under electrophoretic flow conditions. To this end a novel detection system was developed for electrophoresis that utilizes a label-free and time-resolved detection technique: reflectometric interference spectroscopy (RIfS). The biological functions of important analytes were investigated using this system. Although RIfS can be used as a postcolumn detector, it is also possible to use it to detect relevant substances under electrophoretic flow conditions. DNA–LNA, biotin–streptavidin and protein–protein interactions were detected using this coupled electrophoresis–RIfS set-up.
ACCESSION #
19933204

 

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