TITLE

Determination of fumonisins B1 and B2 in Portuguese maize and maize-based samples by HPLC with fluorescence detection

AUTHOR(S)
Lino, C. M.; Silva, L. J. G.; Pena, A. L. S.; Silveira, M. I.
PUB. DATE
March 2006
SOURCE
Analytical & Bioanalytical Chemistry;Mar2006, Vol. 384 Issue 5, p1214
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Fumonisins B1 (FB1) and fumonisin B2 (FB2) are the main members of a family of mycotoxins produced by Fusarium verticillioides, Fusarium proliferatum, and other fungi species of the section Liseola. The present work shows the results of comparative studies using two different procedures for the analysis of fumonisins in maize and maize-based samples. The studied analytical methods involve extraction with methanol/water, dilution with PBS, and clean-up through immunoaffinity columns. Two reagents ( o-phthaldialdehyde and naphthalene-2,3-dicarboxaldehyde) were studied for formation of fluorescent derivatives. The separation and identification were carried out by high-performance liquid chromatography with fluorescence detection. The optimized method for analysis of fumonisins in maize involved extraction with methanol/water (80:20), clean-up with an immunoaffinity column, and derivatization with naphthalene-2,3-dicarboxaldehyde (NDA). The limit of detection was 20 μg kg−1 for FB1 and 15 μg kg−1 for FB2. Recoveries of FB1 and FB2 ranged from 79% to 99.6% for maize fortified at 150 μg kg−1 and 200 μg kg−1, respectively, with within-day RSDs of 3.0 and 2.7%. The proposed method was applied to 31 samples, and the presence of fumonisins was found in 14 samples at concentrations ranging from 113 to 2,026 μg kg−1. The estimated daily intake of fumonisins was 0.14 μg kg−1 body weight per day.
ACCESSION #
19933203

 

Related Articles

  • Fumonisin Measurement from Maize Samples by High-Performance Liquid Chromatography Coupled with Corona Charged Aerosol Detector. Szekeres, András; Budai, Andrea; Bencsik, Ottó; Németh, László; Bartók, Tibor; Szécsi, Árpád; Mesterházy, Ákos; Vágvölgyi, Csaba // Journal of Chromatographic Science;Nov2014, Vol. 52 Issue 10, p1181 

    Fumonisins are a class of mycotoxins produced mainly by Fusarium species, which is primary fungal contaminant of the maize and maize-derived products around the world. The B-series fumonisins (FB1, FB2 and FB3) are the most abundant and toxic constituent; thus, their levels are regulated...

  • Screening and Initial Binding Assessment of Fumonisin B1 Aptamers. McKeague, Maureen; Bradley, Charlotte R.; De Girolamo, Annalisa; Visconti, Angelo; Miller, J. David; DeRosa, Maria C. // International Journal of Molecular Sciences;Dec2010, Vol. 11 Issue 12, p4864 

    Fumonisins are mycotoxins produced by Fusarium verticillioides and F. proliferatum, fungi that are ubiquitous in corn (maize). Insect damage and some other environmental conditions result in the accumulation of fumonisins in corn-based products worldwide. Current methods of fumonisin detection...

  • Liquid Chromatographic Determination of Alternaria Toxins in Carrots. Solfrizzo, Michele; De Girolamo, Annalisa; Vitti, Carolina; Visconti, Angelo; van den Bulk, Ruud // Journal of AOAC International;Jan/Feb2004, Vol. 87 Issue 1, p101 

    Focuses on the liquid chromatographic determination of Alternaria toxins in carrots. Extraction of toxins from carrot with an acidified mixture of water-methanol-acetonitrile; Quantification of toxins by reversed-phase liquid chromatography with ultraviole4t diode array detection.

  • Molecular Characterization of Fusarium globosum Strains from South African Maize and Japanese Wheat. Moses, Lorraine M.; Marasas, Walter F. O.; Vismer, Hester F.; De Vos, Lieschen; Rheeder, John P.; Proctor, Robert H.; Wingfield, Brenda D. // Mycopathologia;Oct2010, Vol. 170 Issue 4, p237 

    The fungus Fusarium globosum was first isolated from maize in South Africa and subsequently from wheat in Japan. Here, multiple analyses revealed that, despite morphological similarities, South African maize and Japanese wheat isolates of the fungus exhibit multiple differences. An amplified...

  • Fumonisin-Producing Strains of Fusarium: A Review of Their Ecophysiology. Marin, Sonia; Magan, Naresh; Ramos, Antonio J.; Sanchis, Vicente // Journal of Food Protection;Aug2004, Vol. 67 Issue 8, p1792 

    This review focuses on the fumonisin-producing Fusarium species and the ecophysiology of these species. The effects of environmental biotic and abiotic factors on germination, growth, and fumonisin B1 production by Fusarium verticillioides and Fusarium proliferatum have been investigated under...

  • Fumonisin profiles of Fusarium thapsinum and F. andiyazi, two Fusarium species commonly recovered from sorghum, grown on maize and sorghum patties.  // South African Journal of Science;Jul/Aug2005, Vol. 101 Issue 7/8, preceding p314 

    The article presents an abstract related to a study on fumonisin profiles of Fusarium thapsinum and F. andiyazi, two Fusarium species commonly recovered from sorghum, presented by scientists D. Thomas, H.F Vismer, and J.P. Rheeder of South Africa. Both fungi produce fumonisins in culture...

  • Processing and purity assessment of standards for the analysis of type-B trichothecene mycotoxins. Krska, R.; Schothorst, R. C.; Van Egmond, H. P.; Josephs, R. D.; Lepschy, J.; Pettersson, H.; Chan, D.; Berthiller, F.; Schuhmacher, R.; Kandler, W.; Parich, A.; Welzig, E. // Analytical & Bioanalytical Chemistry;Aug2005, Vol. 382 Issue 8, p1848 

    The lack of reliable, certified calibrant solutions for the Fusarium mycotoxins deoxynivalenol (DON), 3-acetyl-DON (3-Ac-DON), 15-acetyl-DON (15-Ac-DON) and nivalenol (NIV) is a serious drawback in the already problematic area of trichothecene analysis. For this reason, purified DON, 3-Ac-DON,...

  • Liquid Chromatographic Analysis of Aflatoxin Using Post-Column Photochemical Derivatization: Collaborative Study. Waltking, Arthur E.; Wilson, David // Journal of AOAC International;May/Jun2006, Vol. 89 Issue 3, p678 

    The article describes the liquid chromatographic analysis of aflatoxin by post-column photochemical derivatization using a photochemical reactor for enhanced detection system for derivatization (PHRED). The PHRED is a compact unit placed between the liquid chromatographic column and the detector...

  • Synchronous Analysis Method for Detection of Citrinin and the Lactone and Acid Forms of Monacolin K in Red Mold Rice. Chun-Lin Lee; Jyh-Jye Wang; Tzu-Ming Pan // Journal of AOAC International;May/Jun2006, Vol. 89 Issue 3, p669 

    The article describe the detection of mycotoxin citrinin and the lactone and acid forms of monacolin K by a synchronous analysis method using high-performance liquid chromatography. Monacolin K is a cholesterol-lowering agent found in Monascus product known as red mold rice. The total length of...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics