A look back: message in a test tube

Eisenstein, Michael
February 2006
Nature Methods;Feb2006, Vol. 3 Issue 2, p148
Academic Journal
The article reports on the availability of single-solution systems for use in rapid RNA preparation. Phenol-chloroform extraction was first achieved by M.G. Sevag who observed that chloroform and protein enter into an interaction in solution which excludes nucleic acids. Sevag developed a method for phase-dependent separation of nucleic acids from cell extracts. Research groups later worked with guanidirrium hydrochloride which proved to be a powerful denaturant for proteins while leaving nucleic acids intact. A technique which produced a lysis and purification solution that incorporates phenol, chloroform and guanidinium hydrochloride, which enable the purification of non-degraded RNA from animal tissues has become a commercial success.


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