TITLE

Separation and determination of sugars by reversed-phase high-performance liquid chromatography after pre-column microwave-assisted derivatization

AUTHOR(S)
Momenbeik, Fariborz; Khorasani, Jafar H.
PUB. DATE
January 2006
SOURCE
Analytical & Bioanalytical Chemistry;Jan2006, Vol. 384 Issue 3, p844
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In this study the possibility of derivatizing sugars using microwave irradiation was investigated. The amount of reagent, irradiation intensity, and derivatization time were optimized. In the derivatization of sugars with p-nitroaniline the reaction is complete within 5 min at 600 W when the p-nitroaniline-to-sugar and NaBH3CN-to-sugar mole ratios were above 1.4 and 3.1, respectively. A Doehlert design was used to optimize the mobile phase for separation of p-nitroaniline-labeled sugars; and the best separation was obtained by use of 0.01 mol L−1 acetate buffer at pH 4.40 containing 11.0% acetonitrile. Analysis using this method was highly sensitive and analysis time was short. Finally, a food sample was analyzed using the proposed method.
ACCESSION #
19718173

 

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