The role of glial cells and apoptosis of enteric neurones in the neuropathology of intractable slow transit constipation

Bassotti, G.; Villanacci, V.; Maurer, C. A.; Fisogni, S.; Di Fabio, F.; Cadei, M.; Morelli, A.; Panagiotis, T.; Cathomas, G.; Salerni, B.
January 2006
Gut;Jan2006, Vol. 55 Issue 1, p41
Academic Journal
Background: Idiopathic slow transit constipation is one of the most severe and often intractable forms of constipation. As motor abnormalities are thought to play an important pathogenetic role, studies have been performed on the colonic neuroenteric system, which rules the motor aspects of the viscus. Aims: We hypothesised that important neuropathological abnormalities of the large bowel are present, that these are not confined to the interstitial cells of Cajal and ganglion cells, and that the previously described reduction of enteric neurones, if confirmed, might be related to an increase in programmed cell death (apoptosis). Patients and methods: Surgical specimens from 26 severely constipated patients were assessed by conventional and immunohistochemical methods. Specific staining for enteric neurones, glial cells, interstitial cells of Cajal, and fibroblast-like cells associated with the latter were used. In addition, gangliar cell apoptosis was evaluated by means of indirect and direct techniques. Data from patients were compared with those obtained in 10 controls. Results: Severely constipated patients displayed a significant decrease in enteric gangliar cells, glial cells, and interstitial cells of Cajal. Fibroblast-like cells associated with the latter did not differ significantly between patients and controls. Patients had significantly more apoptotic enteric neurones than controls. Conclusion: Severely constipated patients have important neuroenteric abnormalities, not confined to gongliar cells and interstitial cells of Cajal. The reduction of enteric neurones may in part be due to increased apoptotic phenomena.


Related Articles

  • Chronic constipation: no more idiopathic, but a true neuropathological entity. Bassotti, Gabrio; Villanacci, Vincenzo; Rostami Nejad, Mohammad // Gastroenterology & Hepatology from Bed to Bench;Jul2011, Vol. 4 Issue 3, p109 

    Chronic constipation is still considered a functional or idiopathic disorder. However, in recent years there is evidence that its pathopysiological grounds may be actually due to a complex system of abnormalities of the enteric nervous system of these patients. In particular, as reported in this...

  • Glia. Chouard, Tanguy; Gray, Noah // Nature;11/11/2010, Vol. 468 Issue 7321, p213 

    The article discusses various reports published within the issue related to glial cells including one on gliotransmission, sectors of glial research and the role of glia to many diseases of nervous system.

  • Neurovascular Disease.  // Current Medical Literature: Neurology;2008, Vol. 24 Issue 4, p107 

    The article presents medical studies on neurovascular diseases published in various medical journals in 2008. These include "Ischemic postconditioning inhibits apoptosis after focal cerebral ischemia/reperfusion injury in the rat," by B. Xing, H. Chen, M. Zhang et al., "Transient global amnesia:...

  • Advances in Adjuvant Therapy Against Acute Bacterial Meningitis. Pomar, Virginia; Martínez, Sergi; Paredes, Roger; Domingo, Pere // Current Drug Targets - Infectious Disorders;Dec2004, Vol. 4 Issue 4, p303 

    Death and co-morbidity derived from acute bacterial meningitis remain unacceptably high and are mainly related to immune-mediated cerebral dysfunction. Cerebral edema, hydrocephalus and ischaemic cerebrovascular events are severe complications that eventually occur following the activation of a...

  • An Antiapoptotic Neuroprotective Role for Neuroglobin. Brittain, Thomas; Skommer, Joanna; Raychaudhuri, Subadhip; Birch, Nigel // International Journal of Molecular Sciences;Jun2010, Vol. 11 Issue 6, p2306 

    Cell death associated with mitochondrial dysfunction is common in acute neurological disorders and in neurodegenerative diseases. Neuronal apoptosis is regulated by multiple proteins, including neuroglobin, a small heme protein of ancient origin. Neuroglobin is found in high concentration in...

  • Immunological Approaches to Prevent Neuronal Apoptosis During Neuroinflammation. Murphy, F. J.; Keating, K. E.; Sheehan, D.; Hayes, I. // Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy A;Aug2005, Vol. 4 Issue 4, p367 

    Neurodegeneration is set to increase in parallel with society's age demographics. With the increasing appreciation of the role played by apoptosis in neurodegeneration, much effort has gone into understanding key effectors in this process and to develop therapies that reduce neuronal apoptosis,...

  • Caspase-1 activation of caspase-6 in human apoptotic neurons. Guo, H.; Pétrin, D.; Zhang, Y.; Bergeron, C.; Goodyer, C. G.; LeBlanc, A. C. // Cell Death & Differentiation;Feb2006, Vol. 13 Issue 2, p285 

    Active caspase-6 (Csp-6) induces cell death in primary cultures of human neurons and is abundant in the neuropathological lesions of Alzheimer's disease. However, the mode of Csp-6 activation is not known. Here, we show that the Csp-1 inhibitor, Z-YVAD-fmk specifically prevents activation of...

  • Mitochondrial Function and Dysfunction in Diabetic Peripheral Neuropathy: An Updated Overview of Studies to Implicate Physiological Basis of Neuropathological Condition. Jamwal, Nisha Rani; P., Kumar Senthil; Anup, Kumar; Prabha, Adhikari; P. S., Jeganathan; Mariella, D'Souza // International Physiology;Jan-Jun2014, Vol. 2 Issue 1, p25 

    This short communication article was aimed to provide an evidence-informed overview and a scoping review of the role of mitochondrial function/dysfunction in diabetic peripheral neuropathy (DPN). From the reviewed studies, it is apparently evident that mitochondria played a comprehensive role...

  • Inhibition of apoptosis in neuronal cells infected with Chlamydophila (Chlamydia) pneumoniae. Appelt, Denah M.; Roupas, Maria R.; Way, Deana S.; Bell, Marcus G.; Albert, Elizabeth V.; Hammond, Christine J.; Balin, Brian J. // BMC Neuroscience;2008, Vol. 9, Special section p1 

    Background: Chlamydophila (Chlamydia) pneumoniae is an intracellular bacterium that has been identified within cells in areas of neuropathology found in Alzheimer disease (AD), including endothelia, glia, and neurons. Depending on the cell type of the host, infection by C. pneumoniae has been...


Read the Article


Sign out of this library

Other Topics