TITLE

Gastroprotective effects d oral nucleotide administration

AUTHOR(S)
Belo, A.; Marchbank, T.; Fitgerald, A.; Ghosh, S.; Playford, R. J.
PUB. DATE
February 2006
SOURCE
Gut;Feb2006, Vol. 55 Issue 2, p165
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background and aims: Nucleotides form the building blocks of DNA and are marketed as dietary supplements, alone or in combination with other ingredients, to promote general health. However, there has been only limited scientific study regarding the true biological activity of orally administered nucleotides. We therefore tested their efficacy in a variety of models of epithelial injury and repair. Methods: Effects on proliferation ([3H] thymicline incorporation) and restitution (cell migration of wounded monolayers) were analysed using HT29 and IEC6 cells. The ability of a nucleotide mixture to influence gastric injury when administered orally and subcutaneously was analysed using a rat indomethacin (20 mg/kg) restraint model. Results: In both cell lines, cell migration was increased by approximately twofold when added at 1 mg/ml (p<0.01); synergistic responses were seen when a mixture of nucleotides was used. Cell proliferation was stimulated by adenosine monophosphate (AMP) in HT29, but not in IEC6, cells. Gastric injury was reduced by approximately 60% when gavaged at 4–16 mg/ml (p<0.05), concentrations similar to those likely to be found in consumers taking nucleotide supplements. Systemic administration of nucleotides was unhelpful. Conclusions: Nucleotides possess biological activity when analysed in a variety of models of injury and repair and could provide a novel inexpensive approach for the prevention and treatment of the iniurious effects of non steroidal anti-inflammatory drugs and other ulcerative conditions of the bowel. Further studies on their potential benefits (and risks) appear justified.
ACCESSION #
19552425

 

Related Articles

  • Putting the lid on phosphodiesterase 4. Houslay, Miles D.; Adams, David R. // Nature Biotechnology;Jan2010, Vol. 28 Issue 1, p38 

    The author offers insights on a study on the regulation of phosphodiesterase 4 (PDE4), the major enzyme for degrading cyclic adenosine monophosphate (cAmp)in cell, by phosphorylation and protein-protein interactions that can lead to the discovery of allosteric modulators with reduced side...

  • THE LIBERATION OF ADENYL COMPOUNDS FROM PERFUSED ORGANS BY COBRA VENOM. Kellaway, C. H.; Trethewie, E. R. // Australian Journal of Experimental Biology & Medical Science;Mar1940, Vol. 18 Issue 1, p63 

    Focuses on a study on the liberation of adenyl compounds from perfused organs by cobra venom. Methods used in the study; Results of a study; Significance of using cobra venom.

  • Extracellular Nucleases of Rhizopus stolonifer. Rangarajan, E. S.; Deshpande, R. A.; Shankar, V. // Asian Journal of Biochemistry;2008, Vol. 3 Issue 2, p52 

    Rhizopus stolonifer produces two extracellular nucleases viz., nuclease Rsn and RNAse Rs in a ratio of approximately 1:60, when grown on YPG medium. The purified nuclease Rsn is a high Mr (67 kDa), metal requiring multifunctional endonuclease with a substrate specificity in the order of...

  • Promotion and inhibition of mutation in pathogens. Devaraj, Maurice Samuel // Russian Open Medical Journal;2014, Vol. 3 Issue 1, p1 

    This paper presents the research findings of a sequence of three experiments that were used to assess the impact of a cell's stage in its life cycle on mutatability and its possible mechanism. The first experiment consisted of a modified fluctuation test (Luria-Delbruck) to test for concurrency...

  • AMP-activated protein kinase: a physiological off switch for murine gastric acid secretion. Sidani, Shafik; Kopic, Sascha; Socrates, Thenral; Kirchhoff, Philipp; Föller, Michael; Murek, Michael; Capasso, Anna; Geibel, John P. // Pflugers Archiv European Journal of Physiology;Nov2009, Vol. 459 Issue 1, p39 

    Adenosine monophosphate (AMP)-activated protein kinase (AMPK) has been shown to be a metabolic energy regulator in various cells. Activation is a direct result of rising AMP concentration coupled with falling adenosine triphosphate (ATP). AMPK activation during metabolic stress consequently...

  • Rapid induction of cAMP/PKA pathway during retinoic acid-induced acute promyelocytic leukemia cell differentiation. Zhao, Q.; Tao, J.; Zhu, Q.; Jia, P.-M.; Dou, A.-X.; Li, X.; Cheng, F.; Waxman, S.; Chen, G.-Q.; Chen, S.-J.; Lanotte, M.; Chen, Z.; Tong, J.-H. // Leukemia (08876924);Feb2004, Vol. 18 Issue 2, p285 

    The second messenger cyclic adenosine monophosphate (cAMP) plays an important role in cell proliferation, differentiation and apoptosis. In the present work, we evaluated the cAMP signaling in acute promyelocytic leukemia (APL) cells in the context of differentiation induced by all-trans...

  • Increased Interleukin-4 Production by Atopic Mononuclear Leukocytes Correlates with Increased Cyclic Adenosine Monophosphate - Phosphodiesterase Activity and Is Reversible by Phosphodiesterase Inhibition. Chan, Sai C.; Shi-Hua Li; Hanifin, Jon M. // Journal of Investigative Dermatology;May93, Vol. 100 Issue 5, p681 

    Previous studies have shown that leukocytes from patients with atopic dermatitis have increased levels of cyclic adenosine monophosphate (cAMP)-phosphodiesterase activity. This increased activity accounts for subnormal cAMP responses and correlates with increased in vitro immunoglobulin...

  • Inhibition of the Phosphodiesterase 4 (PDE4) Enzyme Reverses Memory Deficits Produced by Infusion of the MEK Inhibitor U0126 into the CAl Subregion of the Rat Hippocampus. Han-Ting Zhang; Yu Zhao; Ying Huang; Dorairaj, Nandakumar R.; Chandler, L. Judson; O'Donnell, James M. // Neuropsychopharmacology;Aug2004, Vol. 29 Issue 8, p1432 

    Cyclic AMP-specific phosphodiesterase 4 (PDE4), which is an integral component of NMDA receptor-mediated cAMP signaling, is involved in the mediation of memory processes. Given that NMDA receptors also mediate MEK/mitogen-activated protein kinase (MAPK, ERK) signaling, which is involved in...

  • Identification of Novel cAMP Responsive Element Modulator (CREM) Isoforms Expressed by Osteoblasts. Liu, F.; Huang, Y.-F.; Kream, B. E. // Calcified Tissue International;Aug2005, Vol. 77 Issue 2, p91 

    CREM, the cyclic adenosine monophosphate (cAMP) responsive element modulator, belongs to a multigene family of cAMP-responsive transcription factors. CREM encodes a variety of different isoforms by utilizing four promoters and a complex pattern of alternative messenger ribonucleic acid (mRNA)...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics