Microemulsion electrokinetic chromatographic determination of bufadienolides in toad venom and in traditional Chinese medicine

Luo Xingping; Zhai Zongde; Zhao Yanfang; Chen Liren; Li Yongmin
March 2006
Analytical & Bioanalytical Chemistry;Mar2006, Vol. 384 Issue 5, p1254
Academic Journal
A microemulsion electrokinetic chromatographic (MEEKC) method has been developed and validated for determination of resibufogenin and cinobufagin in toad venom and in traditional Chinese medicine prepared from the venom. The MEEKC method involved use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent, and butan-1-ol as co-solvent. To improve the separation, the effect of temperature and running buffer pH were evaluated. The optimized conditions (heptane 0.81% ( w/ w), SDS 3.31% ( w/ w), butan-1-ol 6.61% ( w/ w), and 10 mmol L−1 sodium tetraborate buffer, pH 9.2, and 298 nm as the detection wavelength) enabled useful and repeatable separation of the analytes.


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